A5051047014- Escherichia coli research studies
- Viral gastroenteritis research and epidemiology
- Antibiotic Resistance in Bacteria
- Salmonella and Campylobacter epidemiology
- Particle physics theoretical and experimental studies
- Clostridium difficile and Clostridium perfringens research
- High-Energy Particle Collisions Research
- Enterobacteriaceae and Cronobacter Research
- Particle Detector Development and Performance
- Bacteriophages and microbial interactions
- Vibrio bacteria research studies
- Probiotics and Fermented Foods
- Bacterial Genetics and Biotechnology
- Quantum Chromodynamics and Particle Interactions
- HIV/AIDS drug development and treatment
- Asymmetric Synthesis and Catalysis
- Listeria monocytogenes in Food Safety
- Gut microbiota and health
- Veterinary medicine and infectious diseases
- Dark Matter and Cosmic Phenomena
- Drug Solubulity and Delivery Systems
- Pharmaceutical and Antibiotic Environmental Impacts
- Carbohydrate Chemistry and Synthesis
- International Maritime Law Issues
- Synthesis and Characterization of Heterocyclic Compounds
Universidade de Santiago de Compostela
2013-2023
Health Research Institute of the Balearic Islands
2022
Centro de Investigación Biomédica en Red
2022
Universidad Complutense de Madrid
2015-2021
Instituto de Investigación Sanitaria de Santiago
2020
International Iberian Nanotechnology Laboratory
2018
Assistance Publique – Hôpitaux de Paris
2016
Inserm
2016
Université Paris Cité
2016
Délégation Paris 7
2016
Concomitant with the recent emergence of CTX-M-type extended-spectrum beta-lactamases (ESBLs), Escherichia coli has become enterobacterial species most affected by ESBLs. Multiple locales are encountering CTX-M-positive E. coli, including specifically CTX-M-15. To gain insights into mechanism underlying this phenomenon, we assessed clonality and diversity virulence profiles within an international collection CTX-M-15-positive coli.Forty-one ESBL-positive isolates from eight countries three...
Amniotic fluid was collected through an intrauterine catheter from 52 women with clinical intraamniotic infection and uninfected matched control women. The amniotic cultured quantitatively for anaerobes aerobes. Patients were the on basis of gestational age, interval membrane rupture to specimen collection, delivery. patients had a significantly higher mean temperature (38.4 vs. 37.1 C) leukocyte count (15,740 11,740 cells/mm3). In 80.6070 specimens 30.8% those subjects, ≥102 colony-forming...
Escherichia coli, particularly the adherent-invasive E. coli (AIEC) pathovar, has been increasingly implicated in ethiopathogenesis of Crohn's disease (CD). We describe richness, abundance, diversity, and pathogenic features AIEC strains that colonize intestinal mucosa. Approximately 100 colonies per biopsy from 20 CD patients (18 biopsies colon 23 ileum) 28 healthy controls (C) (25, colon; 27, were isolated. Repetitive extragenic palindrome-polymerase chain reaction (Rep-PCR) pulsed field...
ABSTRACT A total of 514 Shiga toxin-producing Escherichia coli (STEC) isolates from diarrheic and healthy cattle in Spain were characterized this study. PCR showed that 101 (20%) carried stx 1 genes, 278 (54%) possessed 2 135 (26%) both . Enterohemolysin ( ehxA ) intimin eae virulence genes detected 326 (63%) 151 (29%) the isolates, respectively. STEC belonged to 66 O serogroups 113 O:H serotypes (including 23 new serotypes). However, 67% one these 15 (O2, O4, O8, O20, O22, O26, O77, O91,...
ABSTRACT Fecal swabs obtained from 1,300 healthy lambs in 93 flocks Spain 1997 were examined for Shiga toxin-producing Escherichia coli (STEC). STEC O157:H7 strains isolated 5 (0.4%) animals 4 flocks, and non-O157 462 (36%) 63 flocks. A total of 384 ovine characterized this study. PCR showed that 213 (55%) carried the stx 1 gene, 10 (3%) possessed 2 161 (42%) both genes. Enterohemolysin ( ehxA ) intimin eae virulence genes detected 106 (28%) 23 (6%) strains, respectively. The belonged to 35...
ABSTRACT To ascertain whether on animal farms there reside extended-spectrum β-lactamase (ESBL) and plasmidic class C β-lactamase-producing Escherichia coli isolates potentially pathogenic for humans, phylogenetic analyses, pulsed-field gel electrophoresis (PFGE) typing, serotyping, virulence genotyping were performed 86 from poultry (57 isolates) pig (29 farms. E. carried genes encoding enzymes of the CTX-M-9 group as well CMY-2, whereas those mainly CTX-M-1 enzymes. Poultry differed...
ABSTRACT In order to improve the identification of avian pathogenic Escherichia coli (APEC) strains, an extensive characterization 1,491 E. isolates was conducted, based on serotyping, virulence genotyping, and experimental pathogenicity for chickens. The originated from lesions colibacillosis ( n = 1,307) or intestines healthy animals 184) France, Spain, Belgium. A subset (460 isolates) this collection defined according their chicks. Six serogroups (O1, O2, O5, O8, O18, O78) accounted 56.5%...
In this study, we have evaluated the impact of methodological approaches in determination biofilm formation by four clinical isolates Escherichia coli static assays.The assays were performed microtitre plates with two minimal and enriched broths, one- or two-steps protocol, using three different mathematical formulas to quantify adherent bacteria. Different patterns found depending on E. strain, culture medium reading optical density protocol. Strong moderate occurred mostly media. The used...
Bacterial whole genome sequence (WGS) methods are rapidly overtaking classical analysis. Many bacterial sequencing projects focus on mobilome changes, since macroevolutionary events, such as the acquisition or loss of mobile genetic elements, mainly plasmids, play essential roles in adaptive evolution. Existing WGS analysis protocols do not assort contigs between plasmids and main chromosome, thus hampering full plasmid sequences. We developed a method (called constellation networks PLACNET)...
ABSTRACT Extraintestinal pathogenic (ExPEC) Escherichia coli strains of serotype O18:K1:H7 are mainly responsible for neonatal meningitis and sepsis in humans belong to a limited number closely related clones. The same is also frequently isolated from the extraintestinal lesions colibacillosis poultry, but it not well known what extent human avian this particular related. Twenty-two ExPEC isolates origin 33 were compared on basis their virulence determinants, lethality chicks, pulsed-field...
ABSTRACT Quinolone-resistant and CTX-M-15-producing Escherichia coli isolates belonging to clone ST131 have been reported in the community. This study was designed identify these E. stools of 332 independent healthy subjects living area Paris, France. Stools were plated on media without antibiotics, order obtain dominant (Dm) fecal strain, with nalidixic acid (NAL) cefotaxime. Quinolone susceptibility, phylogenetic groups, molecular profiles, including multilocus sequence types (ST),...
To analyze whether Escherichia coli strains that cause urinary tract infections (UPEC) share virulence characteristics with the diarrheagenic E. (DEC) pathotypes and to recognize their genetic diversity, 225 UPEC were examined for presence of various properties DEC (type interaction HeLa cells, serogroups 30 genes). No correlation between adherence patterns was observed. Forty-five found, but 64% belonged one 12 (O1, O2, O4, O6, O7, O14, O15, O18, O21, O25, O75, O175) carried genes (pap,...
Extraintestinal pathogenic Escherichia coli (ExPEC) strains of serotype O1:K1:H7/NM are frequently implicated in neonatal meningitis, urinary tract infections and septicemia humans. They also commonly isolated from colibacillosis poultry. Studies to determine the similarities ExPEC different origins have indicated that avian potentially zoonotic properties. A total 59 isolates (21 colibacillosis, 15 human 23 infection septicemia) originated four countries were characterized by phylogenetic...
To evaluate the current prevalence of three clonal groups O25b:H4-B2-ST131, O15:H1-D-ST393 and CGA-D-ST69 (where ST stands for sequence type) among Escherichia coli isolates causing extraintestinal infections in Spain to characterize their virulence background, 500 consecutive non-duplicate E. were analysed.The collected during February 2009 from five hospitals different Spanish regions. Phylogenetic groups, STs, serotypes, genes, PFGE profiles, antimicrobial resistance extended-spectrum...
It is important to study commensal populations of Escherichia coli because they appear be the reservoir both extra-intestinal pathogenic E. and antibiotic resistant strains coli. We studied 279 dominant faecal from 243 adults living in community Paris area 2010. The phylogenetic group subgroup [sequence type complex (STc)] isolates presence 20 virulence genes were determined by PCR assays. O-types resistance 18 antibiotics assessed phenotypically. B2 was most frequently recovered (34.0 %),...
ABSTRACT The objectives of this study were to investigate the presence Shiga toxin-producing Escherichia coli (STEC) strains in wildlife that have spread Europe, living near human settlements; analyze their epidemiological role maintenance and transmission domestic livestock; assess potential health risk wildlife-carried strains. STEC recovered from 53% roe deer, 8.4% wild boars, 1.9% foxes sampled northwest Spain (Galicia). Of 40 serotypes identified, 21 classified as seropathotypes...
Avian pathogenic Escherichia coli (APEC) are the major cause of colibacillosis in poultry production. In this study, a total 22 E. isolated from field cases and 10 avian faecal (AFEC) were analysed. All strains characterised phenotypically by susceptibility testing molecular typing methods such as pulsed-field gel electrophoresis (PFGE) multi-locus sequence (MLST). The presence 29 virulence genes associated to APEC human extraintestinal (ExPEC) was also evaluated. For cephalosporin resistant...
We describe the epidemiology, clinical features, and molecular characterization of enterohemorrhagic Escherichia coli (EHEC) infections caused by singular hybrid pathotype O80:H2, we examine influence antibiotics on Shiga toxin production. In France, during 2005-2014, a total 54 patients were infected with EHEC O80:H2; 91% had hemolytic uremic syndrome. Two invasive infections, 2 died. All strains carried stx2 (variants stx2a, 2c, or 2d); rare intimin gene (eae-ξ); at least 4 genes...
Antimicrobial therapy is an important tool in reducing the enormous losses poultry industry caused by Escherichia coli infections (colibacillosis). However, resistance to existing antimicrobials widespread and of concern veterinarians. testing 468 avian E. strains isolated Spain showed very high levels trimethoprim-sulfamethoxazole (67%) new fluoroquinolones (13 24%). As these antimicrobial agents may cause cross-resistance with human enteric pathogens, prudent use them veterinary medicine...
We have characterized the in vitro and vivo toxic properties of cell sonic extracts from 22 animal human clinical isolates Escherichia coli that caused both necrosis rabbit skin multinucleation tissue cultures, two previously reported as being specific for E. cytotoxic necrotizing factor (CNF). Two distinct phenotypes were observed. Type 1, which was displayed by originally described CNF strains, extensive rounding cells HeLa culture assays, moderate test, absence mouse footpad test. 2, has...
Seventy-four E. coli strains isolated from piglets with diarrhea or edema disease in Spain were serotyped and examined for production of heat-labile (LT) heat-stable (ST) enterotoxins (LT-I, LT-II, STaH, STaP, STb) verotoxins (VT1, VT2, VT2v = VTe) by phenotypic (Vero cell assay infant mouse test) genotypic (colony hybridization PCR) methods. In general, an excellent correlation was found between the results obtained a PCR approach those determined biological assays. DNA probes used also...