A5017358738- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Antibiotic Resistance in Bacteria
- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- Microbial Community Ecology and Physiology
- Escherichia coli research studies
- CRISPR and Genetic Engineering
- DNA Repair Mechanisms
- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- Salmonella and Campylobacter epidemiology
- Pharmaceutical and Antibiotic Environmental Impacts
- Chromosomal and Genetic Variations
- Vibrio bacteria research studies
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Production and Characterization
- Bacterial biofilms and quorum sensing
- Liver Disease Diagnosis and Treatment
- Enzyme Structure and Function
- Cancer Research and Treatments
- Legume Nitrogen Fixing Symbiosis
- Algal biology and biofuel production
- Hepatitis C virus research
- Hepatitis B Virus Studies
Instituto de Biomedicina y Biotecnología de Cantabria
2015-2024
Universidad de Cantabria
2015-2024
Consejo Superior de Investigaciones Científicas
2011-2023
Instituto Tecnico Agronómico Provincial
2016-2020
Hospital Universitario 12 De Octubre
1998-2016
Sodercan
2013
Centro de Investigaciones Biológicas Margarita Salas
1999-2007
Emory University
2007
University of Washington
2005
University of California, Berkeley
2005
Horizontal gene transfer shapes the genomes of prokaryotes by allowing rapid acquisition novel adaptive functions. Conjugation allows broadest range and highest input per event. While conjugative plasmids have been studied for decades, number diversity integrative elements (ICE) in remained unknown. We defined a large set protein profiles conjugation machinery to scan over 1,000 prokaryotes. found 682 putative systems among all major phylogenetic clades showed that ICEs are most abundant...
Bacterial conjugation in Gram-negative bacteria is triggered by a signal that connects the relaxosome to coupling protein (T4CP) and transferosome, type IV secretion system. The relaxosome, nucleoprotein complex formed at origin of transfer (oriT), consists relaxase, directed nic site auxiliary DNA-binding proteins. undergoes cleavage religation during vegetative growth, but this converted unwinding reaction when competent mating pair has formed. Here, we review biochemistry relaxosomes...
Third-generation cephalosporins are a class of β-lactam antibiotics that often used for the treatment human infections caused by Gram-negative bacteria, especially Escherichia coli. Worryingly, incidence third-generation cephalosporin-resistant E. coli is increasing worldwide. Recent studies have suggested these strains, and their antibiotic resistance genes, can spread from food-producing animals, via food-chain, to humans. However, traditional typing methods, which may not provided...
Abstract Plasmids can mediate horizontal gene transfer of antibiotic resistance, virulence genes, and other adaptive factors across bacterial populations. Here, we analyze genomic composition pairwise sequence identity for over 10,000 reference plasmids to obtain a global map the prokaryotic plasmidome. in this organize into discrete clusters, which call plasmid taxonomic units (PTUs), with high average nucleotide between its members. We identify 83 PTUs order Enterobacterales , 28 them...
Conjugation of DNA through a type IV secretion system (T4SS) drives horizontal gene transfer. Yet little is known on the diversity these nanomachines. We previously found that T4SS can be divided in eight classes based phylogeny only ubiquitous protein (VirB4). Here, we use an ab initio approach to identify families systematically and specifically associated with VirB4 each class. built profiles for proteins used them scan 2262 genomes presence T4SS. Our analysis led identification thousands...
Abstract Conjugation drives the horizontal transfer of adaptive traits across prokaryotes. One-fourth plasmids encode functions necessary to conjugate autonomously, others being eventually mobilizable by conjugation. To understand evolution plasmid mobility, we studied size, gene repertoires, and conjugation-related genes. Plasmid repertoires were found vary rapidly in relation evolutionary rate relaxases, for example, most pairs with 95% identical relaxases have fewer than 50% homologs....
Genetic exchange by conjugation is responsible for the spread of resistance, virulence, and social traits among prokaryotes. Recent works unraveled functioning underlying type IV secretion systems (T4SS) its distribution recruitment other biological processes (exaptation), notably pathogenesis. We analyzed phylogeny key proteins to infer evolutionary history T4SS. show that single-stranded DNA (ssDNA) double-stranded (dsDNA) conjugation, while both based on a AAA+ ATPase, diverged before...
Tge transposon Tn21 has been transposed from R100.1 to plasmid pACYC184 and, the resulting recombinants, R388. The sites of insertion and orientation element in several pACYC184::Tn21 recombinants have examined. Restriction enzyme analysis these resulted a detailed map Tn21; this is compared with published maps relevant part R100.1. Heteroduplex shown short inverted repeat sequences at ends element. With various vitro-generated deletion mutants Tn21, internal gene necessary for transposition...
Bacterial whole genome sequence (WGS) methods are rapidly overtaking classical analysis. Many bacterial sequencing projects focus on mobilome changes, since macroevolutionary events, such as the acquisition or loss of mobile genetic elements, mainly plasmids, play essential roles in adaptive evolution. Existing WGS analysis protocols do not assort contigs between plasmids and main chromosome, thus hampering full plasmid sequences. We developed a method (called constellation networks PLACNET)...
Antimicrobial resistance is a major global health challenge. Metagenomics allows analyzing the presence and dynamics of “resistomes” (the ensemble genes encoding antimicrobial in given microbiome) disparate microbial ecosystems. However, low sensitivity specificity available metagenomic methods preclude detection minority populations (often present below their threshold) and/or identification allelic variants that differ resulting phenotype. Here, we describe novel strategy combines targeted...