A5070372821- Antibiotic Resistance in Bacteria
- Pharmaceutical and Antibiotic Environmental Impacts
- Antibiotics Pharmacokinetics and Efficacy
- Salmonella and Campylobacter epidemiology
- Vibrio bacteria research studies
- Bacterial Identification and Susceptibility Testing
- Pneumonia and Respiratory Infections
- Respiratory viral infections research
- Pleural and Pulmonary Diseases
- Antibiotic Use and Resistance
- Immune Cell Function and Interaction
- Urinary Tract Infections Management
- T-cell and B-cell Immunology
- Aquaculture disease management and microbiota
- Pneumocystis jirovecii pneumonia detection and treatment
- Chemical Reactions and Isotopes
- Innovation Policy and R&D
- Immunotherapy and Immune Responses
- Bacteriophages and microbial interactions
- Phenothiazines and Benzothiazines Synthesis and Activities
- Mast cells and histamine
- Biochemical and Structural Characterization
- Gut microbiota and health
- Escherichia coli research studies
- Antimicrobial Peptides and Activities
European Centre for Disease Prevention and Control
2019-2024
Finnish Institute for Health and Welfare
2019-2022
Uppsala University
2011-2020
Vilnius University
2010
To characterize the complete sequence, horizontal spread and stability of CTX-M-15-encoding multiresistance plasmid a Klebsiella pneumoniae strain involved in large nosocomial outbreak.The 220 kbp pUUH239.2 was completely sequenced using 454 technology. The conjugational host range, conjugation frequencies, fitness cost carriage were studied vitro. Conjugational during outbreak assessed retrospectively by multiplex PCR screening, restriction fragment length polymorphism PFGE.Plasmid encodes...
ABSTRACT Tigecycline is a glycylcycline antibiotic active against multidrug-resistant bacterial pathogens. The objectives of our study were to examine the potential Tet(A), Tet(K), Tet(M), and Tet(X) tetracycline resistance proteins acquire mutations causing tigecycline determine how this affects earlier classes tetracyclines. Mutations in all four tet genes caused significant increase MIC Escherichia coli , strains expressing mutant Tet(A) variants reached clinically relevant MICs (2...
Antibiotic resistance increasingly limits the success of antibiotic treatments, and physicians require new ways to achieve efficient treatment despite resistance. Resistance mechanisms against a specific class frequently confer increased susceptibility other classes, phenomenon designated collateral sensitivity (CS). An informed switch may thus enable resistant strains. CS occurs in many pathogens, but that generate hypersusceptibility are largely unknown. We identified several molecular...
Abstract Antibiotic resistance is a major challenge to global public health. Discovery of new antibiotics slow and ensure proper treatment bacterial infections strategies are needed. One way curb the development antibiotic design drug combinations where against one leads collateral sensitivity other drug. Here we study patterns globally distributed extended-spectrum β-lactamase CTX-M-15, find three non-synonymous mutations with increased mecillinam or piperacillin–tazobactam that...
Background The war in Ukraine led to migration of Ukrainian people. Early 2022, several European national surveillance systems detected multidrug-resistant (MDR) bacteria related patients. Aim To investigate the genomic epidemiology New Delhi metallo-β-lactamase (NDM)-producing Providencia stuartii from patients among countries. Methods Whole-genome sequencing 66 isolates sampled 2022–2023 10 countries enabled whole-genome multilocus sequence typing (wgMLST), identification resistance genes,...
To stepwise select tigecycline-resistant Escherichia coli mutants in vitro, determine the mutation rates, identify resistance mechanisms, level and cross-resistance to other antibiotic classes, evaluate fitness costs of tigecycline mechanisms investigate if same invitro-identified target genes were mutated clinical isolates. Spontaneous with reduced susceptibility selected on agar plates supplemented tigecycline. Resistance levels evaluated by performing MIC assays determining rates using...
The microbial ecosystem residing in the human gut is believed to play an important role horizontal exchange of virulence and antibiotic resistance genes that threatens health. While diversity gut-microorganisms their genetic content has been studied extensively, high-resolution insight into plasticity selective forces shaping individual genomes scarce. In a longitudinal study, we followed dynamics co-existing Escherichia coli lineages infant not receiving antibiotics. Using whole genome...
BackgroundVibriosis cases in Northern European countries and bordering the Baltic Sea increased during heatwaves 2014 2018.AimWe describe epidemiology of vibriosis genetic diversity Vibrio spp. isolates from Norway, Sweden, Denmark, Finland, Poland Estonia 2018, a year with an exceptionally warm summer.MethodsIn retrospective study, we analysed demographics, geographical distribution, seasonality, causative species severity non-travel-related 2018. Data sources included surveillance systems,...
Whole genome sequencing data of 874 Escherichia coli isolates carrying blaNDM-5 from 13 European Union/European Economic Area countries between 2012 and June 2022 showed the predominance sequence types ST167, ST405, ST410, ST361 ST648, an increasing frequency detection. Nearly a third (30.6%) these were associated with infections more than half (58.2%) predicted to be multidrug-resistant. Further spread E. would leave limited treatment options for serious infections.
Background Preliminary unpublished results of the survey carbapenem- and/or colistin-resistant Enterobacterales (CCRE survey) showed expansion carbapenemase-producing Klebsiella pneumoniae (CPKP) sequence type (ST) 39 in 12 15 participating Greek hospitals 2019. Aim We conducted a rapid to determine extent spread CPKP high-risk clones 2022 and compare distribution circulating these since 2013. Methods analysed whole genome sequences epidemiological data 310 K. isolates that were...
Analysis of 594 isolates Escherichia coli sequence type (ST)131 and its single locus variants carrying carbapenemase genes from 17 European Union/European Economic Area countries revealed acquisition 18 variants, mainly in ST131 clades A C. Most frequent were bla OXA-244 (n = 230) OXA-48 224), detected 14 12 countries, respectively. Isolates have increased rapidly since 2021. The increasing detection the E. high-risk lineage is a public health concern.
IgE antibodies, administered to mice together with their specific antigen, enhance antibody and CD4+ T cell responses this antigen. The effect is dependent on the low affinity receptor for IgE, CD23, must be expressed B cells. In vitro, IgE-antigen complexes are endocytosed via CD23 cells, which subsequently present antigen This mechanism has been suggested explain also IgE-mediated enhancement of immune in vivo. We recently found that CD23+ cells capture peripheral blood rapidly transport...
To determine the mechanism of resistance to antibiotic nitroxoline in Escherichia coli.Spontaneous nitroxoline-resistant mutants were selected at different concentrations nitroxoline. WGS and strain reconstruction used define genetic basis for resistance. The mechanistic was determined by quantitative PCR (qPCR) overexpression target genes. Fitness costs mutations cross-resistance other antibiotics also determined.Mutations transcriptional repressor emrR conferred low-level [nitroxoline MIC...
The objective of this study was to determine the fitness Escherichia coli mutants with reduced susceptibility tigecycline after exposure adverse conditions in vitro and vivo. Survival response low pH, bile salts, oxidative stress human serum examined for E. due single mutations that caused increased efflux (marR, lon) or impaired LPS (rfaC, rfaE, lpcA). An competition assay used growth defects. Competitive assessed using mouse infection models. MICs, exponential rates expression levels...
Background New Delhi metallo-beta-lactamase (NDM)-producing Providencia stuartii has been reported from European Union/European Economic Area (EU/EEA) countries with increasing frequency. During 2018 to 2022, 355 cases of NDM-producing P. were detected in seven hospitals reporting on NDM-production Enterobacterales Romania. Aim Our aim was determine the extent spread Methods We analysed whole genome sequences and epidemiological data 74 isolates collected six December 2021 September 2023....
We report an outbreak of invasive pneumococcal disease and pneumonia among shipyard workers, in Turku, Southwest Finland. In total, 31 confirmed six probable cases were identified between 3 May 28 November 2019. Streptococcus pneumoniae serotypes 12F, 4 8 isolated from blood cultures 25 cases. Occupational hygiene measures vaccination ca 4,000 workers are underway to control the at shipyard.
ABSTRACT Antimicrobial peptides (AMPs) are essential components of innate immunity in all living organisms, and these potent broad-spectrum antimicrobials have inspired several antibacterial development programs the past 2 decades. In this study, resistance to Gram-negative bacterium-specific peptide cycloviolacin O2 (cyO2), a member cyclotide family plant miniproteins, was characterized Salmonella enterica serovar Typhimurium LT2. Mutants isolated from serial passaging experiments...
In 2023, an increase of OXA-48-producing
Abstract Northern European countries and bordering the Baltic Sea have witnessed an increase of vibriosis cases during recent heatwaves. Here, we described epidemiology genetic diversity Vibrio isolates from Norway, Sweden, Denmark, Finland, Poland, Estonia, Latvia in 2018, a year with exceptionally warm summer. We conducted retrospective study analysed demographics, geographic distribution, seasonality, causative species, severity non-travel related 2018. Data sources included surveillance...