A5058020201- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Enzyme Production and Characterization
- Enzyme Structure and Function
- Studies on Chitinases and Chitosanases
- Antibiotic Resistance in Bacteria
- Peatlands and Wetlands Ecology
- Enzyme Catalysis and Immobilization
- Bacteriophages and microbial interactions
- Biofuel production and bioconversion
- Bacterial Genetics and Biotechnology
- Polar Research and Ecology
- Methane Hydrates and Related Phenomena
- Plant tissue culture and regeneration
- Plant Genetic and Mutation Studies
- Gut microbiota and health
- Fermentation and Sensory Analysis
- Coastal wetland ecosystem dynamics
- Amino Acid Enzymes and Metabolism
- Constructed Wetlands for Wastewater Treatment
- Fungal and yeast genetics research
- Legume Nitrogen Fixing Symbiosis
- Protist diversity and phylogeny
- Bacillus and Francisella bacterial research
- Transgenic Plants and Applications
Russian Academy of Sciences
2015-2025
Federal Center Research Fundamentals of Biotechnology
2017
Bioengineering Center
2008
Members of the Acidobacteria are among most efficient colonizers acidic terrestrial habitats but key traits underlying their environmental fitness remain to be understood. We analyzed indigenous assemblages in a lichen-covered (pH 4.1) soil forested tundra dominated by uncultivated members subdivision 1. An isolate these bacteria with cells occurring within saccular chambers, strain SBC82 T , was obtained. The genome consists 7.11-Mb chromosome and four megaplasmids, encodes wide repertoire...
Members of the bacterial order Planctomycetales have often been observed in associations with Crustacea. The ability to degrade chitin, however, has never reported for any cultured planctomycetes although utilization N-acetylglucosamine (GlcNAc) as a sole carbon and nitrogen source is well recognized these bacteria. Here, we demonstrate chitinolytic capability member family Gemmataceae, Fimbriiglobus ruber SP5T, which was isolated from peat bog. As revealed by metatranscriptomic analysis...
Anaerobic enrichments from hypersaline soda lakes with chitin as substrate yielded five closely related anaerobic haloalkaliphilic isolates growing on insoluble by fermentation at pH 10 and salinities up to 3.5 M. The chitinolytic activity was exclusively cell associated. To better understand the biology evolutionary history of this novel bacterial lineage, genome type strain ACht1 sequenced. Analysis 2.6 Mb draft revealed enzymes chitin-degradation pathways, including secreted cell-bound...
Summary Phycisphaera ‐like WD2101 ‘soil group’ is one of the as‐yet‐uncultivated phylogenetic clades within phylum Planctomycetes . Members this clade are commonly detected in various terrestrial habitats. This study shows that represented major planctomycete groups 10 boreal peatlands, comprising up to 76% and 36% all ‐affiliated 16S rRNA gene reads raised bogs eutrophic fens respectively. These types peatlands displayed clearly distinct intra‐group diversity WD2101‐affiliated...
The phylum
Resistance to antimicrobial drugs is an urgent problem not only in public health, but also animal husbandry. The widespread use of antimicrobials feed additives one the main reasons for rapid spread antibiotic resistance microbiota gastrointestinal tract farm animals. To characterize genes (resistome), we performed metagenomic analysis feces 24 cattle from different regions Russia, including cows breeds and yaks. Animals differed type housing: year-round on pastures or barns conventional...
Anaerobic enrichment from sediments of hypersaline alkaline lakes in Wadi el Natrun (Egypt) with chitin resulted the isolation a fermentative haloalkaliphilic bacterium, strain ACht6-1, growing exclusively insoluble as substrate sodium carbonate-based medium at pH 8.5-10.5 and total Na(+) concentrations 0.4 to 1.75 M. The isolate had Gram-negative cell wall formed lipid cysts old cultures. chitinolytic activity was associated cells. Analysis 4.4 Mb draft genome identified pathways for...
Two strains of filamentous, colorless sulfur bacteria were isolated from bacterial fouling in the outflow hydrogen sulfide-containing waters a coal mine (Thiothrix sp. Ku-5) and on seashore White Sea AS). Metagenome-assembled genome (MAG) A52 was obtained sulfidic spring Volgograd region, Russia. Phylogenetic analysis based 16S rRNA gene sequences showed that all genomes represented genus Thiothrix. Based their average nucleotide identity digital DNA-DNA hybridization data these new isolates...
Large areas in Northern Russia are covered by extensive mires, which represent a complex mosaic of ombrotrophic raised bogs, minerotrophic and eutrophic fens, all close proximity to each other. In this paper, we compared microbial diversity patterns the surface peat layers neighbouring bogs fens that located within two geographically remote mire sites Vologda region using 16S rRNA gene sequencing. Regardless location, communities were highly similar other but clearly distinct from those...
Food products may be a source of Salmonella, one the main causal agents food poisoning, especially after emergence strains resistant to antimicrobial preparations. The present work dealt with investigation occurrence resistance preparations among S. enterica isolated from food. isolates belonged 11 serovars, which Infantis (28%), Enteritidis (19%), and Typhimurium (13.4%) predominated. were most commonly trimethoprim/sulfamethoxazole (n = 19, 59.38%), cefazolin 15, 46.86%), tetracycline 13,...
Planctomycetes of the genus Singulisphaera are common inhabitants soils and peatlands. Although described members this characterized as possessing hydrolytic capabilities, ability to degrade chitin has not yet been reported for these bacteria. In study, a novel representative, strain Ch08, was isolated from chitinolytic enrichment culture obtained boreal fen in Northern European Russia. The 16S rRNA gene sequence isolate displayed 98.2% similarity that acidiphila MOB10T. Substrate...
Sulphate-reducing bacteria, primarily Desulfovibrio, are responsible for the active generation of H2S in swine production waste. The model species sulphate reduction studies, Desulfovibrio vulgaris strain L2, was previously isolated from manure characterized by high rates dissimilatory reduction. source electron acceptors low-sulphate waste rate formation remains uncertain. Here, we demonstrate ability L2 to use common animal farming supplements including L-lysine-sulphate, gypsum and...
Bacteria of the genus Acinetobacter, with their numerous species common in various habitats, play a significant role as pathogens. Their ability to adapt different living conditions is largely due presence plasmids containing necessary adaptive genes. At same time diversity Acinetobacter and evolutionary dynamics have not been sufficiently studied. Here, we characterized 44 isolated from 5 permafrost A. lwoffii strains, examined relationship modern strains identified groups related plasmids....
Microbial life can be supported at subzero temperatures in permafrost up to several million years old. Genome analysis of strains isolated from provides a unique opportunity study microorganisms that have not previously come into contact with the human population. Acinetobacter lwoffii is typical soil bacterium has been increasingly reported as hospital pathogens associated bacteremia. In order identify specific genetic characteristics ancient permafrost-conserved A. and their differences...
We expressed a putative β -galactosidase Asac_1390 from hyperthermophilic crenarchaeon Acidilobus saccharovorans in Escherichia coli and purified the recombinant enzyme. is composed of 490 amino acid residues showed high sequence similarity to family 1 glycoside hydrolases various thermophilic Crenarchaeota. The maximum activity was observed at pH 6.0 93°C. half-life enzyme 90°C about 7 hours. displayed tolerance glucose exhibits hydrolytic towards cellobiose aryl glucosides. with p...
The multigene family encoding the 5S rRNA, one of most important structurally-functional part large ribosomal subunit, is an obligate component all eukaryotic genomes. rDNA has long been a favored target for cytological and phylogenetic studies due to inherent peculiarities its structural organization, such as tandem arrays repetitive units their high interspecific divergence. complex polyploid nature genome bread wheat, Triticum aestivum, technically difficult task sequencing clusters...