A5059301351- Listeria monocytogenes in Food Safety
- Microbial Inactivation Methods
- Essential Oils and Antimicrobial Activity
- Plasma Applications and Diagnostics
- Yersinia bacterium, plague, ectoparasites research
- Vibrio bacteria research studies
- Antimicrobial Peptides and Activities
- Bacteriophages and microbial interactions
- Probiotics and Fermented Foods
- Bacterial biofilms and quorum sensing
- Vector-borne infectious diseases
- Salmonella and Campylobacter epidemiology
- Identification and Quantification in Food
- Biosensors and Analytical Detection
- Microbial infections and disease research
- Aquaculture disease management and microbiota
- Biochemical and Structural Characterization
- Magnetic and Electromagnetic Effects
- Bacillus and Francisella bacterial research
- Escherichia coli research studies
- Medical and Biological Ozone Research
- Invertebrate Immune Response Mechanisms
- Spaceflight effects on biology
- Enzyme Production and Characterization
- Plant-based Medicinal Research
Gamalei Institute of Epidemiology and Microbiology
2016-2025
Research Centre for Medical Genetics
2015-2024
National Research Centre
2018-2023
Central Research Institute of Epidemiology
2023
Ministry of Health of the Russian Federation
2011-2022
National Research Institute for Veterinary Virology and Microbiology of Russia
2019-2022
Nizhniy Novgorod Research Institute of Epidemiology and Microbiology named after Academician I.N. Blokhina
2021
Russian Academy of Sciences
2001-2020
Moscow Institute of Physics and Technology
2019
All-Russian Scientific Research Institute of Medicinal and Aromatic Plants
2017
Non-thermal (low-temperature) physical plasma is under intensive study as an alternative approach to control superficial wound and skin infections when the effectiveness of chemical agents weak due natural pathogen or biofilm resistance. The purpose this was test individual susceptibility pathogenic bacteria non-thermal argon measure treatments against in biofilms on surfaces. Overall, Gram-negative were more susceptible treatment than Gram-positive bacteria. For Pseudomonas aeruginosa,...
Inactivation of microorganisms by plasma a positive (PC) and negative corona (NC) discharge in air at atmospheric pressure was investigated. Gram‐positive bacteria Staphylococcus aureus Gram‐negative Pseudomonas aeruginosa were chosen for the inactivation. PC NC produce three types bactericidal agents, which are ultraviolet radiation (UV), neutral reactive species (R), electric field charged particles (E), respectively. We elucidated contribution each bioactive agent to inactivation S. P. ....
The need to develop new antimicrobial peptides is due the high resistance of pathogenic bacteria traditional antibiotics now and in future. creation synthetic peptide constructs a common successful approach development peptides. In this work, we use simple, flexible, scalable technique create hybrid containing amyloidogenic regions ribosomal S1 protein from Staphylococcus aureus. While cell-penetrating allows enter bacterial cell, site provides an effect by coaggregating with functional...
ABSTRACT There is now an urgent need to develop reliable, rapid, and cost‐effective methods for bacterial detection, particularly point‐of‐care applications. This study explores the unique properties of silicon nanowire (SiNW) arrays as a resistive sensing platform detecting Listeria innocua . Vertically aligned SiNWs, fabricated via metal‐assisted chemical etching, exhibited high sensitivity adsorption. Conductance measurements revealed more than 10‐fold increase concentrations rose from 10...
Summary Virulence genes from the facultative intracellular pathogen Listeria mono cytogenes are controlled by transcriptional regulator PrfA. Although PrfA synthesis is activated at 37°C, PrfA‐dependent expression remains low in rich medium. However, a strong induction of regulon observed when L. monocytogenes cultured presence charcoal. Here, we show that ‘charcoal effect’ results adsorption diffusible autorepressor substance released during exponential growth. Analyses using an strain...
Summary PrfA, a transcription factor structurally related to Crp/Fnr, activates Listeria monocytogenes virulence genes during intracellular infection. We report two new PrfA* mutations causing the constitutive overexpression of PrfA regulon. Leu‐140Phe lies in αD adjacent DNA‐binding motif C‐terminal domain, like previously characterized mutation (Gly‐145Ser). Ile‐45Ser, contrast, maps N‐terminal β‐roll, structure similar that Crp cAMP binding site. The vitro transcriptional properties...
Combining antimicrobial peptides (AMPs) with cell-penetrating (CPPs) has shown promise in boosting potency, especially against Gram-negative bacteria. We examined the CPP-AMP interaction distinct bacterial types based on cell wall differences. Our investigation focused AMPs incorporating penetratin CPP and dihybrid containing both TAT protein fragments from human immunodeficiency virus Antennapedia peptide (Antp). Assessment of TAT-AMP, AMP-Antp, TAT-AMP-Antp revealed their potential...
The gram-positive pathogenic bacterium Listeria monocytogenes is widely spread in the nature. L. was reported to be isolated from soil, water, sewage and sludge. Listeriolysin O (LLO) a major virulence factor. In course of infection mammals, LLO required for intracellular survival apoptosis induction lymphocytes. this study, we explored potential promote interactions between ubiquitous inhabitant natural ecosystems bacteriovorous free-living ciliate Tetrahymena pyriformis. Wild type reduced...
Non-thermal plasma (NTP) consists of a huge amount biologically active particles, whereas its temperature is close to ambient. This combination allows one use NTP as perspective tool for solving different biomedical tasks, including antitumor therapy. The treatment tumor cells with caused dose-dependent effects, such growth arrest and apoptosis. However, while the outcome has been established, molecular mechanisms interaction between eukaryotic have not thoroughly studied thus far. In this...
Abstract Plasma‐activated water mist (PAWM) is obtained by the ignition of plasma within an air–vapor mixture. PAWM demonstrates significant antibacterial properties, decreasing loads foodborne pathogens a factor 35.5 for Listeria monocytogenes , 166 Salmonella Typhimurium, and 266 Escherichia coli O157:H7 15 s. Bacterial biofilms have similar species‐dependant susceptibility. Biofilms L. E. are destroyed 44%, 77%, 71%, respectively, after being treated 2 min. Obtained results suggest...
Non-thermal plasma (NTP) is a flow of partially ionized argon gas at an ambient macroscopic temperature and microbicidal for bacteria, viruses fungi. Viability the Gram-negative obligate intracellular bacterial parasite Chlamydia trachomatis its host cells was investigated after NTP treatment. treatment C. extracellular elementary bodies (EBs) diminished concentration infectious bacteria by factor 9×104, as established parallel infection murine fibroblast McCoy with treated control EBs....
We used multivirulence locus sequence typing to analyze 68 Yersinia pseudotuberculosis isolates from patients in Russia during 1973-2014, including 41 with Far East scarlet-like fever. Four genotypes were found responsible, 1 being especially prevalent. Evolutionary analysis suggests that epidemiologic advantages could cause this genotype's dominance.
Susceptibility of 117 L. monocytogenes strains isolated during three time periods (1950-1980; 2000-2005, and 2018-2021) to 23 antibiotics was tested by the disk diffusion method. All were sensitive aminoglycosides (gentamicin, kanamycin, neomycin, streptomycin), glycopeptides (vancomycin teicoplanin), clarithromycin, levofloxacin, amoxicillin/clavulanic acid, trimethoprim/sulfamethoxazole. Resistance clindamycin observed in 35.5% strains. carbapenems, imipenem meropenem found 4% 5% strains,...
Food products may be a source of Salmonella, one the main causal agents food poisoning, especially after emergence strains resistant to antimicrobial preparations. The present work dealt with investigation occurrence resistance preparations among S. enterica isolated from food. isolates belonged 11 serovars, which Infantis (28%), Enteritidis (19%), and Typhimurium (13.4%) predominated. were most commonly trimethoprim/sulfamethoxazole (n = 19, 59.38%), cefazolin 15, 46.86%), tetracycline 13,...
The escalating threat of multidrug-resistant pathogens necessitates innovative approaches to combat infectious diseases. In this study, we examined peptides R23FS*, V31KS*, and R44KS*, which were engineered include an amyloidogenic fragment sourced from the S1 protein S. aureus, along with one or two cell-penetrating peptide (CPP) components. We assessed antimicrobial efficacy these in a liquid medium against various strains both Gram-positive bacteria, including aureus (209P 129B strains),...
Listeriosis is one of the most significant humans and animals foodborne infectious diseases. Here, we characterized 48 Listeria monocytogenes strains isolated in territory inner Eurasia during second half 20th century. A total 23 (52.3%) were susceptible to nine antibiotics tested, 30.43%, 15.22%, 8.7% resistant penicillin G, ampicillin, enrofloxacin, respectively. We applied multilocus sequence typing (MLST) scheme determine phylogenetic positions strains. All but strain belonged II...
The development and testing of new antimicrobial peptides (AMPs) represent an important milestone toward the drugs that can inhibit growth pathogens multidrug-resistant microorganisms such as Pseudomonas aeruginosa, Gram-negative bacteria. Most AMPs achieve these goals through mechanisms disrupt normal permeability cell membrane, which ultimately leads to death pathogenic cell. Here, we developed a unique combination membrane penetrating peptide prone amyloidogenesis create hybrid peptide:...
The gram-positive bacterium Listeria monocytogenes is an important foodborne pathogen contaminating dairy products. Closely related to L. saprophytic spp. are also frequent contaminators of food and, particularly, To distinguish from nonpathogenic and other bacteria, a dot-immunoassay was developed. immunoassay based on the polyclonal antibody secreted form surface virulence-associated monocytogenes-specific InlB protein. increase production, bacteria were grown brain-heart infusion agar...
The pathogenic Gram-positive bacterium Listeria monocytogenes has been evolving into a few phylogenetic lineages. Phylogenetically defined substitutions were described in the L. virulence factor InlB, which mediates active invasion mammalian cells via interactions with surface receptors c-Met and gC1q-R. InlB internalin domain (idInlB) is central to c-Met. Here we compared activity of purified recombinant idInlB isoforms characteristic for lineage I II. Size exclusion chromatography...
The purpose of this work was to establish the influence a regime cold microwave argon plasma treatments on physiological characteristics human fibroblasts and keratinocytes. We used three regimes application: single treatment, double treatment with 48 h interval, daily for 3 d. Cell proliferation after application quantified in real time, immunohistochemistry viability cells determine changes their physiology. It established that frequency cell is important outcome. In samples treated...
Changes in bacterial physiology caused by the combined action of magnetic force and microgravity were studied Escherichia coli grown using a specially developed device aboard International Space Station. The morphology metabolism E. under spaceflight (SF) or (SF + MF) conditions compared with ground cultivated bacteria standard (control) (MF) conditions. SF, SF MF, MF provided up-regulation Ag43 auto-transporter cell auto-aggregation. visible clustering non-sedimenting that formed...