Jean‐Philippe Rasigade
A5024203320- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Bacterial biofilms and quorum sensing
- Orthopedic Infections and Treatments
- Streptococcal Infections and Treatments
- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Neonatal and Maternal Infections
- Infective Endocarditis Diagnosis and Management
- Pneumonia and Respiratory Infections
- Antibiotic Resistance in Bacteria
- Clostridium difficile and Clostridium perfringens research
- Antibiotic Use and Resistance
- Antibiotics Pharmacokinetics and Efficacy
- Biochemical and Structural Characterization
- Evolution and Genetic Dynamics
- Urinary Tract Infections Management
- Infectious Diseases and Tuberculosis
- Hepatitis B Virus Studies
- Immune Response and Inflammation
- Microbial infections and disease research
- Diagnosis and treatment of tuberculosis
- Genomics and Phylogenetic Studies
- Viral Infections and Outbreaks Research
- Dermatology and Skin Diseases
Centre International de Recherche en Infectiologie
2015-2024
Université Claude Bernard Lyon 1
2015-2024
École Normale Supérieure de Lyon
2014-2024
Inserm
2014-2024
Centre National de la Recherche Scientifique
2015-2024
Hospices Civils de Lyon
2015-2024
Hôpital de la Croix-Rousse
2011-2023
Institut Mérieux (France)
2017-2022
École Pratique des Hautes Études
2017-2022
Université Paris Sciences et Lettres
2018-2022
Panton-Valentine leukocidin (PVL)-positive methicillin-susceptible Staphylococcus aureus and methicillin-resistant S. (MSSA MRSA, respectively) are both associated with severe infections, such as necrotizing pneumonia. The epidemiological profile of PVL-positive community-acquired (CA) MRSA has been extensively studied, but few corresponding data on MSSA available.The objectives the study were to investigate global population structure MSSA, compare it that reported for CA-MRSA, thus examine...
Background Coagulase-negative staphylococci, mainly Staphylococcus epidermidis, are the most frequent cause of late-onset sepsis (LOS) in neonatal intensive care unit (NICU) setting. However, recent reports indicate that methicillin-resistant, vancomycin-heteroresistant capitis could emerge as a significant pathogen NICU. We investigated prevalence, clonality and vancomycin susceptibility S. isolated from blood NICU infants compared these data to adult patients. Methodology/Principal...
Abstract Objectives When bloodstream infections are caused by resistant bacteria, rapid antimicrobial susceptibility testing (RAST) is important for adjustment of therapy. The EUCAST RAST method, directly from positive blood cultures, was validated in a multi-laboratory study Europe. Methods performed 40 laboratories northern Europe (NE) and 15 southern (SE) clinical cultures Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus or Streptococcus pneumoniae....
Bacterial factors favoring the unprecedented multidrug-resistant tuberculosis (MDR-TB) epidemic in former Soviet Union remain unclear. We utilized whole genome sequencing and Bayesian statistics to analyze evolutionary history, temporal emergence of resistance transmission networks MDR Mycobacterium complex isolates from Karakalpakstan, Uzbekistan (2001–2006). One clade (termed Central Asian outbreak, CAO) dating back 1974 (95% HPD 1969–1982) subsequently acquired mediating mutations eight...
ABSTRACT Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) was recognized in Europe and worldwide the late 1990s. Within a decade, several genetically geographically distinct CA-MRSA lineages carrying small SCC mec type IV V genetic elements Panton-Valentine leukocidin (PVL) emerged around world. In Europe, predominant strain belongs to clonal complex 80 (CC80) is resistant kanamycin/amikacin fusidic acid. CC80 first reported 1993 but relatively rare until It has since...
Abstract Transmission-driven multi-/extensively drug resistant (M/XDR) tuberculosis (TB) is the largest single contributor to human mortality due antimicrobial resistance. A few major clades of Mycobacterium complex belonging lineage 2, responsible for high prevalence MDR-TB in Eurasia, show outstanding transnational distributions. Here, we determined factors underlying emergence and epidemic spread W148 clade by genome sequencing Bayesian demogenetic analyses 720 isolates from 23 countries....
Epidemic community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) is associated with more severe and acute forms of osteomyelitis than healthcare-associated (HA-) MRSA. Although S. now recognized as a facultative intracellular pathogen, the contribution osteoblast invasion by CA-MRSA to pathogenesis unknown. Using an ex vivo model infection human osteoblasts, we demonstrated that strains diverse lineages share enhanced ability kill infected osteoblasts compared HA-MRSA....
Although Staphylococcus aureus persistence in osteoblasts, partly as small-colony variants (SCVs), can contribute to bone and joint infection (BJI) relapses, the intracellular activity of antimicrobials is not currently considered choice treatment strategies for BJI. Here, antistaphylococcal were evaluated their intraosteoblastic impact on emergence SCVs an ex vivo osteoblast model. Osteoblastic MG63 cells infected 2 h with HG001 S. aureus. After killing remaining extracellular bacteria...
Bone and joint infection, mainly caused by Staphylococcus aureus, is associated with significant morbidity mortality, characterized severe inflammation progressive bone destruction. Studies mostly focused on the interaction between S. aureus osteoblasts, matrix–forming cells, while interactions osteoclasts, only cells known to be able degrade bone, have been poorly explored. We developed an in vitro infection model of primary murine osteoclasts study direct impact live osteoclastogenesis...
Human intravenous immunoglobulin contains specific antitoxin antibodies that protect against necrotizing pneumonia caused by MRSA in a rabbit model.
Background Staphylococcus epidermidis orthopedic device infections are caused by direct inoculation of commensal flora during surgery and remain rare, although S. carriage is likely universal. We wondered whether infection strains might constitute a sub-population isolates with specific virulence ability. Biofilm formation invasion osteoblasts aureus contribute to bone joint recurrence protecting bacteria from the host-immune system most antibiotics. aimed determine could be distinguished...
Infective endocarditis (IE)(1) is a severe condition complicating 10–25% of Staphylococcus aureus bacteremia. Although host-related IE risk factors have been identified, the involvement bacterial features in complication still unclear. We characterized strictly defined and bacteremia isolates searched for discriminant features. S. causing community-acquired, definite native-valve (n = 72) 54) were collected prospectively as part French multicenter cohort. Phenotypic traits previously...
Staphylococcus aureus bone and joint infection (BJI) is associated with significant rates of chronicity relapse. In this study, we investigated how S. able to adapt the human environment by comparing isolates from single patients persisting or relapsing BJIs that were recovered during initial recurrent BJI episodes. vitro in vivo assays whole-genome sequencing analyses revealed induced a reduced inflammatory response, formed more biofilms, persisted longer intracellular compartments host...
Multidrug-resistant (MDR) Mycobacterium tuberculosis complex (MTBC) strains are a serious health problem in India, also contributing to one-fourth of the global MDR (TB) burden. About 36% MTBC reported fluoroquinolone (FQ) resistant leading high pre-extensively drug-resistant (pre-XDR) and XDR-TB (further resistance against bedaquiline and/or linezolid) rates. Still, factors driving MDR/pre-XDR epidemic India not well defined. In retrospective study, we analyzed 1852 consecutive obtained...
We investigated the population structure of Staphylococcus aureus clonal complex CC121 by mutation discovery at 115 genetic housekeeping loci from each 154 isolates, sampled on five continents between 1953 and 2009. In addition, we pyro-sequenced genomes ten representative isolates. The genome-wide SNPs that were ascertained revealed evolutionary history CC121, indicating least six major clades (A to F) within dating its most recent common ancestor pre-antibiotic era. toxin gene complement...
Staphylococcus aureus causes severe forms of community-acquired pneumonia (CAP), namely staphylococcal pleuropneumonia in young children and necrotising older patients. Methicillin resistance the Panton-Valentine leukocidin (PVL) toxin, as well less specific factors, have been associated with poor outcome CAP, but their roles are unclear.A prospective multicentre cohort study CAP was conducted 77 paediatric adult intensive care units France between January 2011 December 2016. After...
Abstract Background Staphylococcus aureus is a well-armed pathogen prevalent in severe infections such as endocarditis and osteomyelitis. Fibronectin-binding proteins A B, encoded by fnb A/B, are major pathogenesis determinants these through their involvement S. adhesion to invasion of host cells. Sub-minimum inhibitory concentrations (sub-MICs) antibiotics, frequently occurring vivo because impaired drug diffusion at the infection site, can alter phenotype. We therefore investigated impact...