A5043798404- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Microbial Community Ecology and Physiology
- Bacterial Infections and Vaccines
- Infective Endocarditis Diagnosis and Management
- Streptococcal Infections and Treatments
- Pneumonia and Respiratory Infections
- DNA Repair Mechanisms
- Plant Pathogenic Bacteria Studies
- Protist diversity and phylogeny
- Geology and Paleoclimatology Research
- Potato Plant Research
- Antibiotic Resistance in Bacteria
- Carcinogens and Genotoxicity Assessment
- Plant Pathogens and Resistance
- Water Treatment and Disinfection
- Methane Hydrates and Related Phenomena
- Bacterial biofilms and quorum sensing
- Polar Research and Ecology
- Plant Virus Research Studies
- Antimicrobial Resistance in Staphylococcus
- Microbial infections and disease research
- Pneumocystis jirovecii pneumonia detection and treatment
Institute of Biochemistry and Biophysics, Polish Academy of Sciences
2013-2024
Phage vB_SauP_phiAGO1.3 (phiAGO1.3) is a polyvalent Staphylococcus lytic podovirus with 17.6-kb genome (Gozdek et al., 2018). It can infect most of the aureus human isolates dominant clonal complexes. We show that major factor contributing to wide host range phiAGO1.3 lack or sparcity target sites for certain restriction-modification systems types I and II in its genome. requires adsorption β-O-GlcNAcylated cell wall teichoic acid, which also essential expression methicillin resistance....
The complex cell wall structure of algae often precludes efficient extraction their genetic material. purpose this study was to design a next-generation sequencing-suitable DNA isolation method for unicellular, achlorophyllous, yeast-like microalgae the genus Prototheca, only known plant pathogens both humans and animals. effectiveness newly proposed scheme compared with five other, previously described methods, commonly used from plants and/or yeasts, available either as...
In the majority of bacterial species, tripartite ParAB-parS system, composed an ATPase (ParA), a DNA-binding protein (ParB), and its target parS sequence(s), assists in chromosome partitioning. ParB forms large nucleoprotein complexes at parS(s), located vicinity origin chromosomal replication (oriC), which after are subsequently positioned by ParA cell poles. Remarkably, participate not only segregation but through interactions with various cellular partners they also involved other...
Adélie penguins (Pygoscelis adeliae) are the most numerous flightless bird group breeding in coastal areas of Maritime and Continental Antarctica. Their activity leaves a mark on land form large guano deposits. This is an important nutrient source for terrestrial habitats ice-free Antarctic areas, notably by being ammonia vapors which feed surrounding grass, lichen algae communities. Although investigated researchers, fate guano-associated microbial community its role decomposition processes...
Alkylating agents introduce cytotoxic and/or mutagenic lesions to DNA bases leading induction of adaptive (Ada) response, a mechanism protecting cells against deleterious effects environmental chemicals. In Escherichia coli, the Ada response involves expression four genes: ada, alkA, alkB, and aidB. Pseudomonas putida, organization regulon is different, raising questions regarding regulation gene expression. The aim presented studies was analyze role AlkA glycosylase AlkB dioxygenase in P....
The introduction of pneumococcal conjugate vaccines (PCV) into the national immunization programs (NIPs) has significantly reduced number infections. However, infections caused by isolates non-vaccine serotypes (NVT) started spreading shortly thereafter and strains NVT 19A have become main cause invasive disease burden worldwide. aim study was to characterize serotype pneumococci GPSC1/CC320 circulating in Poland before PCV Polish NIP 2017 compare them from other countries where PCVs were...
Abstract This study provides a deep modern insight into the phylogenetic diversity among bacterial consortia found in working and nonworking high‐methane natural gas pipelines located Poland. The pipeline was characterized by lower biodiversity (140–154 genera from 22 to 23 classes, depending on source of debris) comparison off‐gas (169 classes). sediment recovered contained mostly DNA identified as belonging phylum Firmicutes (66.4%–45.9% operational taxonomic units [OTUs]), predominantly...
Pseudomonas aeruginosa, a human opportunistic pathogen, is common cause of nosocomial infections. Its ability to survive under different conditions relies on complex regulatory network engaging transcriptional regulators controlling metabolic pathways and capabilities efficiently use the available resources. P. aeruginosa PA3973 encodes an uncharacterized TetR family regulator. In this study, we applied transcriptome profiling (RNA-seq), genome-wide identification binding sites using...
P1 is a model, temperate bacteriophage of the 94 kb genome. It can lysogenize representatives Enterobacterales order. In lysogens, it maintained as plasmid. We tested interactions with biocontrol P. agglomerans L15 strain to explore utility in genome engineering. A derivative carrying Tn 9 (cm R ) transposon could transfer plasmid from Escherichia coli cells. The cells infected this formed chloramphenicol-resistant colonies. They grow liquid medium chloramphenicol after adaptation and did...
Abstract Background The antibiotic resistance determinants and associated mobile genetic elements (MGEs) were detected among Streptococcus pyogenes (group A streptococci [GAS]) clinical isolates of an M77 serotype collected in Poland between 2003 2017. Methods genomes 136 GAS sequenced using short- selected with long-read approach; whole genome sequences analyzed to determine the context macrolide determinants. Results analysed strains from in- outpatients. Sequencing data analysis revealed...