A5031597438- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Neonatal Respiratory Health Research
- Cardiac Fibrosis and Remodeling
- Connective Tissue Growth Factor Research
- Chemotherapy-induced cardiotoxicity and mitigation
- Metabolism, Diabetes, and Cancer
- Liver Diseases and Immunity
- Hepatitis Viruses Studies and Epidemiology
- Electrospun Nanofibers in Biomedical Applications
- Cancer Cells and Metastasis
- Radiomics and Machine Learning in Medical Imaging
- MicroRNA in disease regulation
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cardiac Imaging and Diagnostics
- Cardiac Ischemia and Reperfusion
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Diet and metabolism studies
- Neuroscience of respiration and sleep
- Liver Disease and Transplantation
- Cancer Genomics and Diagnostics
- Connexins and lens biology
- NF-κB Signaling Pathways
- Liver physiology and pathology
- Lung Cancer Research Studies
University Hospital of Zurich
2018-2024
University of Zurich
2023-2024
VetAgro Sup
2024
University Hospital Heidelberg
2023
National Center for Tumor Diseases
2023
German Cancer Research Center
2023
Heidelberg University
2023
Inserm
1985-2021
Institut Cochin
2016-2021
University College Cork
2009-2016
It is currently not well known how necroptosis and responses manifest in vivo. Here, we uncovered a molecular switch facilitating reprogramming between two alternative modes of signaling hepatocytes, fundamentally affecting immune hepatocarcinogenesis. Concomitant necrosome NF-κB activation which physiologically express low concentrations receptor-interacting kinase 3 (RIPK3), did lead to immediate cell death but forced them into prolonged "sublethal" state with leaky membranes, functioning...
The progression of non-alcoholic steatohepatitis (NASH) to fibrosis and hepatocellular carcinoma (HCC) is aggravated by auto-aggressive T cells. gut-liver axis contributes NASH, but the mechanisms involved consequences for NASH-induced liver cancer remain unknown. We investigated role gastrointestinal B cells in development HCC.
The effects of a chronic load nonabsorbable sugars on intracolonic bacterial metabolism carbohydrates and H2 breath excretion are disputed. However, most the discussion relies indirect evidence or results in vitro studies. Thus, we attempted to assess directly vivo lactulose oral this disaccharide. 20 g was given orally twice daily during 8 d eight normal volunteers. In all, concentration measured days 1 after ingestion morning dose. four subjects, stools were collected 2 at beginning end...
Invasive group A streptococcal (GAS) infections cause significant morbidity and mortality. national survey was initiated to assess the burden of invasive GAS in France, describe their clinical characteristics, molecular characteristics strains responsible for these infections. The conducted 194 hospitals, accounting 51% acute care hospital admissions France. Clinical data, predisposing factors, demographic data were obtained, all isolates emm sequence typed. We identified 664 cases...
The heart hosts tissue resident macrophages which are capable of modulating cardiac inflammation and function by multiple mechanisms. At present, the consequences phenotypic diversity in incompletely understood. contribution M2-polarized to resolution repair response following myocardial infarction remains be fully defined. In this study, role M2 was investigated utilising a specific CSF-1 receptor signalling inhibition strategy achieve their depletion. mice, oral administration GW2580,...
We have previously reported the cardioprotective effects of endothelial progenitor cell (EPC)-conditioned media (CM) therapy post-myocardial infarction (MI). In present study, we determined insulin-like growth factor-1 (IGF-1) contribution to EPC CM on cardiomyocyte survival, contractility, and angiogenesis in vivo. Conditioned from porcine were administered intracoronary presence absence specific neutralizing antibodies IGF-1 or control IgG a model MI. X-vivo (non-conditioned) medium was...
To extend the temporal window for cytoprotection in cardiomyocytes undergoing apoptosis after hypoxia and myocardial infarction (MI), a synthetic chemically modified mRNA (modRNA) was used to drive delivery of insulin-like growth factor-1 (IGF1) within area at risk an vivo murine model MI. Delivery IGF1 modRNA, with polyethylenimine-based nanoparticle, augmented secreted cell-associated IGF1, promoting cardiomyocyte survival abrogating cell under hypoxia-induced conditions. Translation...
<b><i>Background:</i></b> A prolonged inflammatory phase is seen in aberrant wound healing and chronic wounds. Macrophages are central to healing. Distinct macrophage subtypes have differing roles both initial inflammation later tissue repair. Broadly, these cells can be divided into M1 M2 macrophages. proliferation differentiation regulated by colony-stimulating factor 1 (CSF-1) signalling blocked GW2580, a competitive cFMS kinase inhibitor, thereby allowing for...
Interest has increased in the use of exogenous stem cells to optimize lung repair and serve as carriers a therapeutic gene for genetic airway diseases such cystic fibrosis. We investigated survival engraftment after intratracheal injection, murine model acute epithelial injury already used therapy experiments on Embryonic mesenchymal were intratracheally injected 24 hr 2% polidocanol administration, when was maximal. Stem transfected with reporter genes immediately before administration....
Because of the close interaction between tumors and immune system, immunotherapies are nowadays considered as most promising treatment against cancer. In order to define diagnosis subsequent therapy, crucial information about cells at tumor site is needed. Indeed, different types or activation status may be indicative for specific personalized treatments. Here, we present a quantitative method identify ten immuno-markers in same cut section, thereby saving precious samples enabling...
Abstract Cell‐based therapies have great potential for the treatment of cardiovascular diseases. Recently, using a transgenic mouse model Roell et al. reported that cardiac engraftment connexin43 (Cx43)‐overexpressing myoblasts in vivo prevents post‐infarct arrhythmia, common cause death patients following heart attack. We carried out similar study but clinically relevant context via transplantation autologous connexin43‐overexpressing infarcted rats. Seven days after coronary ligation, rats...
Insulin-like growth factor-1 (IGF-1) is recognized as an important regulator of cardiac structure and cardiomyocyte homeostasis. The prosurvival antiapoptotic effects IGF-1 have been investigated in vitro rodent models myocardial infarction (MI). However, the clinical application has hampered by dose-dependent side both acutely during chronic administration. We hypothesized that single, low-dose (LD-IGF-1) administered locally early reperfusion phase after acute MI a large animal model would...
Anaplastic lymphoma kinase (ALK) is a key oncogenic driver in lung adenocarcinoma patients and its fusion proteins are routinely assessed. The microfluidic tissue processor (MTP) device based on chip-confined low-volume technology allowing for rapid immunohistochemistry/immunofluorescence (IHC/IF) stainings of formalin-fixed paraffin-embedded (FFPE) or frozen samples. A novel ALK IF protocol was developed the MTP using primary mouse anti-human antibody clone 5A4. FFPE tumor whole sections...
HCC is the most common primary liver tumor, with an increasing incidence worldwide. a heterogeneous malignancy and usually develops in chronically injured liver. The NF-κB signaling network consists of canonical noncanonical branch. Activation documented. However, functional clinically relevant role its downstream effectors not established.
Hepatitis E virus (HEV) infection, one of the most common forms hepatitis worldwide, is often associated with extrahepatic, particularly renal, manifestations. However, underlying mechanisms are incompletely understood. Here, we report development a de novo immune complex-mediated glomerulonephritis (GN) in kidney transplant recipient chronic E. Applying immunostaining, electron microscopy, and mass spectrometry after laser-capture microdissection, show that GN develops parallel increasing...
Ferrets are widely used for experimental modelling of viral infections. However, background disease in ferrets could potentially confound intended interpretation. Here we report the detection a subclinical infection ferret hepatitis E virus (FRHEV) within colony sub-group female laboratory that had been enrolled on an study (non-hepatitis). Lymphoplasmacytic cuffing periportal spaces was identified histopathology but negative RNA and antigens administered virus. Follow-up metagenomic...
Malignant pleural effusion (MPE) is a severe condition of advanced tumors without effective therapy. We used digitalized immunohistochemical and transcriptional approaches to investigate the prognostic influence immune cells expression variance associated immunomodulatory molecules in MPE. Cytology tissue microarrays were constructed from MPE cell blocks 155 patients with five tumor entities. Immune lineage markers quantified by computational cytopathology on immunohistochemistry. mRNA...
Disorders affecting smooth muscle structure/function may require technologies that can generate large scale, differentiated and contractile cells (SMC) suitable for cell therapy. To date no clonal precursor population provides numbers of SMC in culture has been identified a rodent. Identification such also enhance insight into progenitor fate decisions the relationship between precursors disease states implicate SMC. In this study, we used classic expansion techniques to identify novel...
Tailored diagnostics requires immunohistochemistry (IHC) and next generation sequencing (NGS). Here we combined on a single paraffin-embedded slide microfluidic-based IHC (micro-IHC) NGS for BRAF V600E mutation detection in BRAFomas.For micro-IHC, performed the primary antibody incubation step of conventional chromogenic LabSat device (Lunaphore Technologies SA). Tumor areas immunoreactive pan-cytokeratin, pan-melanoma, mutation-specific were H-scored, microdissected, analyzed by NGS.After 2...