Helena Žemličková
A5050797630- Antibiotic Resistance in Bacteria
- Pneumonia and Respiratory Infections
- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Bacterial Infections and Vaccines
- Antibiotic Use and Resistance
- Bacterial biofilms and quorum sensing
- Enterobacteriaceae and Cronobacter Research
- Streptococcal Infections and Treatments
- Antibiotics Pharmacokinetics and Efficacy
- Pharmaceutical and Antibiotic Environmental Impacts
- Salmonella and Campylobacter epidemiology
- Infections and bacterial resistance
- Respiratory viral infections research
- Vibrio bacteria research studies
- Viral gastroenteritis research and epidemiology
- Preterm Birth and Chorioamnionitis
- Microbial infections and disease research
- Urinary Tract Infections Management
- Genomics and Phylogenetic Studies
- Yersinia bacterium, plague, ectoparasites research
- Clostridium difficile and Clostridium perfringens research
- Plant Pathogenic Bacteria Studies
- Pneumocystis jirovecii pneumonia detection and treatment
- Actinomycetales infections and treatment
National Institute of Public Health
2016-2025
Charles University
2016-2025
University Hospital Kralovske Vinohrady
2021-2024
University Hospital Hradec Králové
2017-2022
University of Hradec Králové
2018
Hospital for Tropical Diseases
2016
Centre Hospitalier Régional et Universitaire de Nancy
2016
National Institute of Public Health
2013
National Institutes of Health
2012
Janssen (Germany)
2011
Infections due to antibiotic-resistant bacteria are threatening modern health care. However, estimating their incidence, complications, and attributable mortality is challenging. We aimed estimate the burden of infections caused by public concern in countries EU European Economic Area (EEA) 2015, measured number cases, deaths, disability-adjusted life-years (DALYs).We estimated incidence with 16 antibiotic resistance-bacterium combinations from Antimicrobial Resistance Surveillance Network...
The widespread use of antibiotics in association with high-density clinical care has driven the emergence drug-resistant bacteria that are adapted to thrive hospitalized patients. Of particular concern globally disseminated methicillin-resistant Staphylococcus aureus (MRSA) clones cause outbreaks and epidemics associated health care. most rapidly spreading tenacious health-care-associated clone Europe currently is EMRSA-15, which was first detected UK early 1990s subsequently spread...
ABSTRACT The implementation of routine whole-genome sequencing (WGS) promises to transform our ability monitor the emergence and spread bacterial pathogens. Here we combined WGS data from 308 invasive Staphylococcus aureus isolates corresponding a pan-European population snapshot, with epidemiological resistance data. Geospatial visualization is made possible by generic software tool designed for public health purposes that available at project URL (...
To estimate the proportion of methicillin-resistant Staphylococcus aureus (MRSA) isolates from humans that were sequence type (ST) 398, we surveyed 24 laboratories in 17 countries Europe 2007. Livestock-associated MRSA ST398 accounted for only a small humans; most Netherlands, Belgium, Denmark, and Austria.
Due to the lack of fossil evidence, timescales bacterial evolution are largely unknown. The speed with which genetic change accumulates in populations pathogenic bacteria, however, is a key parameter that crucial for understanding emergence traits such as increased virulence or antibiotic resistance, together forces driving pathogen spread. Methicillin-resistant Staphylococcus aureus (MRSA) common cause hospital-acquired infections. We have investigated an MRSA strain (ST225) highly...
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is a potentially useful tool for the detection antimicrobial resistance, especially that conferred by β-lactamases. Here we describe modification previously reported MALDI-TOF MS meropenem hydrolysis assay. The modified method was validated on 108 carbapenemase-producing members Enterobacteriaceae, two NDM-1-producing Acinetobacter baumannii isolates, and 35 carbapenem-resistant enterobacteria...
Significance In many clinically important bacteria, antibiotic resistance genes are primarily carried on plasmids. These can spread horizontally between different strains and species. However, current surveillance systems track chromosomal lineages of bacteria only, leading to an incomplete understanding how spreads, from within individual hospital across country borders. We present integrated, high-resolution analysis both chromosome plasmid sequences using Klebsiella pneumoniae isolates...
BackgroundGenomic surveillance using quality-assured whole-genome sequencing (WGS) together with epidemiological and antimicrobial resistance (AMR) data is essential to characterise the circulating Neisseria gonorrhoeae lineages their association patient groups (defined by demographic factors). In 2013, European gonococcal population was characterised genomically for first time. We describe in 2018 identify emerging or vanishing associated AMR characteristics of patients, elucidate recent...
BackgroundRegular quality-assured whole-genome sequencing linked to antimicrobial resistance (AMR) and patient metadata is imperative elucidate the shifting gonorrhoea epidemiology, both nationally internationally. We aimed examine gonococcal population in European Economic Area (EEA) 2020, emerging disappearing lineages associated with AMR metadata, compare 2013 2018 data, explain changes epidemiology.MethodsIn this retrospective genomic surveillance study, we analysed consecutive isolates...
Streptococcus pneumoniae of serotype 3 possess a mucoid capsule and cause disease associated with high mortality rates relative to other pneumococci. Phylogenetic analysis complete reference genome 81 draft sequences from clonal complex 180, the predominant clone in much world, found most sampled isolates belonged clade affected by few diversifying recombinations. However, indicate significant genetic variation has accumulated over complex's entire history. Two closely related genomes, one...
The aim of this study was to characterize the first cases and outbreaks OXA-48-like-producing Enterobacteriaceae recovered from hospital settings in Czech Republic. From 2013 2015, 22 Klebsiella pneumoniae isolates, 3 Escherichia coli 1 Enterobacter cloacae isolate producing OXA-48-like carbapenemases were isolated 20 patients. Four patients colonized or infected by two three different producers. K. isolates classified into nine sequence types (STs), with ST101 being predominant (n = 8). E....
The aim of the present study was to characterize sporadic cases and an outbreak NDM-like-producing Enterobacteriaceae recovered from hospital settings, in Czechia. During 2016, 18 Entrobacteriaceae isolates including 10 Enterobacter cloacae complex (9 E. xiangfangensis 1 asburiae), 4 Escherichia coli, Kluyvera intermedia, Klebsiella pneumoniae, oxytoca, Raoultella ornithinolytica that produced NDM-like carbapenemases were isolated 15 patients. Three patients colonized or infected by two...
BackgroundInvasive infections caused by Staphylococcus aureus have high clinical and epidemiological relevance. It is therefore important to monitor the S. trends using suitable methods.AimThe study aimed describe of bloodstream (BSI) meticillin-resistant (MRSA) meticillin-susceptible (MSSA) in European Union (EU) Economic Area (EEA).MethodsAnnual data on BSI from 2005 2018 were obtained Antimicrobial Resistance Surveillance Network (EARS-Net). Trends assessed at EU/EEA level adjusting for...
The European Gonococcal Antimicrobial Surveillance Programme (Euro-GASP) performs annual sentinel surveillance of Neisseria gonorrhoeae susceptibility to therapeutically relevant antimicrobials across the Union/European Economic Area (EU/EEA). We present Euro-GASP results from 2019 (26 countries), linked patient epidemiological data, and compared with data previous years.Agar dilution minimum inhibitory concentration (MIC) gradient strip methodologies were used determine antimicrobial (using...
ABSTRACT The objective of this study was to perform molecular surveillance for assessing the spread carbapenemase-producing Pseudomonas aeruginosa in Czech hospitals. One hundred thirty-six isolates were recovered from 22 hospitals located throughout country. Sequence type 357 (ST357) dominated ( n = 120) among carbapenemase producers. seventeen produced IMP-type (IMP-7 [ 116] and IMP-1 1]) metallo-β-lactamases (MβLs), 15 VIM-2 MβL, remaining expressed GES-5 enzyme. bla IMP -like genes three...
Abstract Lytic bacteriophages are valuable therapeutic agents against bacterial infections. There is continual effort to obtain new phages increase the effectivity of phage preparations emerging phage-resistant strains. Here we described genomic diversity spontaneous host-range mutants kayvirus 812. Five mutant were isolated as rare plaques on Staphylococcus aureus The host range 812-derived was 42% higher than wild type, determined a set 186 methicillin-resistant S. strains representing...
Building the European Antimicrobial Resistance Surveillance network in Veterinary medicine (EARS-Vet) was proposed to strengthen One Health antimicrobial resistance (AMR) surveillance approach.
Aminopenicillins are recommended agents for non-invasive Haemophilus influenzae infections. One of the mechanisms resistance to β-lactams is alteration transpeptidase region penicillin binding protein 3 (PBP3) which caused by mutations in ftsI gene. It was shown that exposure beta-lactams has a stimulating effect on increase prevalence H. strains with non-enzymatic mechanism resistance. The aim our study compare mutational potential ampicillin and cefuroxime strains, determination minimum...
Abstract Background Data from surveillance on antibiotic resistance have shown an increasing prevalence of non-enzymatic (β-lactamase-negative ampicillin-resistant) to β-lactam antibiotics among H. influenzae strains in the Czech Republic. Aminopenicillins are recommended agents for non-invasive Haemophilus infections. The phenomenon β-lactams is complicated by fact that phenotypic detection PBP3 with specific amino acid substitutions (rPBP3) challenging, since rPBP3 isolates repeatedly been...