A5054552931- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- Antimicrobial Resistance in Staphylococcus
- Streptococcal Infections and Treatments
- Mycobacterium research and diagnosis
- Oral microbiology and periodontitis research
- Bacterial biofilms and quorum sensing
- Vibrio bacteria research studies
- Antibiotic Use and Resistance
- Antibiotics Pharmacokinetics and Efficacy
- Enterobacteriaceae and Cronobacter Research
- Escherichia coli research studies
- Antifungal resistance and susceptibility
- Infectious Encephalopathies and Encephalitis
- Thermal Regulation in Medicine
- Infections and bacterial resistance
- Genomics and Phylogenetic Studies
- Orthopedic Infections and Treatments
- Medical Device Sterilization and Disinfection
- Hematological disorders and diagnostics
- Microbial infections and disease research
- Microbial Metabolism and Applications
- Salmonella and Campylobacter epidemiology
- Dental Research and COVID-19
- Diphtheria, Corynebacterium, and Tetanus
Public Health Agency of Sweden
2023-2024
Karolinska Institutet
2012-2023
Karolinska University Hospital
2011-2021
University of Helsinki
2018
Helsinki University Hospital
2018
Morpho (United States)
2018
ABSTRACT Clinical isolates of Klebsiella pneumoniae producing NDM-1 carbapenemase from India ( n = 22), the United Kingdom 13), and Sweden 4) were subjected to multilocus sequence typing (MLST), automated repetitive sequence-based PCR (rep-PCR), serotyping, virulence gene screening, plasmid replicon typing. The most frequently detected MLST types (STs) ST14 13; all serotype K2), ST11, ST149, ST231, ST147. correlation between rep-PCR was excellent. IncA/C type 14). ST14, other successful...
Abstract Objectives When bloodstream infections are caused by resistant bacteria, rapid antimicrobial susceptibility testing (RAST) is important for adjustment of therapy. The EUCAST RAST method, directly from positive blood cultures, was validated in a multi-laboratory study Europe. Methods performed 40 laboratories northern Europe (NE) and 15 southern (SE) clinical cultures Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus or Streptococcus pneumoniae....
Background Preliminary unpublished results of the survey carbapenem- and/or colistin-resistant Enterobacterales (CCRE survey) showed expansion carbapenemase-producing Klebsiella pneumoniae (CPKP) sequence type (ST) 39 in 12 15 participating Greek hospitals 2019. Aim We conducted a rapid to determine extent spread CPKP high-risk clones 2022 and compare distribution circulating these since 2013. Methods analysed whole genome sequences epidemiological data 310 K. isolates that were...
Objectives: The need for rapid antibiotic susceptibility testing increases with escalating levels of antimicrobial resistance in Enterobacteriaceae. Our objective was to evaluate the accuracy reading EUCAST disc diffusion, ROSCO ESBL and carbapenemase detection kits Mast Carbapenemase Activity Test (CAT-ID) disc, after 6 h incubation. Methods: We used a collection 128 isolates Escherichia coli Klebsiella pneumoniae wide variety mechanisms. Inhibition zones read from digital photo images BD...
Invasive infections due to extended-spectrum-β-lactamase- and pAmpC-producing Escherichia coli (ESBL/pAmpC-EC) are an important cause of morbidity, often caused by the high-risk clone sequence type (ST131) isolates classified as extraintestinal pathogenic E. (ExPEC). The relative influence host immunocompetence versus microbiological virulence factors in acquisition outcome bloodstream (BSI) is poorly understood. Herein, we used whole-genome sequencing on 278 blood culture ESBL/pAmpC-EC from...
BackgroundThe number of cholera cases reported to the World Health Organization (WHO) in 2022 was more than double that 2021. Nine countries WHO European Region 51 vs five 2021.AimWe aimed confirm
Vancomycin-resistant Enterococcus faecium (VREfm) belonging to sequence type (ST)80 has become the predominant clonal lineage in Stockholm last three years. ST80 accounted for 75% and 46% of VRE cases 2018 2019, respectively, gave rise both vanA-type vanB-type outbreaks. Non-duplicate ST80-VREfm isolates (N = 188) were subjected whole genome sequencing. Genomic analysis revealed distinct transmission clusters. Our study indicated that difficulties detecting low-grade vancomycin-resistant by...
Methicillin-resistant Staphylococcus aureus (MRSA) USA300 isolates have been recognized globally, not only in community but also healthcare settings. were initially resistant to methicillin, resistance non-β-lactams has emerged with time. To evaluate the prevalence and antimicrobial susceptibility of Stockholm, we conducted a nine-year retrospective study. Of 5359 consecutive MRSA cases from 285 strains according pulsed-field gel electrophoresis pattern. these cases, repeated altered...
To examine performance of EUCAST disc diffusion and supplementary MIC methods for detection Enterobacteriaceae with reduced susceptibility to meropenem using screening recommendations. Sixty-one Nordic laboratories delivered data on (n = 61), semi-automated 23; VITEK2, n 20 Phoenix, 3) gradient 58) methods. The strains 27) included the major carbapenemase classes (A, 4; B, 9; D, 6) involved in global spread carbapenemase-producing (CPE) non-CPE 8) covering a range broth microdilution (BMD)...
Kytococcus schroeteri, a saprophyte of the human skin, may cause serious infections in immunocompromised host. Here, we describe case pneumonia and bacteremia due to schroeteri an patient, successfully treated with linezolid trimethoprim-sulfamethoxazole.
EUCAST rapid antimicrobial susceptibility testing (RAST) provides antibiotic results after 4 to 8 h of incubation. This study assessed the diagnostic performance and clinical usefulness RAST h. was a retrospective performed on blood cultures with Escherichia coli Klebsiella pneumoniae complex (K. variicola) at Karolinska University Laboratory (Stockholm, Sweden). The rate categorized categorical agreement (CA) standard 16-to-20-h disk diffusion (DD) method for piperacillin-tazobactam,...
The knowledge about the oral microbiota has increased greatly during past decade after introduction of high-throughput sequencing technologies. These culture-independent technologies have enabled detection as yet uncultured bacterial species that make up half 700 identified in microbiome. Similarly, mycobiome been shown to be much more diverse than previously expected. Currently, studies are underway clarify differences between microbiome health and disease with regard both involved...