A5086118642- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
- Pharmaceutical and Antibiotic Environmental Impacts
- Escherichia coli research studies
- Enterobacteriaceae and Cronobacter Research
- Antimicrobial Resistance in Staphylococcus
- Antibiotics Pharmacokinetics and Efficacy
- Antibiotic Use and Resistance
- Urinary Tract Infections Management
- Streptococcal Infections and Treatments
- Genomics and Phylogenetic Studies
- Bacterial biofilms and quorum sensing
- Salmonella and Campylobacter epidemiology
- Mycobacterium research and diagnosis
- Infections and bacterial resistance
- Pneumonia and Respiratory Infections
- Bacterial Genetics and Biotechnology
- Vibrio bacteria research studies
- Plant Pathogenic Bacteria Studies
- Clostridium difficile and Clostridium perfringens research
- Nosocomial Infections in ICU
- Gut microbiota and health
- Anorectal Disease Treatments and Outcomes
- Infective Endocarditis Diagnosis and Management
- Bacteriophages and microbial interactions
Justus-Liebig-Universität Gießen
2016-2025
German Center for Infection Research
2016-2025
Instituto de Salud Carlos III
2024
Centro de Investigación Biomédica en Red
2024
Instituto Ramón y Cajal de Investigación Sanitaria
2024
Hospital Universitario Ramón y Cajal
2024
Institute of Medical Microbiology and Hygiene
2011-2022
Centro de Investigação em Biodiversidade e Recursos Genéticos
2022
Universidade do Porto
2022
Universitätsklinikum Gießen und Marburg
2006-2022
Abstract Background Surgical site infection (SSI) continues to be a major source of morbidity and mortality in developing countries despite recent advances aseptic techniques. There is no baseline information regarding SSI our setting therefore it was necessary conduct this study establish the prevalence, pattern predictors surgical at Bugando Medical Centre Mwanza (BMC), Tanzania. Methods This cross-sectional prospective involving all patients who underwent surgery wards between July 2009...
Abstract Background Black elderberries ( Sambucus nigra L.) are well known as supportive agents against common cold and influenza. It is further that bacterial super-infection during an influenza virus (IV) infection can lead to severe pneumonia. We have analyzed a standardized elderberry extract (Rubini, BerryPharma AG) for its antimicrobial antiviral activity using the microtitre broth micro-dilution assay three Gram-positive bacteria one Gram-negative responsible infections of upper...
Abstract Background Surgical site infection (SSI) is the second most common infectious complication after urinary tract following a delivery by caesarean section (CS). At Bugando Medical Centre there has no study documenting epidemiology of SSI CS despite large number CSs performed and relatively occurrence SSIs. Methods This was prospective cohort involving pregnant women who underwent between October 2011 February 2012 at Centre. A total 345 were enrolled. Preoperative, intraoperative...
The burden of antimicrobial resistance (AMR) is rapidly growing across antibiotic classes, with increased detection isolates resistant to carbapenems. Data on the prevalence carbapenem in developing countries limited; therefore, this study, we determined carbapenemase genes among multidrug gram negative bacteria (MDR-GNB) isolated from clinical specimens a tertiary hospital Mwanza, Tanzania. A total 227 MDR-GNB were analyzed for genes. For each isolate, five different PCR assays performed,...
Introduction: The increasing incidence of infections caused by extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in sub-Saharan Africa is serious concern. Studies from countries with a highly industrialized poultry industry suggest the production-food-consumer chain as potential transmission route. In Africa, integrated studies at this human-animal interface are still missing. Aim: To determine molecular epidemiology ESBL-producing E. intestinal tract humans and rural Ghana....
Multiresistant Gram-negative bacteria producing extended-spectrum β-lactamases (ESBLs) are an emerging problem in human and veterinary medicine. This study focused on comparative molecular characterization of β-lactamase ESBL-producing Enterobacteriaceae isolates from central Hesse Germany. Isolates originated humans, companion animals (dogs cats) horses.In this 153 (83.6%) the (n = 183) 163 (91.6%) animal 178) were confirmed as ESBL producers by PCR subsequent sequencing amplicons....
Escherichia (E.) coli producing extended-spectrum beta-lactamases (ESBLs) are an increasing problem for public health. The success of ESBLs may be due to spread ESBL-producing bacterial clones, transfer ESBL gene-carrying plasmids or exchange encoding genes on mobile elements. This makes it difficult identify transmission routes and sources bacteria. objectives this study were compare the distribution genotypic phenotypic properties E. isolates from different animal human collected in...
Klebsiella pneumoniae strains expressing ESBLs are a predominant cause of hospital acquired infections. Here we describe the molecular epidemiology these isolates in tertiary Tanzania, as potential pathogens for neonatal infections.Between April 2009 and March 2010 all with phenotypic expression Extended Spectrum Beta Lactamase (ESBL) were collected characterized. Identification was done using house biochemical tests case ambiguous results confirmation API 20E. Susceptibility testing...
Species within the Enterobacter cloacae complex (ECC) include globally important nosocomial pathogens. A three-year study of ECC in Germany identified xiangfangensis as most common species (65.5%) detected, a result replicated by examining global pool 3246 isolates. Antibiotic resistance profiling revealed widespread and heteroresistance to antibiotic colistin detected mobile (mcr)-9 gene 19.2% all We show that properties depend on chromosomal arnBCADTEF cassette whose products catalyze...
ABSTRACT Carbapenem-resistant Pseudomonas aeruginosa and Acinetobacter spp. represent major threats have few approved therapeutic options. Non-fermenting Gram-negative isolates were collected from hospitalized inpatients 49 sites in 6 European countries between 01 January 2020 31 December underwent susceptibility testing against cefiderocol β-lactam/β-lactamase inhibitor combinations. Meropenem-resistant (MIC >8 mg/L), cefiderocol-susceptible analyzed by PCR, cefiderocol-resistant...
ABSTRACT Renal transplant recipients are predisposed to urinary tract infections caused by both common uropathogens and opportunistic bacteria resulting frequently in significant polymicrobial infections. In this study, a culture-independent 16S rRNA-based approach was established identify unusual, fastidious, or anaerobic investigate bacterial diversity specimens. Similarly sized amplicons encompassing the V6 V8 region of rRNA were analyzed with denaturing high-performance liquid...
Pigs have been the focus of worldwide spread colistin resistance. However, there is little information on transmission mcr-1 -containing bacteria into environment pig farms. We therefore rescreened environmental Escherichia coli isolates from surrounding farm areas three previously -positive swine herds in Germany.Thirty-five mixed bacterial cultures obtained boot swabs, flies, dog faeces and manure farms Germany 2011-12 were non-selectively recultivated presence gene was checked by...
The increased presence of extended-spectrum beta-lactamase (ESBL)-producing bacteria in humans, animals, and their surrounding environments is global concern. Currently there limited information on ESBL rural farming communities worldwide. We performed a cross-sectional study Mwanza, Tanzania, involving 600 companion domestic farm animals between August/September 2014. Rectal swab/cloaca specimens were processed to identify ESBL-producing Enterobacteriaceae. detected 130 (21.7%) carrying...
The study was conducted to establish predictors of extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-PE) neonatal sepsis and mortality in a tertiary hospital, Tanzania. Between July December 2016, blood culture performed neonates with clinical features neonates/mothers/guardians were screened for ESBL colonization. Selected isolates underwent whole genome sequencing investigate relatedness. Logistic regression analysis determine ESBL-PE associated mortality. Neonatal...
Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae commonly cause infections worldwide. BlaCTX-M-15 has been detected in hospital isolates Mwanza, Tanzania. Little is known regarding the faecal carriage of ESBL and blaCTX-M-15 allele among humans community developing countries. A cross-sectional study involving 334 from settings Mwanza City was conducted between June September 2014. Stool specimens were collected processed to detect producing enterobacteriaceae. confirmed...
Extended-Spectrum Beta-Lactamase (ESBL)-producing bacteria are a common cause of healthcare and community-associated infections worldwide. The distribution such isolates in the environment their presence fish as result sewage contamination is not well-studied. Here we examined environmental samples from Mwanza city for ESBL-producing bacteria. From 196 sampled local markets, 26 (13.3 %) contained lactose-fermenting bacteria, while 39/73 (53.4 same area were ESBL producers. Antibiotic...
Extended-spectrum beta-lactamases (ESBL) mediating resistance to 3rd generation cephalosporins are a major public health issue. As food may be vehicle in the spread of ESLB-producing bacteria, study on occurrence cephalosporin-resistantu Escherichia coli was initiated. A total 404 ESBL-producing isolates were obtained from animal-derived samples (e.g., poultry products, pork, beef and raw milk) between 2011 2013. CTX-M-15 is most abundant enzyme E. causing human infections, this focusses...
A total of 17 Enterobacter-like isolates were obtained from blood during a septicaemia outbreak in neonatal unit, Tanzania, that could not be assigned based on phenotypic test to any existing Enterobacter species. Eight representative investigated detail. Fermentation characteristics, biochemical assays and fatty acid profiles for taxonomic analysis determined supplemented with information derived whole genome sequences. Phenotypic morphological tests revealed these Gram-stain-negative,...