A5077732489- Antibiotic Resistance in Bacteria
- Streptococcal Infections and Treatments
- Antimicrobial Resistance in Staphylococcus
- Infective Endocarditis Diagnosis and Management
- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- Pharmaceutical and Antibiotic Environmental Impacts
- Enterobacteriaceae and Cronobacter Research
- Bacterial Identification and Susceptibility Testing
- Pneumonia and Respiratory Infections
- Biosensors and Analytical Detection
- Bacteriophages and microbial interactions
- Immunodeficiency and Autoimmune Disorders
- Plant Pathogenic Bacteria Studies
- Allelopathy and phytotoxic interactions
- Infectious Aortic and Vascular Conditions
- Microbial infections and disease research
- Escherichia coli research studies
- Ferroptosis and cancer prognosis
- Antibiotic Use and Resistance
- Infections and bacterial resistance
- Bacterial biofilms and quorum sensing
- Vibrio bacteria research studies
- Probiotics and Fermented Foods
- Viral gastroenteritis research and epidemiology
Medical University of Lublin
2023-2024
Narodowy Instytut Leków
2013-2024
National Institute of Public Health
2008
The immune system protects against pathogens, and its dysfunction leads to primary secondary immunodeficiencies, increasing infection susceptibility. Epstein-Barr virus (EBV) reactivation is linked homeostasis disorders, particularly in common variable immunodeficiency (CVID) chronic lymphocytic leukemia (CLL). Toll-like receptor (TLR) pathways play a crucial role innate immunity, their deregulation may contribute dysfunction. This study aimed assess the impact of EBV on homeostasis,...
To analyse OXA-48 (OXA-48/181)-type carbapenemase-producing Enterobacteriaceae reported in Poland from 2013 until January 2017. Bacterial isolates were typed by PFGE and MLST. Genes coding for OXA-48/181 types other β-lactamases amplified sequenced. Mobile elements with blaOXA-48/181-like genes PCR mapped. blaOXA-48/181-carrying plasmids characterized nuclease S1-hybridization profiling, transfer assays PCR-based replicon typing, while the chromosomal location of was confirmed I-CeuI...
Phage vB_SauP_phiAGO1.3 (phiAGO1.3) is a polyvalent Staphylococcus lytic podovirus with 17.6-kb genome (Gozdek et al., 2018). It can infect most of the aureus human isolates dominant clonal complexes. We show that major factor contributing to wide host range phiAGO1.3 lack or sparcity target sites for certain restriction-modification systems types I and II in its genome. requires adsorption β-O-GlcNAcylated cell wall teichoic acid, which also essential expression methicillin resistance....
The European epidemiology of MRSA is changing with the emergence community-associated (CA-MRSA) and livestock-associated (LA-MRSA). In this study, we investigated molecular during 2 years in 13 ICUs France, Greece, Italy, Latvia, Luxemburg, Portugal, Slovenia Spain.Surveillance cultures for from nose wounds were obtained on admission twice weekly all patients admitted to an ICU ≥3 days. first isolate per patient was genotyped a central laboratory by MLST, spa typing, agr typing SCCmec...
Viscum album L. (European mistletoe), a member of the Santalaceae, is hemiparasitic, evergreen shrub growing on deciduous and coniferous trees. In traditional folk medicine, mistletoe was used for treatment central nervous system disorders such as epilepsy, hysteria, insomnia, excitability, neuralgia, headache, dizziness fatigue. However, relatively little known its neuropharmacological activity.The aim present study to evaluate effect with aqueous hydroethanolic extracts from parasitizing...
Background Neisseria meningitidis is a leading etiologic agent of severe invasive disease. The objective the study was to characterise meningococcal disease (IMD) epidemiology in Poland during last decade, based on laboratory confirmed cases. Methods encompassed all meningococci collected between 2002 and 2011 National Reference Centre for Bacterial Meningitis. isolates were re-identified characterised by susceptibility testing, MLST analysis, porA fetA sequencing. A PCR technique used...
In 2015 and 2016 Poland recorded rapid proliferation of New Delhi MBL (NDM)-producing Enterobacterales, with at least 470 1780 cases, respectively. We addressed the roles Klebsiella pneumoniae ST11 NDM-1 outbreak genotype, already spreading in 2012-14, newly imported organisms this increase.The study included 2136 NDM-positive isolates identified between April December 2016, following transfer patients K. ST147 from Tunisia to Warsaw March 2015. The were screened by PCR mapping for variants...
Periodontitis is a common oral condition that can have significant impact on the overall health of body. In recent years, attention has been paid to potential relationships between periodontitis and various hematological disorders. This publication aims present information available in literature this relationship, focusing examples red blood cell disorders (such as aplastic anemia sickle anemia) white cyclic neutropenia, maladaptive trained immunity, clonal hematopoiesis, leukemia, multiple...
Rapid identification of SARS-CoV-2 variants is essential for epidemiological surveillance. RT-qPCR-based variant differentiation tests can be used to quickly screen large sets samples relevant concern/interest; this study was conducted on specimens collected at 11 centers located in Poland during routine diagnostics between August 2020 and December 2021. A total 1096 (with CT < 30) were screened Alpha, Beta, Delta, Kappa Omicron using commercial assays targeting repeat mutation sites....
One of the tools to contain SARS-CoV-2 pandemic was increase number performed tests and improve access diagnostics. To this effect, mobile collection sites (MCSs) were established. This study on samples collected at MCS between November 2020 March 2021. We aimed confirm/exclude SARS-CoV-2, differentiate variants, detect other respiratory pathogens. viruses identified by RT-qPCRs. A total 876 (46.35%) positive specimens in diagnostic identified. The wild-type variant determined 667 (76.14%)...
Accurate and rapid identification of COVID-19 is critical for effective patient treatment disease outcomes, as well the prevention SARS-CoV-2 transmission. Rapid antigen tests (RATs) identifying are simpler, faster less expensive than molecular assays. Any new product to be considered a medical device subject evaluation data analysis verify in vitro diagnostic ability achieve its intended purpose. Clinical validation such test prerequisite before clinical application. This study was on adult...
The present investigation was undertaken to assess the proportion of methicillin-resistant Staphylococcus aureus (MRSA) strains among hospital-acquired isolates and determine clones MRSA currently circulating in Poland by using a number molecular techniques. Between January May 2005, methicillin resistance investigated total 915 S. collected from 39 hospitals. A 208 (22.7%) were positive for mecA gene PCR. characterization carried out multiple-locus variable-number tandem repeat...
Streptococcus anginosus, intermedius and constellatus form a group of related streptococcal species, namely the Anginosus Group (SAG). The group, previously called "milleri" had been rarely described until 1980/1990 as source infections. Nowadays SAG bacteria are often pathogens causing predominantly purulent number infections is highly underestimated, strains classified in microbiology laboratory less virulent "viridans streptococci" Epidemiological situation regarding Poland has...
COVID-19 was initially reported in China at the end of 2019 and soon thereafter, March 2020, WHO declared it a pandemic. Until October 2021, over 240 million cases were recorded, with 4.9 mln deaths. In order to stop spread this disease, is crucial monitor detect any infected person. The etiologic agent novel coronavirus called SARS-CoV-2. gold standard for detection virus RT-qPCR method. This study evaluated two RNA extraction methods four commercial assays routinely used diagnostic...
Thirty-eight AmpC-producing Klebsiella pneumoniae isolates identified from January to October 2006 in a large teaching hospital the Czech Republic were analyzed. The AmpC cephalosporinase was as DHA-1, encoded by plasmid-located complex class 1 integron, previously observed K. isolate Parisian region. DHA-1 expression inducible, and although two with higher resistance, induction effect masked at phenotypic level. All of belonged international clone sequence type 11, split into disseminated...
For many years, Streptococcus anginosus has been considered a commensal colonizing the oral cavity, as well gastrointestinal and genitourinary tracts. However, recent epidemiological clinical data designate this bacterium an emerging opportunistic pathogen. Despite reported pathogenicity of S. anginosus, molecular mechanism underpinning its virulence is poorly described. Therefore, our goal was to develop optimize efficient simple infection models that can be applied examine study...
Streptococcus pyogenes (group A Streptococcus, GAS) is a major human pathogen and causes every year over 600 millions upper respiratory tract onfections worldwide. Untreated or repeated infections may lead to post-infectional sequelae such as rheumatic heart disease, cause of GAS-mediated mortality. There no comprehensive, longitudinal analysis the M type distribution strains isolated in Poland. Single reports describe rather their antibiotic resistance patterns focus on invasive isolates....
We compared the susceptibility to viral infection of splenocytes, isolated from young versus old CBA mice, and evaluated antiviral actions lactoferrin in splenocytes infected with Encephalomyocarditis virus (EMCV). Recombinant mouse (rmLF) bovine (bLF) were used. There no differences EMCV studied age categories. Both types lactoferrins protective mice. The study confirmed undisturbed resistance mice infection. action homologous was demonstrated for first time.
Sixty-five colistin-resistant Enterobacterales isolates recovered from different clinical specimens were analyzed. The strains collected in 12 hospitals all over Poland within a period of nine months. Strains analyzed for eight genes the mcr family. presence mcr-1 gene was detected three Escherichia coli strains. 45/65 identified as ESBL producers. CTX-M-1-like enzymes most common ESBLs (n = 40). One E. and seven Klebsiella pneumoniae produced carbapenemases, with NDM being by five isolates....
Abstract Background The antibiotic resistance determinants and associated mobile genetic elements (MGEs) were detected among Streptococcus pyogenes (group A streptococci [GAS]) clinical isolates of an M77 serotype collected in Poland between 2003 2017. Methods genomes 136 GAS sequenced using short- selected with long-read approach; whole genome sequences analyzed to determine the context macrolide determinants. Results analysed strains from in- outpatients. Sequencing data analysis revealed...