A5080583446- Streptococcal Infections and Treatments
- Neonatal and Maternal Infections
- Antimicrobial Resistance in Staphylococcus
- Pneumonia and Respiratory Infections
- Bacterial Identification and Susceptibility Testing
- Infective Endocarditis Diagnosis and Management
- Genomics and Phylogenetic Studies
- Bacterial biofilms and quorum sensing
- RNA and protein synthesis mechanisms
- Sepsis Diagnosis and Treatment
- Obsessive-Compulsive Spectrum Disorders
- Otolaryngology and Infectious Diseases
- Bacterial Genetics and Biotechnology
- Maternal and Neonatal Healthcare
- Antibiotic Resistance in Bacteria
- Bacterial Infections and Vaccines
- Neonatal Health and Biochemistry
- Microbial Community Ecology and Physiology
- Antibiotic Use and Resistance
- RNA modifications and cancer
- Oral microbiology and periodontitis research
- Probiotics and Fermented Foods
- Botulinum Toxin and Related Neurological Disorders
- Urticaria and Related Conditions
- Respiratory and Cough-Related Research
Istituto Superiore di Sanità
2016-2025
Tel Aviv University
2021-2022
University College London
2021-2022
University of Calgary
2021-2022
Schneider Children's Medical Center
2021
National Institute of Health
2007-2020
Mylan (South Africa)
2020
Institute of Studies for the Integration of Systems
2020
Ministero della Salute
2005-2016
Policlinico di Modena
2016
ABSTRACT The past 2 decades have brought worrying increases in severe Streptococcus pyogenes diseases globally. To investigate and compare the epidemiological patterns of these within Europe, data were collected through a European Union FP-5-funded program (Strep-EURO). Prospective population-based surveillance S. infection diagnosed during 2003 2004 was undertaken 11 countries across Europe (Cyprus, Czech Republic, Denmark, Finland, France, Germany, Greece, Italy, Romania, Sweden, United...
ABSTRACT In an attempt to compare the epidemiology of severe Streptococcus pyogenes infection within Europe, prospective data were collected through Strep-EURO program. Surveillance for cases S. diagnosed during 2003 and 2004 was undertaken in 11 countries across Europe by using a standardized case definition questionnaire. Patient as well bacterial isolates characterized T M/ emm typing, selected strains analyzed presence superantigen genes. Data clinical microbiological patterns infections...
Abstract Streptococcus agalactiae (Group B , GBS) is a commensal of the digestive and genitourinary tracts humans that emerged as leading cause bacterial neonatal infections in Europe North America during 1960s. Due to lack epidemiological genomic data, reasons for this emergence are unknown. Here we show by comparative genome analysis phylogenetic reconstruction 229 isolates rise human GBS corresponds selection worldwide dissemination only few clones. The parallel expansion clones preceded...
A collection of Enterococcus faecalis strains from clinical isolates, healthy individuals and the environment was screened for presence virulence factor genes, such as those collagen-binding protein (ace), endocarditis antigen (efaA), haemolysin activator (cylA), gelatinase (gelE), aggregation substances (asa1 asa373), a surface (esp) two novel putative antigens (EF0591 EF3314). Apart some genes that were present in all (ace, efaA EF3314), gelE gene most common factor, although its did not...
We recently described the presence of 3 pilus variants in human pathogen group B streptococcus (GBS; also known as Streptococcus agalactiae), each encoded by a distinct pathogenicity island, well ability components to elicit protection mice against homologous challenge. To determine whether vaccine containing combination proteins from types could provide broad protection, we analyzed pili distribution and conservation 289 clinical isolates. found that sequences island are conserved, all...
There is insufficient population-based data on group B streptococcus (GBS) late-onset disease (LOD). Risk factors and routes of GBS transmission are poorly understood.A prospective, cohort study was conducted to collect incidence LOD evaluate infections over an 8-year period (2003-2010). Starting from January 2007, maternal rectovaginal breast milk cultures were routinely collected confirmation the diagnosis assess culture status.The rate 0.32 per 1000 live births (1.4 0.24 for preterm term...
Current literature shows that secondary bacterial infections, although less frequent than in previous influenza pandemics, affect COVID-19 patients. Mycoplasma pneumoniae, Staphylococcus aureus, Legionella pneumophila, Streptococcus Haemophilus and Klebsiella spp. are the main species isolated. Of note, Mycobacterium tuberculosis-COVID-19 coinfections also reported. However, coinfection rates increase patients admitted intensive care units, those diseases can be due to super-infections by...
Background: Streptococcus pyogenes or group A streptococcus (GAS) is an important human pathogen responsible for a broad range of infections, from uncomplicated to more severe and invasive diseases with high mortality morbidity. Epidemiological surveillance has been crucial detect changes in the geographical temporal variation disease pattern; this purpose M protein gene (emm) typing most widely used genotyping method, than 200 emm types recognized. Molecular epidemiological data have also...
One-hundred and twenty-eight enterococcal isolates were examined for their ability to form biofilm in relation the presence of gene encoding surface protein (esp), production gelatinase source isolation. Neither esp nor seemed be required formation: both Enterococcus faecalis faecium did not show a correlation between either or formation. However, E. while was found from source, together only strains clinical settings, suggesting that there exists synergy these factors which serves as an...
ABSTRACT Group B streptococci (GBS) comprising three different sets of isolates (31 invasive, 36 noninvasive, and 24 colonizing isolates) were collected in Italy during the years 2002 to 2005. Clonal groups established by pulsed-field gel electrophoresis (PFGE), selected studied multilocus sequence typing (MLST). GBS also characterized classical molecular techniques for serotyping protein gene antibiotic resistance profiling. Some serotypes significantly associated with a particular isolate...
ABSTRACT Streptococcus pyogenes infections often fail to respond antibiotic therapy, leading persistent throat carriage and recurrent infections. Such failures cannot always be explained by the occurrence of resistance determinants, it has been suggested that S. may enter epithelial cells escape treatment. We investigated 289 strains isolated from different clinical sources evaluate their ability form biofilm as an alternative method treatment host defenses. Up 90% isolates, both invasive...
ABSTRACT We developed a group B streptococcus multiplex PCR assay which allows, by direct analysis of the amplicon size, determination surface protein antigen genes alpha-C protein, epsilon Rib, Alp2, Alp3, and Alp4. The offers rapid simple method subtyping Streptococcus agalactiae based on genes.
A biofilm-negative transposon mutant was created from an Enterococcus faecalis strain that produces a lot of biofilm. The had been inserted in the second gene locus consisting 4 open-reading frames, designated bop (biofilm on plastic surfaces). nonpolar deletion this and parts 2 flanking genes created; production biofilm by significantly enhanced, compared with wild-type strain. Expression downstream lower than biofilm-enhanced mutant. Transformation into partially restored Mice challenged...
Background. Group B Streptococcus (GBS) is a major cause of neonatal sepsis and meningitis. A vaccine targeting pregnant women could protect infants through placentally transferred antibodies. The association between GBS maternal antibody concentrations the risk infection has been investigated in US African populations. Here we studied naturally acquired immunoglobulin G (IgG) responses to capsular polysaccharides (CPS) pilus proteins European women. Methods. Maternal sera were prospectively...
Forensic microbiology is a relatively new discipline, born in part thanks to the development of advanced methodologies for detection, identification and characterization microorganisms, also relation growing impact infectious diseases iatrogenic origin. Indeed, increased application medical practices, such as transplants, which require immunosuppressive treatments, demand prosthetic installations, associated with an increasing threat antimicrobial resistance, have led rise number infections...
Members of a family repeat-containing surface proteins group B streptococci (GBS) defined by the alpha C and Rib exhibit size variability cross-reactivity have been studied as potential vaccine components. We report evidence horizontal DNA transfer with subsequent recombination mechanism generating diversity within this antigen family. Alp2 Alp3 are additional members protein identified in strains emerging GBS serotypes V VIII. Each contains an overall genetic organization highly similar to...
ABSTRACT Occurrence of the hsp70 ( dnaK ) gene was investigated in various members domain Archaea comprising both euryarchaeotes and crenarchaeotes hyperthermophilic bacteria Aquifex pyrophilus Thermotoga maritima representing deepest offshoots phylogenetic trees bacterial 16S rRNA sequences. The not detected 8 10 archaea examined but found A. T. , from which it cloned sequenced. Comparative analyses HSP70 amino acid sequences encoded these genes, others databases, showed that (i) accordance...
Aim To evaluate the relationship between diagnosis and clinical course of Tourette syndrome group A Streptococcus (GAS). Method GAS infections, anti-streptococcal, anti-basal ganglia antibodies (ABGA) were compared 168 patients (136 males, 32 females) with syndrome; (median [range] age [25th–75th centile] 10y [8–11y]); median duration (25th–75th centile), 3y (1y 3mo–5y 9mo) a comparison 177 (117 60 epileptic or sleep disorders centile], [8y–1y 6mo]). One hundred forty-four followed up at...
ABSTRACT We report the results from first international multicenter external quality assessment (EQA) studies for molecular and serological typing of group B streptococcus (GBS) strains as part DEVANI ( De sign a V accine gainst N eonatal I nfections), pan-European program. A questionnaire-based surveillance was undertaken among eight laboratories participating in six not 13 countries order to assess their current microbiological procedures GBS screening, diagnosis, typing. three EQA...
Group B Streptococcus (GBS) is a major cause of invasive disease in neonates the United States. Surveillance GBS Minnesota, USA, during 2000-2010 yielded 449 isolates from infants; 257 had early-onset (EO) (by age 6 days) and 192 late-onset (LO) (180 at 7-89 days, 12 90-180 days). Isolates were characterized by capsular polysaccharide serotype surface-protein profile; types III Ia predominated. However, because previously uncommon IV constitutes 5/31 EO 2010, twelve type collected studied...
Building the European Antimicrobial Resistance Surveillance network in Veterinary medicine (EARS-Vet) was proposed to strengthen One Health antimicrobial resistance (AMR) surveillance approach.