A5017749913- Listeria monocytogenes in Food Safety
- Microbial Inactivation Methods
- Essential Oils and Antimicrobial Activity
- Liver physiology and pathology
- Biosensors and Analytical Detection
- Liver Disease Diagnosis and Treatment
- Organ Transplantation Techniques and Outcomes
- Identification and Quantification in Food
- Escherichia coli research studies
- Synthesis of Tetrazole Derivatives
- Salmonella and Campylobacter epidemiology
- Probiotics and Fermented Foods
- Viral Infectious Diseases and Gene Expression in Insects
- Protein Interaction Studies and Fluorescence Analysis
- Gold and Silver Nanoparticles Synthesis and Applications
- Nanotechnology research and applications
- Metal-Organic Frameworks: Synthesis and Applications
- Viral gastroenteritis research and epidemiology
- Nanoparticles: synthesis and applications
- Enzyme Production and Characterization
- Retinal Development and Disorders
- Neuroscience and Neuropharmacology Research
- Cholinesterase and Neurodegenerative Diseases
- Thyroid Disorders and Treatments
- Vibrio bacteria research studies
Gamalei Institute of Epidemiology and Microbiology
2017-2023
Peoples' Friendship University of Russia
2020-2023
Central Research Institute of Epidemiology
2023
Ministry of Health of the Russian Federation
2017-2022
National Research Centre
2018
The gram-positive bacterium Listeria monocytogenes is an important foodborne pathogen contaminating dairy products. Closely related to L. saprophytic spp. are also frequent contaminators of food and, particularly, To distinguish from nonpathogenic and other bacteria, a dot-immunoassay was developed. immunoassay based on the polyclonal antibody secreted form surface virulence-associated monocytogenes-specific InlB protein. increase production, bacteria were grown brain-heart infusion agar...
The pathogenic Gram-positive bacterium Listeria monocytogenes has been evolving into a few phylogenetic lineages. Phylogenetically defined substitutions were described in the L. virulence factor InlB, which mediates active invasion mammalian cells via interactions with surface receptors c-Met and gC1q-R. InlB internalin domain (idInlB) is central to c-Met. Here we compared activity of purified recombinant idInlB isoforms characteristic for lineage I II. Size exclusion chromatography...
Listeria monocytogenes is motile at 22°C and non-motile 37°C. In contrast, expression of L. virulence factors low up-regulated Here, we studied a character near surface swimming (NSS) motility its effects on adhesion patterns invasion into epithelial cells. saprophytic counterpart innocua both grown showed similar NSS characteristics including individual velocities, trajectory lengths, residence times, an asymmetric distribution velocity directions. Similar correlated with patterns. Motile...
L. monocytogenes is a widespread facultative intracellular pathogen. The range of natural hosts that supporting persistence in the environment has not been fully established yet. In this study, we were interested potential to infect cells bats, which are being increasingly recognized as reservoir for microorganisms pathogenic humans and domestic animals. A stable epithelial cell line was developed from kidneys Pipistrellus nathusii, small bat widely distributed across Europe. wild-type...
The facultative intracellular pathogen Listeria monocytogenes is of major veterinary importance in small ruminants. Nevertheless, details L. interactions with cells ruminants are not fully established. To study the potential to infect sheep cells, we used finite kidney cell line (shKEC), which was infected wild-type strain EGDe. invasion efficiency 0.015 ± 0.004%. factor InlB critically important for invasion, and inlB gene deletion almost prevented into shKEC cells. Comparison...
Abstract Hepatocyte growth factor (HGF) is central to liver regeneration. The Internalin B (InlB) protein a virulence produced by the pathogenic bacterium Listeria monocytogenes . InlB known mimic HGF activity interacting with receptor (HGFR) and activating HGFR‐controlled signaling pathways. We expressed purified HGFR‐binding domain, InlB321/15, cloned from fully virulent clinical L strain. HGFR Erk1/2 phosphorylation was determined using Western blotting. capacity of InlB321/15 bind...
Listeria monocytogenes virulence factor InlB specifically interacts with the receptors c-Met and gC1q-R. Both are present in non-professional professional phagocytes, including macrophages. Phylogenetically defined isoforms differently support invasion into phagocytes. This work deals effects of on L. uptake intracellular proliferation human Three receptor binding domain (idInlB) were derived from phylogenetically distinct strains belonging to highly virulent CC1 (idInlBCC1),...
The facultative intracellular pathogen Listeria monocytogenes causes a severe food-borne infection in humans and animals. L. invasion factor InlB interacts with the tyrosine kinase c-Met via N-terminal internalin domain. Previously, distinct variants of domain (idInlB) have been described field isolates. Three were used to restore full-length expression strain EGDeΔinlB. Obtained isogenic strains tested assay intravenous, intraperitoneal, intragastric models mice. All idInlBs functional,...
HGF (hepatocyte growth factor)/HGFR (HGF receptor) signaling pathway is a key in liver protection and regeneration after acute toxic damage. Listeria monocytogenes toxin InlB contains HGFR-interacting domain functional analog of HGF. The aim this work was to evaluate the hepatoprotective activity domain. recombinant InlB321/15 purified from E. coli. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) test used measure mitogenic HepG2 cells. Activation MAPK- PI3K/Akt-pathways...
Background. Listeria monocytogenes is characterized by the presence of epidemic hypervirulent clones. A key feature L. its capacity to invade non-professional phagocytic cells. Hypervirulent clones are strongly associated with increased production and/or certain isoforms invasion factors InlA and InlB.
 The purpose study create a test system for InlB detection measure levels in isolates belonging clonal groups different virulence potential.
 Materials methods. was performed using...
Aim. Using the model of intragastric Listeria monocytogenes infection in pregnant mice to compare an input found nature variants invasion factor InlB perinatal listeriosis. Materials and methods. Mice on 12-16 days pregnancy were injected intragastrically with 108 CFU isogenic recombinant L. strains EGDeAinlB::InlB9 EGDeAinlB:: InlB14. The expressed naturally occurring variants, InlB9 In 72 h, subjected euthanasia evaluate bacterial loads internal organs. Results. Only strain, which InlB14,...
Surface-enhanced Raman Scattering (SERS) is a powerful vibrational optical spectroscopy method that allows highly sensitive detection of molecules at very low concentrations matter by amplifying the electromagnetic fields created excitation localized surface plasmons on noble metal nanostructures. In presented work manufacturing composite nanostructures silicon nanowires decorated with silver (AgSiNWs) has been developed. The SERS activity AgSiNWs for protein was investigated using human...
Цель обзора – систематизация литературных данных о структуре, функции тиреоглобулина (ТГ) и экспрессии гена ТГ. Общая молекулярная структура ТГ консервативна у всех позвоночных, а продуция гормонов щитовидной железы трийодтиронина (Т3) тироксина (Т4) инициируется в процессе протеолиза При доставке из тиреоцитов просвет фолликуловщитовидной множественные остатки тирозина могут йодироваться с образованием монойодтирозина и/или дийодтирозина. Под действием тиреотропного гормона активируются его...
The development of an environmentally friendly process for the synthesis metal nanoparticles is important step in field nanotechnology. One way to achieve this goal use biological systems. In study, silver were obtained using extracellular enzymes organisms Saccharomyces boulardi strain. effect pH medium on was studied. antimicrobial activity investigated.
The work proposes a solvothermal method for obtaining metal-organic framework structures (MOF) based on strontium formate, which have high sorption capacity and an actively developed porous surface. effect of the ratio zinc nitrate to formic acid precursors structure, shape size MOF has been studied — these properties make it possible use biomedical purposes. In addition, studies were carried out determine antibacterial obtained MOF.