A5080402149- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Analytical Chemistry and Chromatography
- Phytochemicals and Antioxidant Activities
- Essential Oils and Antimicrobial Activity
- Protein Hydrolysis and Bioactive Peptides
- Antibiotic Resistance in Bacteria
- Microbial Inactivation Methods
- biodegradable polymer synthesis and properties
- Plant Virus Research Studies
- Cocoa and Sweet Potato Agronomy
- Free Radicals and Antioxidants
- Antioxidant Activity and Oxidative Stress
- Nutrition and Health Studies
- Process Optimization and Integration
- Advances in Cucurbitaceae Research
- Chromosomal and Genetic Variations
- Meat and Animal Product Quality
- Electrochemical sensors and biosensors
- Otolaryngology and Infectious Diseases
- Enterobacteriaceae and Cronobacter Research
- Solar-Powered Water Purification Methods
- Indoor Air Quality and Microbial Exposure
- Waste Management and Environmental Impact
Warsaw University of Life Sciences
2014-2025
Narodowy Instytut Leków
2017-2024
Institute of Food Science
2019
Institute of Industrial Organic Chemistry
2017
The availability of genome-sequencing and genome-editing techniques has increased the applicability innovative solutions, opening up revolutionary prospects for developments in horticultural plant breeding. Cucurbitaceae family is a group plants great importance horticulture due to their high nutritional economic value. These serve as important models elucidating principles development refining yield improvement strategies. While traditional breeding approaches have made significant...
Microbial lipids called a sustainable alternative to traditional vegetable oils invariably capture the attention of researchers. In this study, effect limiting inorganic phosphorus (KH2PO4) and nitrogen ((NH4)2SO4) sources in lipid-rich culture medium on efficiency cellular lipid biosynthesis by Y. lipolytica yeast has been investigated. batch cultures, carbon source was rapeseed waste post-frying oil (50 g/dm3). A significant relationship between concentration KH2PO4 amount accumulated...
To improve enzyme stability, the immobilization process is often applied. The choice of a support on which enzymes are adsorbed plays major role in enhancing biocatalysts’ properties. In this study, bio-based (i.e., chitosan, coffee grounds) and synthetic Lewatit VP OC 1600) supports were used lipases various microbial origins (yeast (Yarrowia lipolytica) mold (Aspergillus oryzae)). results confirmed that proteins had been surface selected carriers, but not all them revealed comparably high...
Yarrowia lipolytica is a robust yeast species that has gained significant attention as biofactory for various biotechnological applications and undoubtedly can be referred to hidden treasure trove due boasting diverse array of enzymes with wide-ranging in multiple industries, including biofuel production, food processing, biotechnology, pharmaceuticals. As the biotechnology field continues expand, Y. poised play pivotal role developing eco-friendly economically viable bioprocesses. Its...
Oil cakes are biomass wastes created by pressing oil from oilseeds. Their chemical composition (including high fat or protein content, a favorable fatty acid profile, and proportion of unsaturated acids) makes them valuable raw materials not only in animal feeding but increasingly gaining popularity biotechnological processes. This article examines the possibility valorizing using lipid fraction extracted their form two-pot biosynthesis process GDDL-a cyclic ester with creamy-peach aroma....
This study investigated the capabilities of Yarrowia lipolytica strains to grow in media with different hydrophobic wastes from meat industry. The yeast growth, cellular lipid accumulation, production lipases, and degree utilization liquid solid were studied shaken cultures organic inorganic nitrogen sources. effects type waste, initial concentration carbon source, strain, inoculum size two experimental sets using Latin Square 5 × design method. Post-frying rapeseed oil chicken frying was...
To analyse OXA-48 (OXA-48/181)-type carbapenemase-producing Enterobacteriaceae reported in Poland from 2013 until January 2017. Bacterial isolates were typed by PFGE and MLST. Genes coding for OXA-48/181 types other β-lactamases amplified sequenced. Mobile elements with blaOXA-48/181-like genes PCR mapped. blaOXA-48/181-carrying plasmids characterized nuclease S1-hybridization profiling, transfer assays PCR-based replicon typing, while the chromosomal location of was confirmed I-CeuI...
Coffee waste can be reused as matrices for enzyme immobilization, it contains various organic compounds able to adsorb catalytic proteins. In this study, spent coffee grounds were used a support in the immobilization process their native form and after being pretreated with hexane, ethanol, sulfuric acid solution, respectively. Microbial lipases from Aspergillus oryzae, Thermomyces lanuginosus, Rhizomucor miehei adsorbed on carrier, reference, abovementioned also immobilized synthetic matrix...
The aim of the study was to evaluate possibility utilize a fish waste oil issued from industrial smoking process in nitrogen-limited Yarrowia lipolytica yeast batch cultures. carbon source utilized by and stimulated single cell production via an ex novo pathway. biomass contained lipids up 0.227 g/g d.m.. Independently culture conditions, high contents very long chain fatty acids were quantified including docosahexaenoic (DHA), eicosapentaenoic acid (EPA), eicosenic erucic acids. pH...
Plant and animal oils fats currently dominate the edible oil market, but a new sustainable alternative of lipids from single-celled organisms has become advantageous in human nutrition pharmacy. Single-cell (SCOs) are biosynthesized accumulated lipid bodies oleaginous species bacteria, yeasts, molds, algae. The review investigated SCOs’ composition, with detailed described beneficial impact medicine, cosmetics, pharmacy, nutrition. Although microbial been known for more than 100 years, it...
Single cell oil (SCO) is the lipid accumulated in cells of oleaginous microorganisms. Cellular lipids can be synthesized two different pathways: de novo by metabolizing hydrophilic substrates and ex fermenting hydrophobic substrates. The aim study was to evaluate effect carbon source (glucose olive oil) culture medium on course microbial accumulation Y.lipolytica cells. level selected gene expression real time quantitative PCR method investigated. significant increase POX2 encoding acyl-CoA...
The aim of the study was to compare effect substituent and its position in aromatic ring on antioxidant activity hexanoic acid esters obtained reactions catalyzed by immobilized lipase B from Candida antarctica. 4-Hydroxybenzyl hexanoate, 2-hydroxybenzyl 4-methoxybenzyl vanillyl hexanoate were with conversion yields 50 80%. synthesized esters, their alcohol precursors BHT (Butylated HydroxyToluene) compared DPPH (2,2-diphenyl-1-picrylhydrazyl), CUPRAC (cupric ion reducing capacity), CBA...
Abstract The review aims to summarize the current knowledge on possibility of using non-conventional yeast species Yarrowia lipolytica in treatment and upgradation industry wastes. Importantly Y. is argued as generally recognized safe species, what indicates high application potential reviewed technologies. Special emphasis paper was given microbial processing food wastes, including fish animals’ wastes utilization. -based waste cooking oil or oil-bearing plants wastewaters, such palm mill...
Lipases are versatile catalysts widely used in industrial biotransformations and laboratory-scale developed reactions with potential. Despite the fact that lipase B from Candida antarctica (CALB) is one of most lipolytic enzymes, its substrate specificity still poorly understood. One observed trend carried out carboxylic acids containing a double bond less efficient on average. Here, we have utilized combination vitro silico techniques, to better understand negative impact CALB-mediated...
Yarrowia lipolytica is one of the most studied non-conventional forms yeast, exhibiting a high secretory capacity and producing many industrially important valuable metabolites. The yeast conceals great biotechnological potential to synthesize organic acids, sweeteners, microbial oil, or fragrances. vast majority bioprocesses are carried out in bioreactors, where suitable culture conditions provided. In current study, effect agitation speed (200–600 rpm) air flow rate (0.0375–2.0 dm3/(dm3 ×...
There is a growing demand for vegan products and plant-based food when dealing with the impact of livestock on climate crisis. The aim this study was to develop formulation analogue mold-ripened cheese. Were investigated following plant materials: cashews, pistachios, soy flour, chickpea pea protein, pumpkin hemp spirulina powder. Plant matrices were fermented lactic acid bacteria (LAB) starter cultures cheese mold species Geotrichum candidum Penicillium camemberti. All microorganisms’...
Artificial intelligence (AI) can revolutionize agriculture by enhancing genomic research and promoting sustainable crop improvement. AI systems integrate machine learning (ML) deep (DL) with big data to identify complex patterns relationships analyzing vast genomic, phenotypic, environmental datasets. This capability accelerates breeding cycles, improves predictive accuracy, supports the development of climate-resilient, high-yielding varieties. Applications such as precision agriculture,...