A5025257212- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
- Pharmaceutical and Antibiotic Environmental Impacts
- Escherichia coli research studies
- Antibiotics Pharmacokinetics and Efficacy
- Salmonella and Campylobacter epidemiology
- Vibrio bacteria research studies
- Urinary Tract Infections Management
- Enterobacteriaceae and Cronobacter Research
- Antibiotic Use and Resistance
- Bacterial biofilms and quorum sensing
- Infections and bacterial resistance
- Pneumonia and Respiratory Infections
- Genomics and Phylogenetic Studies
- Probiotics and Fermented Foods
- Antimicrobial Resistance in Staphylococcus
- Gut microbiota and health
- Nosocomial Infections in ICU
- Bacteriophages and microbial interactions
- Bacillus and Francisella bacterial research
- Pneumocystis jirovecii pneumonia detection and treatment
- Tuberculosis Research and Epidemiology
- Neonatal and Maternal Infections
- Mycobacterium research and diagnosis
- Food Safety and Hygiene
Robert Koch Institute
2016-2025
SGS Germany GmbH (Germany)
2024-2025
The emergence and global spread of carbapenemase-producing Enterobacteriaceae is great concern to health services worldwide. These bacteria are often resistant all beta-lactam antibiotics frequently co-resistant most other antibiotics, leaving very few treatment options. epidemiology compounded by the diversity carbapenem-hydrolysing enzymes ability their genes between different bacterial species. Difficulties also encountered laboratories when trying detect carbapenemase production during...
To investigate the genetic environment of metallo-β-lactamase gene blaNDM-1 in an Acinetobacter baumannii isolated 2007 a German hospital. Antimicrobial susceptibility testing was performed and resistance genes were characterized by PCR amplification sequencing. Transferability β-lactam tested broth mating assays transformation plasmids. The background analysed primer walking. Typing A. strain repetitive extragenic palindromic sequence-based (rep-PCR) using DiversiLab system....
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is a potentially useful tool for the detection antimicrobial resistance, especially that conferred by β-lactamases. Here we describe modification previously reported MALDI-TOF MS meropenem hydrolysis assay. The modified method was validated on 108 carbapenemase-producing members Enterobacteriaceae, two NDM-1-producing Acinetobacter baumannii isolates, and 35 carbapenem-resistant enterobacteria...
The spread of Gram-negative bacteria with plasmid-borne extended-spectrum beta-lactamases (ESBLs) has become a worldwide problem. This study analysed total 366 ESBL-producing Enterobacteriaceae strains isolated from non-selected patient specimens at the university hospital Tübingen in period January 2003 to December 2007. Although overall ESBL rate was comparatively low (1.6 %), percentages Enterobacter spp. and Escherichia coli increased 0.8 0.5 %, respectively, 4.6 3.8 % In particular,...
To evaluate the possible occurrence of carbapenemase-producing Escherichia coli and Klebsiella spp. strains in domestic animals. Veterinary clinical E. (n = 1175) 136) isolates consecutively collected from livestock companion animals Germany June 2012 to October were screened for their susceptibility carbapenems using agar disc diffusion test. Carbapenemase genes characterized by PCR sequencing; conjugation assays performed. Carbapenemase-positive assigned phylogenetic lineages multilocus...
To determine the prevalence of extended-spectrum β-lactamase (ESBL) production in Enterobacteriaceae retail chicken meat Germany. A total 399 samples from nine supermarket chains, four organic food stores and one butcher's shop two geographically distinct regions (Berlin Greifswald) were screened for ESBL using selective agar. Phenotypic isolates tested blaTEM, blaCTX-M blaSHV genes PCR DNA sequencing. Antibiotic coresistances determined strain typing was performed PCR-based phylogenetic...
Escherichia (E.) coli producing extended-spectrum beta-lactamases (ESBLs) are an increasing problem for public health. The success of ESBLs may be due to spread ESBL-producing bacterial clones, transfer ESBL gene-carrying plasmids or exchange encoding genes on mobile elements. This makes it difficult identify transmission routes and sources bacteria. objectives this study were compare the distribution genotypic phenotypic properties E. isolates from different animal human collected in...
Resistance to 3rd-generation cephalosporins in Escherichia coli is mostly mediated by extended-spectrum beta-lactamases (ESBLs) or AmpC beta-lactamases. Besides overexpression of the species-specific chromosomal ampC gene, acquisition plasmid-encoded genes, e.g. blaCMY-2, has been described worldwide E. from humans and animals. To investigate a possible transmission blaCMY-2 along food production chain, we conducted next-generation sequencing (NGS)-based analysis 164 CMY-2-producing isolates...
We determined the presence of extended-spectrum-β-lactamase (ESBL)-producing Escherichia coli among 3,344 study participants from German community. Intestinal colonization was detected in 211 persons (6.3%), without significant differences different age groups. The majority (95.2%) isolates harbored CTX-M-type ESBL, with CTX-M-15 (46%) and CTX-M-1 (24.2%) as most common types. finding ESBL producers one isolate additionally producing carbapenemase OXA-244 indicates a risk dissemination...
By using whole genome sequence data we aimed at describing a population snapshot of carbapenemase-producing K. pneumoniae isolated from hospitalized patients in Germany between 2008 and 2014.We selected representative subset 107 clinical isolates possessing the four most prevalent carbapenemase types (KPC-2, KPC-3, OXA-48, NDM-1). Isolates were processed via illumina NGS. Data analysed different SNP-based mapping de-novo assembly approaches. Relevant information was extracted NGS (antibiotic...
ObjectivesTo investigate the clinical relevance and molecular epidemiology of extended-spectrum β-lactamase (ESBL)-producing Klebsiella species in animals.
ABSTRACT A comparison of a matrix-assisted laser desorption ionization–time flight mass spectrometric (MALDI-TOF MS) meropenem hydrolysis assay with the Carba NP test showed that both methods exhibited low sensitivity (approximately 76%), mainly due to false-negative results obtained OXA-48-type producers. The addition NH 4 HCO 3 reaction buffer for MALDI-TOF MS dramatically improved its (98%). Automatic interpretation assay, using MBT STAR-BL software, generally agreed after manual...
The spread of carbapenem-non-susceptible Klebsiella pneumoniae strains bearing different resistance determinants is a rising problem worldwide. Especially infections with KPC (Klebsiella carbapenemase) - producers are associated high mortality rates due to limited treatment options. Recent clinical studies KPC-blood stream revealed that colistin-based combination therapy carbapenem and/or tigecycline was significantly decreased when compared colistin monotherapy. However, it remains unclear...
One of the most demanding challenges in infection control is world-wide dissemination multidrug-resistant bacteria clinical settings. Especially increasing prevalence carbapenemase producing Gram-negative pathogens poses an urgent threat to public health, as these enzymes confer resistance almost all β lactam antibiotics including carbapenems. In this study, we report a prolonged nosocomial outbreak various NDM-1-producing Enterobacterales species due clonal spread and cross-species exchange...
In 2022, German surveillance systems observed rapidly increasing numbers of NDM-1- and NDM-1/OXA-48-producing Klebsiella pneumoniae, which may in part reflect recurring pre-pandemic trends. Among these cases, however, a presence Ukraine before diagnosis was frequently reported. Whole genome sequencing 200 isolates showed high prevalence sequence types ST147, ST307, ST395 ST23, including clusters corresponding to clonal dissemination suggesting onward transmission Germany. Screening isolation...
Background The war in Ukraine led to migration of Ukrainian people. Early 2022, several European national surveillance systems detected multidrug-resistant (MDR) bacteria related patients. Aim To investigate the genomic epidemiology New Delhi metallo-β-lactamase (NDM)-producing Providencia stuartii from patients among countries. Methods Whole-genome sequencing 66 isolates sampled 2022–2023 10 countries enabled whole-genome multilocus sequence typing (wgMLST), identification resistance genes,...
Klebsiella (K.) pneumoniae is a ubiquitous Gram-negative bacterium and common coloniser of animals humans. Today, K. one the most persistent nosocomial pathogens worldwide poses severe threat/burden to public health by causing urinary tract infections, pneumonia bloodstream infections. Affected infections are mainly immunocompromised individuals hospitalised patients. In recent years, new type has emerged associated with community-acquired such as pyogenic liver abscess in otherwise healthy...
This report focuses on the molecular characterization of 22 extended-spectrum β -lactamase-producing Escherichia coli isolates collected in a German university hospital during period 9 months 2006. Relationship analysis clinical was done via PFGE, multilocus sequence typing, plasmid profiling and additionally PCR for bla ESBL detection determination phylogroups. After conjugal transfer, isolation subsequent incompatibility groups were performed. Using one-primer walking, up to 3600 bp...
ABSTRACT Nine carbapenem-resistant Enterobacteriaceae isolates collected from eight patients in five German hospitals were investigated. Six produced the OXA-48 carbapenemase, and three OXA-162, which is a point mutant form of OXA-48. Both carbapenemase genes located on IncL/M-type conjugative plasmids. Insertion sequence IS 1999 (truncated or not by 1R ) was upstream bla OXA-162 all isolates. Pulsed-field gel electrophoresis typing indicated clonal transmission an OXA-48-producing...
Background The number of extended-spectrum beta-lactamase (ESBL) positive (+) Escherichia coli is increasing worldwide. In contrast with many other multidrug-resistant bacteria, it suspected that they predominantly spread within the community. objective this study was to assess factors associated community-acquired colonization ESBL E. coli. Methods We performed a matched case-control at Charité University Hospital Berlin between May 2011 and January 2012. Cases were defined as patients...