A5045563961- Estrogen and related hormone effects
- Nitric Oxide and Endothelin Effects
- MicroRNA in disease regulation
- Menopause: Health Impacts and Treatments
- Atherosclerosis and Cardiovascular Diseases
- Inflammatory mediators and NSAID effects
- Angiogenesis and VEGF in Cancer
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Listeria monocytogenes in Food Safety
- Microbial Inactivation Methods
- Hormonal Regulation and Hypertension
- Adipose Tissue and Metabolism
- Renin-Angiotensin System Studies
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Cardiac electrophysiology and arrhythmias
- Sex and Gender in Healthcare
- Genetics, Aging, and Longevity in Model Organisms
- Cerebrovascular and Carotid Artery Diseases
- Cytokine Signaling Pathways and Interactions
- Hedgehog Signaling Pathway Studies
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Adipokines, Inflammation, and Metabolic Diseases
- Inflammasome and immune disorders
Universitat de València
2015-2024
INCLIVA Health Research Institute
2015-2024
Menéndez Pelayo International University
2020
Biomedical Research Institute
2015-2018
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer
2012-2016
Instituto de Química y Fisicoquímica Biológicas
2016
Consejo Nacional de Investigaciones Científicas y Técnicas
2016
Instituto Nacional del Tórax
2016
Hospital Clínico Universitario de Valencia
2008-2015
Hospital Clínic de Barcelona
2015
Listeria monocytogenes is a food-borne pathogen with high mortality rate that has also emerged as paradigm for intracellular parasitism. We present and compare the genome sequences of L. (2,944,528 base pairs) nonpathogenic species, innocua (3,011,209 pairs). found large number predicted genes encoding surface secreted proteins, transporters, transcriptional regulators, consistent ability both species to adapt diverse environments. The presence 270 149 strain-specific (clustered in 100 63...
REVIEW article Front. Physiol., 06 June 2012Sec. Vascular Physiology Volume 3 - 2012 | https://doi.org/10.3389/fphys.2012.00165
Abstract Despite the promising value of miRNAs in diagnostic and prognostic cardiovascular disease (CVD), recent meta-analyses did not support their potential. Methodological variances studies may interfere with miRNA profile affect results. This study determines if blood starting material is a source variance by performing paired comparison plasma serum expression primary associated CVD. Circulating yield was similar both serum, although significant increase observed patients...
Estradiol (E 2 ) acts on the endothelium to promote vasodilatation through release of several compounds, including prostanoids, which are products arachidonic acid metabolism. Among these, prostacyclin (PGI2) and thromboxane A2 (TXA2) exert opposite effects vascular tone. The role different estrogen receptors (ERs) in PGI2/TXA2 balance, however, has not been fully elucidated. Our study sought uncover whether E enhances basal production PGI2 or TXA2 cultured human umbilical vein endothelial...
Objective— Our study aims to determine the role of time menopause on vascular inflammation biomarkers and how it affects their modulation by estrogen raloxifene in postmenopausal women. Methods Results— Uterine arteries from 68 women were divided into 3 segments cultured for 24 hours tissue culture media containing 17β-estradiol (100 nmol/L), or vehicle. Assessment arterial concentration 13 inflammatory was performed multiplex immunobead-based assay. Aging per se has a positive correlation...
Introduction: Circulating extracellular histones acquire relevance as cytotoxic mediators in sepsis. Extracellular act damage-associated molecular patterns (DAMPs), which induce oxidative stress and NLRP3 inflammasome activation. Inflammasome mediates pyroptosis, a programmed cell death mechanism that produces inflammation. Despite evidence for activation immune cells during sepsis, it was unknown whether can produce endothelial inflammasomes Methods: We used human umbilical vein (HUVEC) to...
Summary Virulence genes from the facultative intracellular pathogen Listeria mono cytogenes are controlled by transcriptional regulator PrfA. Although PrfA synthesis is activated at 37°C, PrfA‐dependent expression remains low in rich medium. However, a strong induction of regulon observed when L. monocytogenes cultured presence charcoal. Here, we show that ‘charcoal effect’ results adsorption diffusible autorepressor substance released during exponential growth. Analyses using an strain...
ABSTRACT Most Listeria monocytogenes virulence genes are positively regulated by the PrfA protein, a transcription factor sharing sequence similarities with cyclic AMP (cAMP) receptor protein (CRP). Its coding gene, prfA , is itself via an autoregulatory loop mediated upstream PrfA-dependent plcA promoter. We have recently characterized * mutants from L. which, as result of single amino acid substitution in PrfA, Gly145Ser, constitutively overexpress and regulon. Here, we show that about 10...
Vascular effects of estradiol are being investigated because there controversies among clinical and experimental studies. DNA microarrays were used to investigate global gene expression patterns in cultured human umbilical vein endothelial cells (HUVEC) exposed 1 nmol/L for 24 hours. When compared control, 187 genes identified as differentially expressed with 1.9-fold change threshold. Supervised principal component analysis hierarchical cluster revealed the differences between control...
Aims Aging is among the major causes for lack of cardiovascular protection by estrogen (E2) during postmenopause. Our study aims to determine mechanisms whereby aging changes E2 effects on nitric oxide (NO) production in a mouse model accelerated senescence (SAM). Methods and Results Although we found no differences NO females SAM prone (SAMP, aged) compared resistant (SAMR, young), either DAF-2 fluorescence or plasmatic nitrite/nitrate (NO2/NO3), both cases, treatment increased SAMR but had...
Estrogen signalling plays an important role in vascular biology as it modulates vasoactive and metabolic pathways endothelial cells. Growing evidence has also established microRNA (miRNA) key regulators of function. Nonetheless, the estrogen regulation on miRNA profile cells is poorly understood. In this study, we aimed to determine how human explore different receptors (ERα, ERβ GPER) expression by estrogen.We used microarrays global umbilical vein (HUVEC) exposed a physiological...
The ruminant pathogen Listeria ivanovii differs from monocytogenes in that it causes strong, bizonal haemolysis and a characteristic shovel-shaped co-operative haemolytic ('CAMP-like') reaction with Rhodococcus equi. We cloned the gene responsible for differential properties of L. ivanovii, smcL. It encodes sphingomyelinase C (SMase) highly similar (> 50% identity) to SMases Staphylococcus aureus (beta-toxin), Bacillus cereus Leptospira interrogans. smcL was transcribed monocistronically...
Introduction The aim of this study was to determine prognostic factors for the risk new vascular events during first 6 months after acute myocardial infarction (AMI) or atherothrombotic stroke (AS). We were interested in role endothelial progenitor cells (EPC) and circulating (CEC) Methods Between February 2009 July 2012, 100 AMI 50 AS patients consecutively studied three Spanish centres. Patients with previously documented coronary artery disease ischemic strokes excluded. Samples collected...