A5049518827- Antibiotic Resistance in Bacteria
- Enterobacteriaceae and Cronobacter Research
- Pneumonia and Respiratory Infections
- Escherichia coli research studies
- Bacterial biofilms and quorum sensing
- Bacterial Identification and Susceptibility Testing
- Antimicrobial Resistance in Staphylococcus
- Vibrio bacteria research studies
- Streptococcal Infections and Treatments
- Plant Pathogenic Bacteria Studies
- Antibiotics Pharmacokinetics and Efficacy
- Infections and bacterial resistance
- Pharmaceutical and Antibiotic Environmental Impacts
- Agricultural economics and policies
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Agriculture, Plant Science, Crop Management
- Antibiotic Use and Resistance
- Pneumocystis jirovecii pneumonia detection and treatment
- Tuberculosis Research and Epidemiology
- Urinary Tract Infections Management
- Virology and Viral Diseases
- Infective Endocarditis Diagnosis and Management
- Renewable energy and sustainable power systems
- Bacterial Infections and Vaccines
Narodowy Instytut Leków
2016-2025
National Institute of Public Health
2005-2019
Medical University of Warsaw
2012-2019
University of Groningen
2019
Hasanuddin University
2019
National Institute of Public Health
2016-2017
Plant Breeding and Acclimatization Institute - National Research Institute
1995-2008
Foundation for Polish Science
1999-2001
To perform the first multinational Enterobacter cloacae clonality study, using MLST scheme newly developed in Japan.The analysis included 195 rectal carriage E. isolates resistant to expanded-spectrum cephalosporins (ESCs), collected from patients 12 hospital units across Europe and Israel. All of were typed by PFGE 173 subjected MLST. ESC resistance was analysed phenotypically; genes encoding ESBLs carbapenemases identified PCR sequencing.MLST distinguished 88 STs, which correlated with...
ABSTRACT A comparison of a matrix-assisted laser desorption ionization–time flight mass spectrometric (MALDI-TOF MS) meropenem hydrolysis assay with the Carba NP test showed that both methods exhibited low sensitivity (approximately 76%), mainly due to false-negative results obtained OXA-48-type producers. The addition NH 4 HCO 3 reaction buffer for MALDI-TOF MS dramatically improved its (98%). Automatic interpretation assay, using MBT STAR-BL software, generally agreed after manual...
The objective of this study was to characterize New Delhi metallo-β-lactamase (NDM)-producing Enterobacteriaceae isolates reported in Poland 2012–14. Representative were typed by PFGE and MLST. NDM other β-lactamase genes amplified sequenced. Plasmids with blaNDM analysed nuclease S1 plus hybridization profiling, transfer assays PCR-based replicon typing. genetic context studied PCR mapping assays. Of 374 cases infection/colonization NDM-positive identified 2012–14, 370 40 hospitals, 10...
Background The war in Ukraine led to migration of Ukrainian people. Early 2022, several European national surveillance systems detected multidrug-resistant (MDR) bacteria related patients. Aim To investigate the genomic epidemiology New Delhi metallo-β-lactamase (NDM)-producing Providencia stuartii from patients among countries. Methods Whole-genome sequencing 66 isolates sampled 2022–2023 10 countries enabled whole-genome multilocus sequence typing (wgMLST), identification resistance genes,...
After the first report in May 2008, National Reference Center for Susceptibility Testing confirmed 113 cases of infection or colonization by KPC-producing members family Enterobacteriaceae Poland end 2009. The vast majority patients were found 18 hospitals; three diagnosed at outpatient clinics. Most institutions Warsaw area, including hospitals with highest numbers cases. When available, data on previous hospitalizations often indicated that these probable acquisition sites; one patient...
In February 2023, Escherichia coli sequence type (ST) 38 producing oxacillinase 244 (OXA-244-Ec ST38) was detected from three patients in a hospital western Poland. Overall, OXA-244-Ec ST38 colonised 13 wards between and June 2023. The outbreak investigated on site by an infection control team, the bacterial isolates were characterised microbiologically whole genome sequencing. We could not identify primary source of or reconstruct transmission sequence. some affected their groups linked...
The prospective project MOSAR was conducted in five rehabilitation units: the Berck Maritime Hôpital (Berck, France), Fondazione Santa Lucia (Rome, Italy), Guttmann Institute (GI; Barcelona, Spain), and Loewenstein Hospital Tel-Aviv Souraski Medical Center (TA) (Tel-Aviv, Israel). Patients were screened for carriage of Enterobacteriaceae resistant to expanded-spectrum cephalosporins (ESCs) from admission until discharge. aim this study characterize clonal structure, extended-spectrum...
ABSTRACT Poland's first Enterobacteriaceae isolate producing the New Delhi metallo-β-lactamase (NDM) was identified in August 2011. Escherichia coli sequence type ST410 NDM-1 cultured from a critically ill patient who had been transferred directly Congo. The bla gene carried by conjugative IncFII-type plasmid pMC-NDM (87,619 bp), which showed structural similarity to pGUE-NDM, earlier France an E. ST131 of Indian origin.
Despite a scarcity of data, before 2022 Ukraine was already considered high-prevalence country for carbapenemase-producing Enterobacterales (CPE), and the situation has dramatically worsened during full-scale war with Russia. The aim this study to analyse CPEs isolated in Poland from victims Ukraine. included 65 CPE isolates March till February 2023, recovered 36 Polish medical centres 57 patients arriving Ukraine, differing largely by age reason hospitalisation. All were sequenced MiSeq ten...
The international project MOSAR was conducted in five rehabilitation centers; patients were screened for rectal carriage of extended-spectrum β-lactamase (ESBL)-producing members the Enterobacteriaceae. Among 229 Klebsiella pneumoniae isolates, four clonal groups (CG) or complexes (CC) prevailed: CG17 France, CG101 Italy, CG15 Spain, and CC147 Israel. ESBLs, mainly CTX-Ms, produced by 226 isolates; three isolates expressed AmpC-like cephalosporinases. High genetic diversity K. populations...
Abstract Objectives The objective of this study was to perform a multinational survey patients' colonization by metallo-β-lactamase (MBL)-producing Enterobacteriaceae, including their molecular characterization. Methods Patients in 18 hospital units across Europe and Israel (n = 17 945) were screened between mid-2008 mid-2011. MBL-producing isolates typed PFGE MLST. MBL genes amplified sequenced within integrons. Plasmids with analysed nuclease S1 plus hybridization profiling, mating...
ABSTRACT In a 2008-2011 survey, 17,945 patients in 18 hospital units Europe and Israel were screened for carriage of Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae , resulting identification 124 positive patients. The isolates dominated by sequence type 258 (ST258) KPC-2 ST512 KPC-3, mainly from Greece Italy, respectively, whereas Israeli diverse species, clones, KPC variants. Various bla platforms observed, among which IncFII K -FIB plasmids with KPC-2/-3 genes the...
To analyse OXA-48 (OXA-48/181)-type carbapenemase-producing Enterobacteriaceae reported in Poland from 2013 until January 2017. Bacterial isolates were typed by PFGE and MLST. Genes coding for OXA-48/181 types other β-lactamases amplified sequenced. Mobile elements with blaOXA-48/181-like genes PCR mapped. blaOXA-48/181-carrying plasmids characterized nuclease S1-hybridization profiling, transfer assays PCR-based replicon typing, while the chromosomal location of was confirmed I-CeuI...
A population analysis of 103 multidrug-resistant Pseudomonas aeruginosa isolates from Croatian hospitals was performed. Twelve sequence types (STs) were identified, with a predominance international clones ST235 (serotype O11 [41%]), ST111 O12 [15%]), and ST132 O6 [11%]). Overexpression the natural AmpC cephalosporinase common (42%), but only few or produced VIM-1 VIM-2 metallo-β-lactamases PER-1 GES-7 extended-spectrum β-lactamases.
Abstract Objectives To characterize carbapenemase-producing isolates of the Klebsiella pneumoniae hypervirulent (hvKp) clone ST23 in Poland. Methods Fifteen K. were identified by Polish surveillance Enterobacterales. These comprised a cluster with KPC-2 + NDM-1 (n = 7), 1) or enzymes from one hospital 2018, and sporadic 1), VIM-1 OXA-48 3), recovered 2009 to 2019 different towns. The sequenced Illumina MiSeq, followed MinION for six representatives. Clonality, phylogeny, serotypes,...
We sequenced all nonduplicate 934 VIM/IMP carbapenemase-producing Enterobacterales (CPE) reported in Poland during 2006-2019 and found ≈40% of the isolates (n = 375) were Enterobacter spp. During study period, incidence those bacteria gradually grew nearly entire country. The major factor affecting increase was clonal spread several E. hormaechei lineages responsible for multiregional interregional outbreaks (≈64% isolates), representing mainly pandemic sequence type (ST) 90 or...
Abstract Unified strain taxonomies are crucial for fostering international communication in microbiological research and the epidemiological surveillance of bacterial pathogens. While multilocus sequence typing (MLST) has served as a foundation taxonomy two decades, whole genome sequencing enables more precise classifications significantly improves discriminatory resolution. The core genome-wide extension MLST (known cgMLST) thus holds great promise genotyping classification, but its...
The aim of this study was to investigate Polish clinical isolates Streptococcus pyogenes collected during a 7 year period using phenotypic and genotypic techniques.A total 816 S. recovered from 33 medical centres in Poland were tested for their susceptibility various antimicrobial agents. Erythromycin-resistant analysed by PFGE, multilocus sequence typing emm methods.The tetracycline resistance rate high (43%) among all strains. Ninety-eight (12%) resistant erythromycin. A low prevalence the...
We describe the introduction of NDM-1-producing Klebsiella pneumoniae ST147 and Escherichia coli ST410, OXA-48-producing K. ST101 strains to Poland by two patients transported country after hospitalisation in Tunisia. The had gunshot wounds following terrorist attack Bardo National Museum Tunis March 2015. Our report reinforces need for microbiological screening returning from travel on admission healthcare institutions, especially countries where carbapenemase-producing Enterobacteriaceae...