A5045542738- Antibiotic Resistance in Bacteria
- Enterobacteriaceae and Cronobacter Research
- Bacterial biofilms and quorum sensing
- Escherichia coli research studies
- Plant Pathogenic Bacteria Studies
- Nutrition, Genetics, and Disease
- Pneumocystis jirovecii pneumonia detection and treatment
- Bacterial Identification and Susceptibility Testing
- Pneumonia and Respiratory Infections
- Antimicrobial Resistance in Staphylococcus
- Bacteriophages and microbial interactions
- Pharmaceutical and Antibiotic Environmental Impacts
- Probiotics and Fermented Foods
Narodowy Instytut Leków
2021-2025
Background The war in Ukraine led to migration of Ukrainian people. Early 2022, several European national surveillance systems detected multidrug-resistant (MDR) bacteria related patients. Aim To investigate the genomic epidemiology New Delhi metallo-β-lactamase (NDM)-producing Providencia stuartii from patients among countries. Methods Whole-genome sequencing 66 isolates sampled 2022–2023 10 countries enabled whole-genome multilocus sequence typing (wgMLST), identification resistance genes,...
In February 2023, Escherichia coli sequence type (ST) 38 producing oxacillinase 244 (OXA-244-Ec ST38) was detected from three patients in a hospital western Poland. Overall, OXA-244-Ec ST38 colonised 13 wards between and June 2023. The outbreak investigated on site by an infection control team, the bacterial isolates were characterised microbiologically whole genome sequencing. We could not identify primary source of or reconstruct transmission sequence. some affected their groups linked...
Despite a scarcity of data, before 2022 Ukraine was already considered high-prevalence country for carbapenemase-producing Enterobacterales (CPE), and the situation has dramatically worsened during full-scale war with Russia. The aim this study to analyse CPEs isolated in Poland from victims Ukraine. included 65 CPE isolates March till February 2023, recovered 36 Polish medical centres 57 patients arriving Ukraine, differing largely by age reason hospitalisation. All were sequenced MiSeq ten...
Abstract Objectives To characterize carbapenemase-producing isolates of the Klebsiella pneumoniae hypervirulent (hvKp) clone ST23 in Poland. Methods Fifteen K. were identified by Polish surveillance Enterobacterales. These comprised a cluster with KPC-2 + NDM-1 (n = 7), 1) or enzymes from one hospital 2018, and sporadic 1), VIM-1 OXA-48 3), recovered 2009 to 2019 different towns. The sequenced Illumina MiSeq, followed MinION for six representatives. Clonality, phylogeny, serotypes,...
We sequenced all nonduplicate 934 VIM/IMP carbapenemase-producing Enterobacterales (CPE) reported in Poland during 2006-2019 and found ≈40% of the isolates (n = 375) were Enterobacter spp. During study period, incidence those bacteria gradually grew nearly entire country. The major factor affecting increase was clonal spread several E. hormaechei lineages responsible for multiregional interregional outbreaks (≈64% isolates), representing mainly pandemic sequence type (ST) 90 or...
Abstract Objectives To assess the spread of New Delhi metallo-β-lactamase-1 (NDM-1)-producing Klebsiella pneumoniae ST147 organisms in Poland since an introduction from Tunisia March 2015, including their phylogenetic position global population high-risk clone. Methods Out 8925 unique NDM-positive K. isolates identified April 2015 till December 2019, 126 isolates, Tunisian imports, were related by PFGE and blaNDM gene-carrying Tn125 transposon derivatives. Forty-seven representative...
Abstract Objectives To characterize VIM-type metallo-β-lactamase (MBL)-encoding genomic islands (GIs) in Pseudomonas aeruginosa and P. putida group isolates from Polish hospitals 2001–2015/16. Methods Twelve 20 producing VIM-like MBLs were selected a large collection of these based on epidemiological typing data. The organisms represented all major epidemic genotypes species spread Poland with chromosomally located blaVIM gene-carrying integrons. previously determined short-read sequences...
To elucidate the role of Klebsiella oxytoca species complex (KoSC) in epidemiology VIM-type MBL-producing Enterobacterales Poland.The study comprised all 106 VIM-positive KoSC isolates collected by Polish National Reference Centre for Susceptibility Testing during 2009-2019 from 60 institutions 35 towns. All were sequenced Illumina MiSeq, followed MinION sequencing selected organisms. Genomes subjected to bioinformatic analysis, addressing taxonomy, clonality, phylogeny and structural...
Abstract OXA-244-producing Escherichia coli (OXA-244-Ec) has disseminated in Europe, mostly the community. In Poland it spread since 2017, especially 2023, but contrast to other countries, all isolates have been identified hospitals so far. The ( n = 101) represented one large and two limited outbreaks different regions, multiple epidemiologically genetically non-related organisms. OXA-244-Ec population consisted of 14 STs, with ST38 dominating. belonged major lineages, Clusters A B,...
Pseudomonas putida group are described as low-incidence opportunistic pathogens, but also a significant reservoir of antimicrobial resistance (AMR) genes, including those metallo-β-lactamases (MBLs). Our objective was the molecular and genomic characterization MBL-producing P. (MPPP) isolates from Poland, focusing on population structures, successful genotypes MBL-encoding integrons.During country-wide MBL surveillance in spp., 59 non-duplicate MPPP were collected 36 hospitals 23 towns 2003...
Consecutive Polish regions have become endemic for NDM-1-producing Klebsiella pneumoniae ST11, followed by K. ST147. Since 2017 a significant increase in NDM-positive Enterobacter hormaechei cases has been observed.To investigate the origin and character of this E. hormaechei.The analysis included 160 NDM-producing cloacae complex isolates, recovered 2015-20 37 centres 9/16 regions. These were typed PFGE MLST, screened PCR-mapping NDM-1-encoding Tn125-like elements. Forty-four isolates...