A5048260596- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Studies on Chitinases and Chitosanases
- Microbial Community Ecology and Physiology
- Microbial Metabolites in Food Biotechnology
- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Genomics and Phylogenetic Studies
- Microbial metabolism and enzyme function
- Plant Pathogens and Fungal Diseases
- Enzyme Catalysis and Immobilization
- Phytochemical compounds biological activities
- Enzyme Structure and Function
- Plant Pathogens and Resistance
- Digestive system and related health
- Fungal and yeast genetics research
- Microbial bioremediation and biosurfactants
- Legume Nitrogen Fixing Symbiosis
- Mycorrhizal Fungi and Plant Interactions
- Lichen and fungal ecology
- Phytase and its Applications
- Amino Acid Enzymes and Metabolism
- Bacillus and Francisella bacterial research
- Probiotics and Fermented Foods
- Genetically Modified Organisms Research
Russian Academy of Sciences
2016-2025
Czech Academy of Sciences, Institute of Microbiology
2023
Yahoo (United Kingdom)
2016
Genetika
1998-2010
Academician Pilyugin Center
2001-2009
Université Paris-Sud
2004
The family Isosphaeraceae accommodates stalk-free planctomycetes with spherical cells, which can be assembled in short chains, long filaments, or aggregates. These bacteria inhabit a wide variety of terrestrial environments, among those the recently described Paludisphaera borealis PX4T that was isolated from acidic boreal wetlands. Here, we analyzed its finished genome comparison to three other members Isosphaeraceae: Isosphaera pallida IS1BT, Singulisphaera acidiphila DSM 18658T, and...
Planctomycetes of the family Gemmataceae are characterized by large genome sizes and cosmopolitan distribution in freshwater terrestrial environments but their ecological functions remain poorly understood. In this study, we a novel representative family, strain PL17T, which was isolated from littoral tundra wetland capable growth on xylan cellulose. Cells isolate were represented pink-pigmented spheres that multiplied budding occurred singly or short chains aggregates. Strain PL17T...
Aerobic chemoorganotrophic planctomycetes of the genus Schlesneria colonize a wide spectrum freshwater ecosystems. The only described species this genus, S. paludicola, is represented by ellipsoid-shaped, moderately acidophilic bacteria isolated from acidic peat bogs. Here, we characterize novel neutrophilic member mountain wetland, strain T3-172T. This isolate possesses unpigmented, non-motile, spherical cells, which grow on media containing some mono- and polysaccharides in pH range...
Abstract Multiple‐sequence alignment of glycoside hydrolase (GH) families 32, 43, 62, and 68 revealed three conserved blocks, each containing an acidic residue at equivalent position in all the enzymes. A detailed analysis site‐directed mutations so far performed on invertases (GH32), arabinanases (GH43), bacterial fructosyltransferases (GH68) indicated a direct implication residues Asp/Glu (block I), Asp II), Glu III) substrate binding hydrolysis. These are close space 5‐bladed β‐propeller...
α‐ l ‐Rhamnosidases catalyze the hydrolysis of terminal ‐rhamnose residues in various carbohydrates. The catalytic domains most these enzymes belong to families GH78 and GH106 glycoside hydrolases. In this study, we show that almost all genes encoding GH78‐ GH106‐containing proteins from members poorly characterized bacterial phylum Acidobacteria originated precursors belonging Bacteroidetes . Members display similar functional capabilities specialize on degradation plant‐derived organic...
Methyloferula stellata AR4 is an aerobic acidophilic methanotroph, which, in contrast to most known methanotrophs but similar Methylocella spp., possesses only a soluble methane monooxygenase. However, it differs from spp. by its inability grow on multicarbon substrates. Here, we report the draft genome sequence of this bacterium.
The family Gemmataceae accommodates aerobic, chemoorganotrophic planctomycetes, which inhabit various freshwater ecosystems, wetlands and soils. Here, we describe a novel member of this family, strain PX52T, was isolated from boreal eutrophic lake in Northern Russia. This isolate formed pink-pigmented colonies represented by spherical cells that occurred singly, pairs or aggregates multiplied budding. Daughter were highly motile. PX52T an obligate aerobic chemoorganotroph, utilized sugars...
Comparison of the amino acid sequences four families glycosyl hydrolases reveals that they are homologous and have several common conserved regions. Two these contain β-fructosidases (glycosyl hydrolase GH32 GH68) other two include α-L-arabinases β-xylosidases (families GH43 GH62). The latter proposed to be grouped together with former into β-fructosidase (furanosidase) superfamily. Several ORFs can considered as a fifth family superfamily on basis sequence similarity. It is shown for first...
Annotating genomes remains an hazardous task. Mistakes or gaps in such a complex process may occur when relevant knowledge is ignored, whether lost, forgotten overlooked. This paper exemplifies approach which could help to resuscitate meaningful data.We show that set of closely related sequences have been annotated as ornithine carbamoyltransferases are actually putrescine carbamoyltransferases. demonstration based on the following points : (i) use enzymatic data had overlooked, (ii)...
Planctomycetes of the genus Singulisphaera are common inhabitants soils and peatlands. Although described members this characterized as possessing hydrolytic capabilities, ability to degrade chitin has not yet been reported for these bacteria. In study, a novel representative, strain Ch08, was isolated from chitinolytic enrichment culture obtained boreal fen in Northern European Russia. The 16S rRNA gene sequence isolate displayed 98.2% similarity that acidiphila MOB10T. Substrate...
Abstract Background As a rule, about 1% of genes in given genome encode glycoside hydrolases and their homologues. On the basis sequence similarity they have been grouped into more than ninety GH families during last 15 years. The GH97 family has established very recently initially included only 18 bacterial proteins. However, evolutionary relationship encoding proteins this remains unclear, as well distribution among main groups living organisms. Results extensive search current databases...
The GH101 family is composed of endo-alpha-N-acetylgalactosaminidases and their homologues. Pairwise sequence comparison phylogenetic analysis allowed us to distinguish five six subfamilies in this family. Diverse domain structures were found among the members. Usually they have irreplaceable some optional domains. Iterative screening protein database revealed an evolutionary relationship catalytic with glycoside hydrolase domains from GH13, GH31, GH70 families. Among other homologous...
ABSTRACT Methylocapsa palsarum NE2 T is an aerobic, mildly acidophilic, obligate methanotroph. Similar to other species, it possesses only a particulate methane monooxygenase and capable of atmospheric nitrogen fixation. The genome sequence this typical inhabitant subarctic wetlands soils also contains genes indicative aerobic anoxygenic photosynthesis.
Abstract — Planctomycetes of the class Phycisphaerae are aerobic and anaerobic heterotrophic bacteria that colonize a wide range marine terrestrial habitats. Their functional roles in environment, however, still poorly understood. Humisphaera borealis M1803 T is one very few characterized planctomycetes this class. It also first described representative previously uncultured group WD2101, which commonly detected soils peatlands. This work analyzed genetic determinants define ability to grow...
Evolutionary connections were analyzed for endo-β-xylanases, which possess the GH10 family catalytic domains. A homology search yielded thrice as many proteins are available from Carbohydrate-Active Enzymes (CAZy) database. Lateral gene transfer was shown to play an important role in evolution of bacterial family, especially phyla Acidobacteria, Cyanobacteria, Planctomycetes, Spirochaetes, and Verrucomicrobia. In case Verrucomicrobia, 23 lateral transfers organisms other detected....