A5009061160- Pharmaceutical and Antibiotic Environmental Impacts
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- SARS-CoV-2 detection and testing
- Evolution and Genetic Dynamics
- Biosensors and Analytical Detection
- Gut microbiota and health
- COVID-19 diagnosis using AI
- Microbial infections and disease research
- Salmonella and Campylobacter epidemiology
- Wastewater Treatment and Reuse
- Healthcare and Environmental Waste Management
- Fecal contamination and water quality
- SARS-CoV-2 and COVID-19 Research
- Pneumocystis jirovecii pneumonia detection and treatment
- Bacterial biofilms and quorum sensing
- Genomics and Phylogenetic Studies
- Water Treatment and Disinfection
- Parasitic Infections and Diagnostics
- Environmental Toxicology and Ecotoxicology
- Water Governance and Infrastructure
- Aquaculture disease management and microbiota
- Escherichia coli research studies
- Bauxite Residue and Utilization
- DNA and Nucleic Acid Chemistry
Inserm
2012-2025
University of Idaho
2016-2025
Université de Limoges
2012-2025
École Nationale Vétérinaire de Toulouse
2015
Université Claude Bernard Lyon 1
2015
Centro Nacional de Biotecnología
2015
Consejo Superior de Investigaciones Científicas
2015
Newcastle University
2015
Technical University of Denmark
2015
University of Minnesota
2015
The spread of antibiotic-resistant bacteria is a growing problem and public health issue. In recent decades, various genetic mechanisms involved in the resistance genes among have been identified. Integrons - elements that acquire, exchange, express embedded within gene cassettes (GC) are one these mechanisms. widely distributed, especially Gram-negative bacteria; they carried by mobile elements, plasmids, transposons, which promote their bacterial communities. Initially studied mainly...
Abstract The rapid spread of antibiotic resistance among bacterial pathogens is a serious human health threat. While range environments have been identified as reservoirs genes (ARGs), we lack understanding the origins these ARGs and their from environment to clinic. This partly due our inability identify natural hosts mobile genetic elements that mediate this spread, such plasmids integrons. Here demonstrate in vivo proximity-ligation method Hi-C can reconstruct known plasmid-host...
Wastewater surveillance for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an emerging approach to help identify risk of a disease (COVID-19) outbreak. This tool can contribute public health at both community (wastewater treatment system) and institutional (e.g., colleges, prisons, nursing homes) scales. paper explores successes, challenges, lessons learned from initial wastewater efforts colleges university systems inform future research, development implementation. We...
As mobile genetic elements, plasmids are central for our understanding of antimicrobial resistance spread in microbial communities. Plasmids can have varying fitness effects on their host bacteria, which will markedly impact role as vectors. Using a plasmid population model, we first show that beneficial interact with higher number hosts than costly when embedded community multiple and plasmids. We then analyse the network natural host-plasmid wastewater from Hi-C metagenomics dataset....
Biofilms dominate microbial life, and their importance for human health the environment can no longer be dismissed. Nevertheless many of processes governing this form growth are still poorly understood. This includes horizontal exchange genetic information, which is a major driver in bacterial evolution rapid adaptation, exemplified by alarming spread multi-drug resistance among pathogens mediated plasmids. often considered hot spot gene transfer, yet several studies have shown that plasmid...
Produce is one of the most popular food commodities. Unfortunately, leafy greens can be a reservoir transferable antibiotic resistance genes. We found that IncF and IncI plasmids were prevalent plasmid types in E. coli isolates from produce. This study highlights importance rare microbiome associated with produce as source genes might escape cultivation-independent detection, yet may transferred to human pathogens or commensals.
Abstract Plasmids are mobile genetic elements found in many clades of Archaea and Bacteria. They drive horizontal gene transfer, impacting ecological evolutionary processes within microbial communities, hold substantial importance human health biotechnology. To support plasmid research provide scientists with data an unprecedented diversity sequences, we introduce the IMG/PR database, a new resource encompassing 699 973 sequences derived from genomes, metagenomes metatranscriptomes. is first...
Multidrug resistant bacterial pathogens have become a serious global human health threat, and conjugative plasmids are important drivers of the rapid spread resistance to last-resort antibiotics. Whereas antibiotics been shown select for adaptation their new hosts, or vice versa, general evolutionary mechanism has not yet emerged. Here we conducted an experimental evolution study aimed at determining patterns plasmid-bacteria evolution. Specifically, found that large plasmid follows same...
Abstract Bacterial plasmids substantially contribute to the rapid spread of antibiotic resistance, which is a crisis in healthcare today. Coevolution and their hosts promotes this resistance by ameliorating cost plasmid carriage. However, our knowledge plasmid–bacteria coevolution solely based on studies done well-mixed liquid cultures, even though biofilms represent main way bacterial life Earth are responsible for most infections. The spatial structure heterogeneity provided known lead...
Self-transmissible multidrug resistance (MDR) plasmids are a major health concern because they can spread antibiotic to pathogens. Even though most pathogens form biofilms, little is known about how MDR persist and evolve in biofilms. We hypothesize that (i) biofilms act as refugia of by retaining them the absence antibiotics longer than well-mixed planktonic populations (ii) evolutionary trajectories account for improvement plasmid persistence over time differ between populations. In this...
Abstract Despite the significant role plasmids play in microbial evolution, there is limited knowledge of their ecology, and transfer communities. This partly due to limitations current methods associating a plasmid with its host microbiomes. To address this gap, we developed implemented novel approach identify rare hosts by combining Hi-C, proximity ligation method, enrichment for plasmid-specific DNA. We hereafter refer as Hi-C+. applied Hi-C Hi-C+ soil communities which mimicked...
The treatment of hospital effluents (HE) is a major concern, as they are suspected disseminating drugs and antibiotic resistance determinants in the environment. In order to assess HE influence on wastewater plant biomass, lab-scale conventional activated sludge systems (CAS) were continuously fed with real or urban effluent control. To gain insights into main hurdles linked treatment, we conducted multiparameter study using classical physicochemical characterization, phase contrast confocal...
Abstract Background Wastewater surveillance for SARS-CoV-2 is an emerging approach to help identify the risk of a COVID-19 outbreak. This tool can contribute public health at both community (wastewater treatment system) and institutional (e.g., colleges, prisons, nursing homes) scales. Objectives research aims understand successes, challenges, lessons learned from initial wastewater efforts colleges university systems inform future research, development implementation. Methods paper presents...
Antibiotic resistance (AR) is a threat to modern medicine, and plasmids are driving the global spread of AR by horizontal gene transfer across microbiomes environments. Determining mobile resistome responsible for this among environments essential in our efforts attenuate current crisis. Biosolids wastewater treatment plant (WWTP) byproduct used globally as fertilizer agriculture. Here, we investigated biosolids that fertilizer. This was done capturing can human pathogens commensal bacteria....
Mycoplasma ovipneumoniae is associated with respiratory disease in wild and domestic Caprinae globally, wide variation outcomes within between host species. To gain insight into phylogenetic structure mechanisms of pathogenicity for this bacterial species, we compared M. genomes 99 samples from 6 countries (Australia, Bosnia Herzegovina, Brazil, China, France USA) 4 species (domestic sheep, goats, bighorn sheep caribou). Core genome sequences assemblies goats fell two well-supported clades...
By characterizing the trajectories of antibiotic resistance gene transfer in bacterial communities such as gut microbiome, we will better understand factors that influence this spread resistance. Our aim was to investigate host network a multidrug broad-host-range plasmid culturable microbiome zebrafish. This done through
Abstract Wastewater can play a vital role in infectious disease surveillance, especially underserved communities where it reduce the equity gap to larger municipalities. However, using wastewater surveillance predictive manner remains challenge. We tested if detecting SARS-CoV-2 predict outbreaks rural communities. Under CDC National Surveillance program, we monitored several Idaho (USA). While high daily variations viral load made real-time interpretation difficult, SEIR model could factor...