A5074409519- Virology and Viral Diseases
- Respiratory viral infections research
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Viral Infections and Vectors
- SARS-CoV-2 and COVID-19 Research
- Herpesvirus Infections and Treatments
- Viral gastroenteritis research and epidemiology
- Animal Disease Management and Epidemiology
- Influenza Virus Research Studies
- Viral Infections and Immunology Research
- Mosquito-borne diseases and control
- interferon and immune responses
- Pneumonia and Respiratory Infections
- Hepatitis Viruses Studies and Epidemiology
- Parvovirus B19 Infection Studies
- Bacteriophages and microbial interactions
- Vector-Borne Animal Diseases
- Neonatal Respiratory Health Research
- Microbial infections and disease research
- Vaccine Coverage and Hesitancy
- Gene expression and cancer classification
- Tracheal and airway disorders
- Rabies epidemiology and control
- COVID-19 Clinical Research Studies
University of Veterinary Medicine Hannover, Foundation
2015-2025
Artemis One Health Research Foundation
2017-2018
Utrecht University
2018
Boston University
2012-2017
University of Veterinary Medicine
2017
University of Wuppertal
2017
Bernhard Nocht Institute for Tropical Medicine
2017
Belfast Health and Social Care Trust
2014
Queen's University Belfast
2005-2011
Erasmus University Rotterdam
2010-2011
Measles virus (MV) is hypothesized to enter the host by infecting epithelial cells of respiratory tract, followed viremia mediated infected monocytes. However, neither these cell types express signaling lymphocyte activation molecule (CD150), which has been identified as receptor for wild-type MV. We have rhesus and cynomolgus macaques with a recombinant MV strain expressing enhanced green fluorescent protein (EGFP); thus bringing together optimal animal model measles that can be detected...
Measles virus (MV) is highly infectious, and has long been thought to enter the host by infecting epithelial cells of respiratory tract. However, do not express signaling lymphocyte activation molecule (CD150), which high-affinity cellular receptor for wild-type MV strains. We have generated a new recombinant strain expressing enhanced green fluorescent protein (EGFP), based on genotype B3 isolate from Khartoum, Sudan (KS). Cynomolgus macaques were infected with high dose rMVKSEGFP aerosol...
Abstract Currently, infections with SARS-Coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 pandemic, are responsible for substantial morbidity and mortality worldwide. Older adults subjects > 60 years age account 95% over one million fatal cases reported to date. It is unclear why in this group SARS-CoV-2 infection causes more severe disease than young adults. We hypothesized that differences cross-reactive cellular immunity induced after human coronaviruses (HCoVs), like OC43...
Recent reports documenting sporadic infections in carnivorous mammals worldwide with highly pathogenic avian influenza virus (HPAIV) H5N1 clade 2.3.4.4b have raised concerns about the potential risk of adaptation to sustained transmission mammals, including humans. We report infection two grey seals (Halichoerus grypus) from coastal waters The Netherlands and Germany December 2022 February 2023, respectively. Histological immunohistochemical investigations showed both animals a...
The global increase in measles vaccination has resulted a significant reduction of mortality. standard route administration for the live-attenuated virus (MV) vaccine is subcutaneous injection, although alternative needle-free routes, including aerosol delivery, are under investigation. In vitro, attenuated MV much wider tropism than clinical isolates, as it can use both CD46 and CD150 cellular receptors. To compare vivo pathogenic MV, we infected cynomolgus macaques with or recombinant...
Abstract Canine circoviruses (CanineCV’s), belonging to the genus Circovirus of Circoviridae family, were detected by next generation sequencing in samples from Thai dogs with respiratory symptoms. Genetic characterization and phylogenetic analysis nearly complete CanineCV genomes suggested that natural recombination had occurred among different lineages CanineCV’s. Similarity plot bootscaning analyses indicated American Chinese viruses served as major minor parental viruses, respectively....
ABSTRACT Measles virus (MV), one of the most contagious viruses infecting humans, causes a systemic infection leading to fever, immune suppression, and characteristic maculopapular rash. However, specific mechanism or mechanisms responsible for spread MV into respiratory epithelium in late stages disease are unknown. Here we show crucial role PVRL4 mediating from epithelial cells by generating “blind” recombinant wild-type developing novel vitro coculture model B with primary differentiated...
Although live-attenuated measles virus (MV) vaccines have been used successfully for over 50 years, the target cells that sustain replication in vivo are still unknown. We generated a reverse genetics system MV vaccine strain Edmonston-Zagreb (EZ), allowing recovery of recombinant (r)MV(EZ). Three viruses were contained open reading frame encoding enhanced green fluorescent protein (EGFP) within an additional transcriptional unit (ATU) at various positions genome. rMV(EZ)EGFP(1),...
Pestiviruses typically infect members of the order Artiodactyla, including ruminants and pigs, although putative rat bat pestiviruses have also been described. In present study, we identified characterized an evolutionary divergent pestivirus in toothed whale species, harbour porpoise (Phocoena phocoena). We tentatively named virus Phocoena (PhoPeV). PhoPeV displays a typical genome organization except for unique absence Npro, N-terminal autoprotease that targets innate host immune response....
Characterization of human measles cases is essential in order to better assess the data generated model systems morbillivirus infection. To this end, we collected formalin-fixed tissue samples from 23 natural different areas world and phases disease ranging prodromal acute a persistent infection an immunocompromised subject. We show that vast majority virus (MV)-infected cells epithelia were intraepithelial immune were, most cases, positive for CD11c myeloid cell marker. Small numbers...
Bocaviruses are small nonenveloped DNA viruses belonging to the Bocaparvovirus genus of Parvoviridae family and have been linked both respiratory enteric disease in humans animals. To date, 3 bocaviruses, canine bocaviruses 1 (CBoV-1–3), shown affect dogs with different manifestations reported for infected We used next-generation sequencing identify a novel strain CBoV-2 (CBoV TH-2016) litter puppies that died Thailand from acute dyspnea hemoptysis, which no causal pathogen could be...
Abstract Respiratory syncytial virus (RSV) is one of the leading causes respiratory tract infection in children, immunocompromised individuals and older adults. Vaccines have recently been approved for use adults although further efforts to develop suitable interventions children are ongoing, there limited animal models RSV infection. For preclinical efficacy testing prophylactic therapeutic treatments cotton rat ferret can be used. However, these expensive, difficult source house, often...
Abstract The discovery of several novel hepatovirus species in marine and terrestrial mammals has expanded the recognised members genus Hepatovirus provided better understanding on evolutionary origins human A (HAV). Using high throughput sequencing we detected a seal (SealHAV_NL/PV/21), liver tissue deceased harbor ( Phoca vitulina ) originating from Dutch North Sea coast. RT-PCR screening samples 88 seals 12 grey Halichoerus grypus same region identified nine juvenile which minor sequence...
Respiratory syncytial virus (RSV) is an important cause of acute respiratory disease in infants, immunocompromised subjects and the elderly. However, it unclear why most primary RSV infections are associated with relatively mild symptoms, whereas some result severe lower tract bronchiolitis. Since hospitalization has been bacterial co-infections, we have tested if Toll-like receptor (TLR) agonists influence RSV-A2-GFP infection human cells or cell lines. The synthetic lipopeptide...
The lymphotropic and myelotropic nature of wild-type measles virus (wt-MV) is well recognized, with dendritic cells lymphocytes expressing the MV receptor CD150 mediating systemic spread virus. Infection respiratory epithelial has long been considered crucial for entry into body. However, lack detectable on these raises issue their importance in pathogenesis measles. This study utilized a combination vitro, ex vivo model systems to characterize susceptibility wt-MV proven pathogenicity. Low...
ABSTRACT Measles virus (MV) is being considered for global eradication, which would likely reduce compliance with MV vaccination. As a result, children will grow up without MV-specific immunity, creating potential niche closely related animal morbilliviruses such as canine distemper (CDV). Natural CDV infection causing clinical signs has never been reported in humans, but recent outbreaks captive macaques have shown that can cause disease primates. We studied the virulence and tropism of...
The propensity of canine distemper virus (CDV) to spread the central nervous system is one primary features distemper. Therefore, we developed a reverse genetics based on neurovirulent Snyder Hill (SH) strain CDV (CDV(SH)) and show that this rapidly circumvents blood-brain blood-cerebrospinal fluid (CSF) barriers into subarachnoid space induce dramatic viral meningoencephalitis. use recombinant CDV(SH) (rCDV(SH)) expressing enhanced green fluorescent protein (EGFP) or red (dTomato)...