A5021607681- Streptococcal Infections and Treatments
- Antibiotic Resistance in Bacteria
- Escherichia coli research studies
- Antimicrobial Resistance in Staphylococcus
- Orthopedic Infections and Treatments
- Pneumonia and Respiratory Infections
- Neonatal and Maternal Infections
- Bacterial Infections and Vaccines
- Enterobacteriaceae and Cronobacter Research
- Urinary Tract Infections Management
- Infectious Diseases and Tuberculosis
- Infective Endocarditis Diagnosis and Management
- Otolaryngology and Infectious Diseases
- Bacterial Genetics and Biotechnology
- Bacterial Identification and Susceptibility Testing
- Viral gastroenteritis research and epidemiology
- Antibiotics Pharmacokinetics and Efficacy
- Bacteriophages and microbial interactions
- Vibrio bacteria research studies
- Respiratory viral infections research
- Hematological disorders and diagnostics
- Salmonella and Campylobacter epidemiology
- Pharmaceutical and Antibiotic Environmental Impacts
- Pneumocystis jirovecii pneumonia detection and treatment
- Rabies epidemiology and control
Délégation Paris 7
2012-2024
Sorbonne Paris Cité
2012-2024
Université Paris Cité
2015-2024
Hôpital Robert-Debré
2015-2024
Assistance Publique – Hôpitaux de Paris
2015-2024
Inserm
2015-2024
Infection, Anti-microbiens, Modélisation, Evolution
2015-2024
Czech Academy of Sciences, Institute of Microbiology
2021
Institut Pasteur
2004-2016
Institut de Veille Sanitaire
2008-2016
The Escherichia coli species represents one of the best-studied model organisms, but also encompasses a variety commensal and pathogenic strains that diversify by high rates genetic change. We uniformly (re-) annotated genomes 20 E. strain fergusonii (the closest related species), including seven we sequenced to completion. Within approximately 18,000 families orthologous genes, found 2,000 common all strains. Although recombination are much higher than mutation rates, show, both...
ABSTRACT Inoculation of blood culture vials with joint fluid samples has revealed the important pathogenic role Kingella kingae in pediatric arthritis. However, recent studies based on broad-range 16S ribosomal DNA PCR and real-time without a probe suggest that conventional methods remain suboptimal. We developed new method is highly specific for K. applied it to collected from 89 children suspected arthritis admitted our institution during 2-year period. Real-time was also obtained before...
ABSTRACT We evaluated phage therapy in experimental infections due to S242, a fatal neonatal meningitis Escherichia coli strain belonging the worldwide-distributed O25b:H4-ST131 clone that produces extended-spectrum beta-lactamase CTX-M-15. A lytic phage, EC200 PP , active against was isolated from environmental water. After determining vitro and ex vivo stabilities pharmacokinetic properties of rat pups, we assessed therapeutic efficacy single dose 10 8 PFU using models sepsis which...
Dissemination of carbapenem resistance among Enterobacteriaceae poses a considerable threat to public health. Carbapenemase gene detection by molecular methods is the gold standard but available in only few laboratories. The aim this study was test phenotypic for metallo-β-lactamase (MBL)- or Klebsiella pneumoniae carbapenemase (KPC)-producing and associated mechanisms β-lactam against panel 30 genotypically characterized carbapenem-resistant : 9 MBL, 7 KPC, 6 OXA-48, 8 extended-spectrum...
We describe the epidemiology, clinical features, and molecular characterization of enterohemorrhagic Escherichia coli (EHEC) infections caused by singular hybrid pathotype O80:H2, we examine influence antibiotics on Shiga toxin production. In France, during 2005-2014, a total 54 patients were infected with EHEC O80:H2; 91% had hemolytic uremic syndrome. Two invasive infections, 2 died. All strains carried stx2 (variants stx2a, 2c, or 2d); rare intimin gene (eae-ξ); at least 4 genes...
ABSTRACT A new Escherichia coli virulent clonal group, O45:K1, belonging to the highly subgroup B2 1 was recently identified in France, where it accounts for one-third of E. neonatal meningitis cases. Here we describe sequence, epidemiology and function large plasmid harbored by strain S88, which is representative O45:K1 group. Plasmid pS88 133,853 bp long contains 144 protein-coding genes. It harbors three different iron uptake systems (aerobactin, salmochelin, sitABCD genes) other putative...
Invasive group A streptococcal (GAS) infections cause significant morbidity and mortality. national survey was initiated to assess the burden of invasive GAS in France, describe their clinical characteristics, molecular characteristics strains responsible for these infections. The conducted 194 hospitals, accounting 51% acute care hospital admissions France. Clinical data, predisposing factors, demographic data were obtained, all isolates emm sequence typed. We identified 664 cases...
Abstract Background The increasing incidence of community acquired infection due to Extended-Spectrum Beta-Lactamase (ESBL) -Producing Enterobacteriaceae represent a great concern because there are few therapeutic alternatives. fecal flora children in the can reservoir for ESBLs genes which located on highly transmissible plasmids and spread these among bacterial pathogens is concerning. Because intestinal carriage key factor epidemiology ESBL-producing Enterobacteriaceae, study prevalence...
Background. We aimed to describe features of Escherichia coli meningitis in a large population children and the molecular characteristics involved strains determine factors associated with severe disease or death.
Importance Henoch-Schönlein purpura (HSP) is the most common type of vasculitis in children. The factors that trigger disease are poorly understood. Although several viruses and seasonal bacterial infections have been associated with HSP, differentiating specific associations these pathogens onset HSP remains a challenge due to their overlapping patterns. Objective To analyze role epidemiology HSP. Design, Setting, Participants This cohort study comprised an interrupted time-series analysis...
ABSTRACT We studied the antimicrobial susceptibility of 322 Streptococcus pyogenes throat isolates from French children and their serotype genomic diversity. A total 22.4% were erythromycin resistant, 69.4, 4.2, 26.4% these harbored ermB , ermA mefA respectively. Increasing resistance in France is mainly associated with a few emm type 28 clones.
The genetic relatedness of 223 invasive Escherichia coli strains that cause either meningitis or urosepsis without in young infants was determined by multilocus sequence typing (MLST), ribotyping, and phylogenetic polymerase chain reaction grouping. We also the serotypes virulence genotypes (on basis 11 genes). belonged to 29 type complexes (STc), 20 ribotypes, 26 O serogroups, 39 genotypes. MLST combined with serogrouping identified 49 subtypes, "sequence types." Some types were almost...
We conducted a retrospective study comparing the presenting clinical and biologic features of 64 children who had septic arthritis caused by Kingella kingae with 26 Staphylococcus aureus. Children K. were significantly younger than those S. aureus arthritis. Otherwise, there no significant differences between 2 groups respect to fever, location, white blood cell count, synovial fluid C-reactive protein, or serum fibrinogen. However, course was better for as evidenced shorter hospitalization...
Despite the increasing recognition of Kingella kingae as an important pathogen early childhood, relative frequency and invasiveness different strains organism has not been investigated. A study was conducted to determine association K. genotypes with specific clinical syndromes temporal geographic distribution invasive clones.A collection 181 strains, isolated between 1991 2012 from Israeli patients bacteremia, skeletal system infections, or endocarditis, were typed by pulsed-field gel...
Enterohemorrhagic Escherichia coli serogroup O80, involved in hemolytic uremic syndrome associated with extraintestinal infections, has emerged France. We obtained circularized sequences of the O80 strain RDEx444, responsible for bacteremia, and noncircularized 35 E. isolated from humans animals Europe or without Shiga toxin genes. RDEx444 harbored a mosaic plasmid, pR444_A, combining virulence determinants multidrug resistance-encoding island. All strains belonged to clonal complex 165,...
We describe a new atypical Shiga-toxin-producing Escherichia coli (STEC) responsible for severe episode of haemolytic-uraemic syndrome in an adult with relapse associated bacteraemia. This STECs train serotype O80:H2 harboured stx2c and stx2d gene subtypes, the rare eae ξ variant ColV plasmid conserved virulence plasmidic region involved human avian extraintestinal pathogenic E. coli. hybrid pathotype, which represents threat, is further demonstration that STEC may be recipient factors...
The objective of this study was to evaluate the evolution and risk factors ESBL-producing Enterobacteriaceae (ESBL-E) carriage in children community for a long period distinguishing ST131 non-ST131 Escherichia coli.In prospective study, rectal samples were obtained from aged 6-24 months by paediatricians between 2010 2015. Demographic characteristics ESBL-E collected. Distribution β-lactamase genes, phylogenetic groups, virulence resistant E. coli determined.We enrolled 1886 children; 144...
Background The stability of the accuracy a diagnostic test is critical to whether clinicians can rely on its result. We aimed assess performance rapid antigen detection (RADT) for group A streptococcus (GAS) affected by clinical spectrum and/or bacterial inoculum size. Methods Throat swabs were collected from 785 children with pharyngitis in an office-based, prospective, multicenter study (2009–2010). analysed effect (i.e., McIsaac score and components) size (light or heavy GAS growth)...
Escherichia coli bacteremia in young infants may arise via either urinary tract infection or gut translocation (GT). E. GT isolates have rarely been investigated. Molecular analysis of 100 recovered from bacteremic revealed that had multilocus sequence types similar to those urosepsis but different prevalences PapGII adhesin, TcpC protectin, and ibeA invasin. Compared with late-onset isolates, early-onset were associated significantly rates the conserved virulence plasmidic region common...
We describe an outbreak of 5 osteoarticular infections among 24 daycare center attendees. Polymerase chain reaction revealed Kingella kingae in the joint fluid 1 child and 85% throat samples from healthy contacts. Multilocus sequence typing performed on carriage isolates identified unique type. Rifampin failed to eradicate K. carriage.
Background Kingella kingae, a normal component of the upper respiratory flora, is being increasingly recognized as an important invasive pathogen in young children. Genetic diversity this species has not been studied. Methods We analyzed 103 strains from different countries and clinical origins by new multilocus sequence-typing (MLST) schema. Putative virulence gene rtxA, encoding RTX toxin, was also sequenced, experimental representative assessed juvenile-rat model. Results Thirty-six...
Maternal-fetal Escherichia coli infections, such as neonatal bacteremia and meningitis, are important causes of morbidity mortality. From 2006 to 2010, we studied newborns their mothers who were colonized with E. in a French hospital order document (i) the epidemiology genetic characteristics extended-spectrum-beta-lactamase (ESBL)-producing strains, (ii) prevalence associated virulence genes, (iii) clone sequence type 131 (ST131), (iv) relationship among ESBL-producing strains. Among 2,755...