A5010272337- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Infective Endocarditis Diagnosis and Management
- Bacterial biofilms and quorum sensing
- Streptococcal Infections and Treatments
- Pneumonia and Respiratory Infections
- Mechanical Circulatory Support Devices
- Antibiotic Resistance in Bacteria
- Antibiotic Use and Resistance
- Orthopedic Infections and Treatments
- Respiratory viral infections research
- Antibiotics Pharmacokinetics and Efficacy
- Pneumocystis jirovecii pneumonia detection and treatment
- Immune Response and Inflammation
- Microbial infections and disease research
- Cardiac Arrest and Resuscitation
- Biochemical and Structural Characterization
- Cardiac Structural Anomalies and Repair
- Cardiac Valve Diseases and Treatments
- Antimicrobial Peptides and Activities
- Microbial Metabolism and Applications
- Nitric Oxide and Endothelin Effects
- Nosocomial Infections in ICU
- Adenosine and Purinergic Signaling
- Amoebic Infections and Treatments
Erasmus MC
2011-2025
Erasmus University Rotterdam
2008-2023
Erasmus MC - Sophia Children’s Hospital
2010
Radboud University Nijmegen
2004
Radboud University Medical Center
2004
Background. Persistent nasal carriers have an increased risk of Staphylococcus aureus infection, whereas intermittent and noncarriers share the same low risk. This study was performed to provide additional insight into staphylococcal carriage types. Methods. Fifty‐one volunteers who had been decolonized with mupirocin treatment whose state known were colonized artificially a mixture S. strains, intranasal survival compared between groups. Antistaphylococcal antibody levels also among 83...
Persistent carriers have a higher risk of Staphylococcus aureus infections than noncarriers but lower bacteremia-related death. Here, the role played by anti-staphylococcal antibodies was studied.Serum samples from 15 persistent and 19 were analyzed for immunoglobulin (Ig) G, IgA, IgM binding to S. antigens, means Luminex technology. Nasal secretions serum obtained after 6 months also analyzed.Median IgG levels significantly in toxic shock syndrome toxin (TSST)-1 (median fluorescence...
Background The war in Ukraine led to migration of Ukrainian people. Early 2022, several European national surveillance systems detected multidrug-resistant (MDR) bacteria related patients. Aim To investigate the genomic epidemiology New Delhi metallo-β-lactamase (NDM)-producing Providencia stuartii from patients among countries. Methods Whole-genome sequencing 66 isolates sampled 2022–2023 10 countries enabled whole-genome multilocus sequence typing (wgMLST), identification resistance genes,...
Attempts to develop an efficient anti-staphylococcal vaccine in humans have so far been unsuccessful. Therefore, more knowledge of the antigens that are expressed by Staphylococcus aureus human blood and induce immune response patients is required. In this study we further characterize serial levels IgG IgA antibodies against 56 staphylococcal multiple serum samples 21 with a S. bacteremia, compare peak between 30 non-infected controls, analyze expression 3626 genes two genetically distinct...
Since March 2022, there has been an emergence of multidrug-resistant organisms (MDRO) in the Netherlands patients originating from Ukraine (58 patients, 75 isolates). For about half these recent hospitalisation was reported. Genomic surveillance revealed that majority MDRO represent globally spread epidemic lineages and 60% contain New Delhi metallo-β-lactamase (NDM) genes. Professionals should be aware increase such associated with migration medical evacuation people Ukraine.
Expanding knowledge on the humoral immune response in Staphylococcus aureus-infected patients is a mandatory step development of vaccines and immunotherapies. Here, we present novel insights into antibody responses following S. aureus bacteremia. Fifteen bacteremic were followed extensively from diagnosis onwards (median 29 days, range 9-74). strains 3, 1-6) serial serum samples 16, 6-27) collected. Strains genotyped by pulsed-field gel electrophoresis (PFGE) genes encoding 19 staphylococcal...
Toxins are important Staphylococcus aureus virulence factors, but little is known about their immunogenicity during infection. Here, additional insight generated. Serum samples from 206 S. aureus-infected patients and 201 hospital-admitted control subjects were analyzed for immunoglobulin (Ig) G binding to 20 toxins, using flow-cytometry based technology. Antibody levels associated with polymerase chain reaction-defined presence of toxin genes in homologous isolates. IgG directed exfoliative...
ABSTRACT The currently available pneumococcal vaccines do not protect against all serotypes of Streptococcus pneumoniae . A shift toward nonvaccine causing colonization and invasive disease has occurred, studies on protein-based have been undertaken. We assessed the association between specific antibodies virulence proteins respiratory tract infections (RTIs). Additionally, we extent to which induces a humoral immune response. Nasopharyngeal swabs collected from children at 1.5, 6, 14, 24...
ABSTRACT Since March 2022, an increase was observed in multidrug-resistant microorganisms (MDRO), associated with the hospital transfer of Ukrainian patients. The goal to collect phenotypic susceptibility data and assess clinical implications. Carbapenemase-producing Enterobacterales (CPE, n = 96), Pseudomonas aeruginosa (CPPA, 20), carbapenem-resistant Acinetobacter baumannii–calcoaceticus (CRAB, 6) from patients were obtained December 2022 Dutch MDRO surveillance. Antimicrobial testing...
Antimicrobial lead time (ALT) is the from antimicrobial order to administration, an understudied parameter. This study aims determine feasibility of retrieving ALT, differences in ALT for different infectious diseases and association with length stay (LoS) establish value this parameter as potential new process or quality indicator (QI). In a retrospective tertiary care hospital Netherlands, adult hospitalized patients treated infection were included over 20-month period. was calculated data...
To study the characteristics of patients with Propionibacterium acnes prosthetic valve endocarditis (PVE) who required surgery.A single-centre retrospective cohort was conducted during a 7-year period. Patients definite infective P. endocarditis, according to modified Duke criteria, were included. An extended culture protocol applied. Information on medical health status, surgery, antibiotic treatment and mortality obtained.Thirteen fulfilled criteria for (0.53% 2466 replacement in period)....
Biofilm-associated infections with Staphylococcus aureus are difficult to treat even after administration of antibiotics that according the standard susceptibility assays effective. Currently, used in clinical laboratories determine sensitivity S . towards not representing behaviour biofilm-associated , since these performed on planktonic bacteria. In research settings, microcalorimetry has been for antibiotic studies. Therefore, this study we investigated if can use isothermal monitor...
Autologous vaccines (short: autovaccines) have been used since the beginning of 20th century to treat chronic staphylococcal infections, but their mechanisms action are still obscure. This prospective pilot study involved four patients with furunculosis who were vaccinated autologous formalin-killed Staphylococcus aureus cells. Vaccines individually prepared from infecting S. strain and repeatedly injected subcutaneously in increasing doses over several months. We characterized virulence...
Summary Infective endocarditis (IE) is the cardiac disease with highest rates of mortality. New biomarkers that are able to identify patients at risk for death required improve patient management and outcome. This study aims investigate if cytokines, chemokines growth factors measured IE diagnosis can predict Patients definite IE, according Duke’s modified criteria, were included. Using high-performance Luminex assay, 27 different analyzed. Machine learning techniques used prediction...