A5011238769- Antibiotic Resistance in Bacteria
- Pharmaceutical and Antibiotic Environmental Impacts
- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Vibrio bacteria research studies
- Pneumonia and Respiratory Infections
- Escherichia coli research studies
- Antibiotic Use and Resistance
- Bacteriophages and microbial interactions
- Bacterial Infections and Vaccines
- Salmonella and Campylobacter epidemiology
- Infective Endocarditis Diagnosis and Management
- Streptococcal Infections and Treatments
- Bacterial Genetics and Biotechnology
- Antibiotics Pharmacokinetics and Efficacy
- Bacterial biofilms and quorum sensing
- Enterobacteriaceae and Cronobacter Research
- Clostridium difficile and Clostridium perfringens research
- Nosocomial Infections in ICU
- Mycobacterium research and diagnosis
- Urinary Tract Infections Management
- Pharmaceutical Economics and Policy
- Gut microbiota and health
- Rabies epidemiology and control
- Infections and bacterial resistance
Université de Limoges
2016-2025
Inserm
2015-2024
CNR de la Résistance aux Antibiotiques
2013-2024
Anti-infectieux : supports moléculaires des résistances et innovations thérapeutiques
2013-2024
Hôpital Universitaire Dupuytren
2011-2024
Centre Hospitalier Universitaire de Limoges
2014-2023
Institut National de Recherche en Santé Publique
2018
Institut National de Santé Publique
2015
Centre National de la Recherche Scientifique
1994-2015
Technical University of Denmark
2015
The spread of antibiotic-resistant bacteria is a growing problem and public health issue. In recent decades, various genetic mechanisms involved in the resistance genes among have been identified. Integrons - elements that acquire, exchange, express embedded within gene cassettes (GC) are one these mechanisms. widely distributed, especially Gram-negative bacteria; they carried by mobile elements, plasmids, transposons, which promote their bacterial communities. Initially studied mainly...
ObjectivesIntegrons are bacterial genetic elements that can capture and express genes contained in mobile cassettes. Integrons have been described worldwide Gram-negative bacteria a marker of antibiotic resistance. We developed specific sensitive Taqman® probe-based real-time PCR method with three different primer–probe pairs for simultaneous detection the main classes integron.
Class 1 integrons are widespread genetic elements that allow bacteria to capture and express gene cassettes usually promoterless. These play a major role in the dissemination of antibiotic resistance among Gram-negative bacteria. They typically consist (intI) encoding an integrase (that catalyzes cassette movement by site-specific recombination), recombination site (attI1), promoter (Pc) responsible for expression inserted cassettes. The Pc can occasionally be combined with second designated...
The type III secretion system is an important Pseudomonas aeruginosa-virulence determinant in animal models of infection and humans. Antibody-mediated inhibition the PcrV protein, essential component this system, might abrogate aeruginosa ability to damage epithelial cells, neutrophils, macrophages, thereby limiting its pathogenicity. objective trial was determine safety, pharmacokinetics, prevent ventilator-associated pneumonia KB001, a recombinant, PEGylated, engineered, human Fab'...
Class 1 integrons are genetic systems that enable bacteria to capture and express gene cassettes. These integrons, when isolated in clinical contexts, most often carry antibiotic resistance They play a major role the dissemination of among Gram-negative bacteria. The key element is integrase, which allows cassettes be acquired shuffled. Planktonic culture experiments have shown integrase expression regulated by bacterial SOS response. In natural settings, however, generally live biofilms,...
Wastewaters (WW) are important sources for the dissemination of antimicrobial resistance (AMR) into environment. Hospital WW (HWW) contain higher loads micro-pollutants and AMR markers than urban (UWW). Little is known about long-term dynamics H U impact their joined treatment on general burden AMR. Here, we characterized resistome, microbiota eco-exposome signature 126 samples treated separately three years, then mixed, over one year. Multi-variate analysis machine learning revealed a...
ABSTRACT Twenty Acinetobacter baumannii strains resistant to various antibiotics were analyzed for integron content and sequences of the amplification products. Sixteen clinical isolates had a class 1 integron, 2 contained an additional or but no 3 was detected. Thirteen integrons with single cassette: aac ( )- Ia (9 strains), ant ")- (2 6 ′)- Ib strains); oxa20 cassettes unknown gene; containing oxa3 cassette truncated by IS 6100 . The remaining harbored gene previously found in...
Integrons are bacterial genetic elements able to capture and express genes contained within mobile gene cassettes. Gene cassettes expressed via a Pc promoter can be excised from or integrated into the integron by integrase IntI. Although mechanisms of cassette integration excision well known, kinetics modes shuffling leading new arrays remain puzzling. It has been proposed that under antibiotic selective pressure, IntI-mediated rearrangements generate variants in which weakly moves closer...
ABSTRACT The objective of this study was to perform an inventory the extended-spectrum-β-lactamase (ESBL)-producing Enterobacteriaceae isolates responsible for infections in French hospitals and assess mechanisms associated with ESBL diffusion. A total 200 nonredundant ESBL-producing strains isolated from clinical samples were collected during a multicenter performed 18 representative hospitals. Antibiotic resistance genes identified by PCR sequencing experiments. clonal relatedness between...
Building the European Antimicrobial Resistance Surveillance network in Veterinary medicine (EARS-Vet) was proposed to strengthen One Health antimicrobial resistance (AMR) surveillance approach.
Prokaryotic insertion sequences (IS) are pivotal in the propagation of bacterial multidrug resistance, with IS 91 notably linked to virulence and antibiotic resistance genes. However, precise mechanism by which contributes gene dissemination remains elusive. Unique among its family, features a small open reading frame (orf) upstream tnpA transposase gene, potentially encoding 121-amino acid protein, orf121 , may be translationally coupled . Using genetic system based on mating-out assay...
ABSTRACT Enterobacter aerogenes BM2688, which is resistant to multiple antibiotics, and its aminoglycoside-susceptible derivative BM2688-1 were isolated from the same clinical sample. Strain BM2688 harbored plasmid pIP833, carries a class 1 integron, In40, containing (in addition qacEΔ1 sul1 , are characteristic of integrons) four gene cassettes: aac(6′)-Ib qacF cmlA2 oxa-9 . The had 83.7% identity with previously described nonenzymatic chloramphenicol resistance cmlA1 gene. conferred...
Since its initial identification in epidemic multidrug-resistant Salmonella enterica serovar Typhimurium DT104 strains, several SGI1 variants, lineages, and SGI1-related elements (SGI2, PGI1, AGI1) have been described many bacterial genera ( , Proteus Morganella Vibrio Shewanella etc.). They constitute a family of multidrug resistance site-specific integrative acquired by horizontal gene transfer, being the best-characterized element. The transfer SGI1/PGI1 into other is public health...