A5064835504- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Metal Extraction and Bioleaching
- Protist diversity and phylogeny
- Lipid Membrane Structure and Behavior
- Wastewater Treatment and Nitrogen Removal
- Environmental DNA in Biodiversity Studies
- Microbial Fuel Cells and Bioremediation
- Bacteriophages and microbial interactions
- Aquatic Invertebrate Ecology and Behavior
- Methane Hydrates and Related Phenomena
- Microbial infections and disease research
- Protein Interaction Studies and Fluorescence Analysis
- Marine Biology and Ecology Research
- Pancreatic function and diabetes
- Enzyme Production and Characterization
- Bacterial biofilms and quorum sensing
- Surfactants and Colloidal Systems
- Nanopore and Nanochannel Transport Studies
- Erythrocyte Function and Pathophysiology
- Pancreatic and Hepatic Oncology Research
- Phosphorus and nutrient management
- Cardiac electrophysiology and arrhythmias
- ATP Synthase and ATPases Research
Voronezh State University
2020-2024
Two strains of filamentous, colorless sulfur bacteria were isolated from bacterial fouling in the outflow hydrogen sulfide-containing waters a coal mine (Thiothrix sp. Ku-5) and on seashore White Sea AS). Metagenome-assembled genome (MAG) A52 was obtained sulfidic spring Volgograd region, Russia. Phylogenetic analysis based 16S rRNA gene sequences showed that all genomes represented genus Thiothrix. Based their average nucleotide identity digital DNA-DNA hybridization data these new isolates...
Two metagenome-assembled genomes (MAGs), obtained from laboratory-scale enhanced biological phosphorus removal bioreactors, were analyzed. The values of 16S rRNA gene sequence identity, average nucleotide and amino acid identity indicated that these genomes, designated as RT SSD2, represented two novel species within the genus Thiothrix, ‘Candidatus Thiothrix moscowensis’ singaporensis’. A complete set genes for tricarboxylic cycle electron transport chain indicates a respiratory type...
The metagenome of foulings from sulfidic spring "Serovodorodny" (Tatarstan, Russia), where members the genus Thiothrix was observed, sequenced. Representatives phyla Gammaproteobacteria, Cyanobacteria and Campilobacteriota dominated in microbial community. complete genome sp. KT assembled metagenome. It displayed 93.93-99.72% 16S rRNA gene sequence identity to other species. average nucleotide (ANI) и digital DNA-DNA hybridization (dDDH) showed that designated represents a new species within...
Representatives of the colorless sulfur bacteria genus Beggiatoa use reduced compounds in processes lithotrophic growth, which is accompanied by storage intracellular sulfur. However, it still unknown how transformation occurs representatives. Annotation genome leptomitoformis D-402 did not identify any genes for oxidation or reduction elemental By searching BLASTP, two putative persulfide dioxygenase (PDO) homologs were found B. leptomitoformis. In some heterotrophic prokaryotes, PDO...
Two metagenome-assembled genomes (MAGs), GKL-01 and GKL-02, related to the family Thiotrichaceae have been assembled from metagenome of bacterial mat obtained a sulfide-rich thermal spring in North Caucasus. Based on average amino acid identity (AAI) values genome-based phylogeny, MAG represented new genus within family. The GC content DNA (44%) differed significantly that other known members Thiothrix (50.1–55.6%). We proposed assign species ‘Candidatus Thiocaldithrix dubininis’ gen. nov.,...
Currently, the phylogeny of genus Thiothrix is based on comparative whole genome analysis because high homology 16S ribosomal RNA gene sequences within genus. We analyzed possibility using various conservative genes as phylogenetic markers for Thiothrix. found that levels similarity nucleotide tRNA(Ile)-lysidine synthase (tilS) and β subunit polymerase (rpoB) are in good agreement with average identity (ANI) values between genomes representatives The strains MK1, WS, DNT52, DNT53, H33 were...
The family Beggiatoaceae is currently represented by 25 genera in the Genome Taxonomy Database, of which only 6 have a definite taxonomic status. Two metagenome-assembled genomes (MAGs), WS_Bin1 and WS_Bin3, were assembled from metagenomes sulfur mats coating laminaria remnants White Sea. Using obtained MAGs, we first applied phylogenetic analysis based on whole-genome sequences to address systematics Beggiatoaceae, clarify taxonomy this family. According average nucleotide identity (ANI)...
Representatives of filamentous colorless sulfur-oxidizing bacteria often dominate in sulfide biotopes, preventing the diffusion toxic into water column. One most intriguing groups is a recently described Beggiatoa leptomitoformis including strains D-401 and D-402T. Both have identical genes encoding enzymes which are involved oxidation hydrogen thiosulfate. Surprisingly, B. strain not capable to grow lithotrophically presence reduced sulfur compounds accumulate elemental inside cells,...
Целью данной работы было доказать способность Azospirillum thiophilum BV-ST к метилотрофному росту. Для этого были использованы следующие методы: культивирование бактерий в гетеротрофных и метилотрофных условиях, аннотирование генома с использованием автоматического аннотатора RAST, спектрофотометрическое измерение ферментативной активности, экстракция РНК сорбирующих микроколонок, обратная транскрипция, qPCR. В геноме выявлены гены, кодирующие ферменты окисления метанола,...