A5047186824- Ion Transport and Channel Regulation
- Cancer-related molecular mechanisms research
- Pancreatic function and diabetes
- Osteoarthritis Treatment and Mechanisms
- MicroRNA in disease regulation
- Caveolin-1 and cellular processes
- Reproductive System and Pregnancy
- Hormonal Regulation and Hypertension
- Genetics, Aging, and Longevity in Model Organisms
- Pluripotent Stem Cells Research
- Physiological and biochemical adaptations
- Medical Imaging and Analysis
- Cancer Cells and Metastasis
- Thyroid Disorders and Treatments
- Adipose Tissue and Metabolism
- S100 Proteins and Annexins
- Systemic Lupus Erythematosus Research
- Radiomics and Machine Learning in Medical Imaging
- Magnesium in Health and Disease
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Neuroinflammation and Neurodegeneration Mechanisms
- Muscle Physiology and Disorders
- Angiogenesis and VEGF in Cancer
- Heart Failure Treatment and Management
- CRISPR and Genetic Engineering
Southern Medical University Shenzhen Hospital
2018-2024
Marshall University
2016-2024
Second Affiliated Hospital of Fujian Medical University
2019-2024
Fujian Medical University
2019-2024
Chengdu University of Technology
2024
China Pharmaceutical University
2021
Jiangsu Food and Pharmaceutical Science College
2021
University of Toledo
2021
Sun Yat-sen University
2019
The First Affiliated Hospital, Sun Yat-sen University
2019
Abstract We report here the identification of α1 Na/K-ATPase as a major regulator proto-oncogene Src kinase and role this regulation in control Warburg effect tumor growth. Specifically, we discovered Y260 Src-specific phosphorylation binding site that is required for Src-mediated signal transduction response to number stimuli including EGF. As such, it enables dynamic aerobic glycolysis. However, such appears be lost or attenuated human cancers expression was significantly decreased...
Leptin exerts its weight-reducing effects by binding to receptor and activating signal transduction in hypothalamic neurons other cell types. To identify the components of leptin pathway, an approach was developed which bacterially expressed phosphorylated fragments Ob b (Ob-Rb) were used as affinity agents. Ob-Rb form leads tyrosyl phosphorylation cytoplasmic domain receptor. Two three tyrosines Ob-Rb, at positions 985 1138, are after treatment. Affinity chromatography using a...
Thyroid hormone controls remodeling of the tadpole intestine during climax amphibian metamorphosis. In 8 days, Xenopus laevis shortens in length by 75%. Simultaneously, longitudinal muscle fibers contract about same extent. The radial also shorten as diameter narrows. Many undergo programmed cell death. We conclude that and contraction play key roles shortening process. Shortening is accompanied a temporary “heaping” epithelial cells into many layers at climax. Cells face lumen apoptosis. By...
Two important features of amphibian metamorphosis are the sequential response tissues to different concentrations thyroid hormone (TH) and development negative feedback loop between pituitary gland that regulates TH synthesis by gland. At climax in Xenopus laevis (when level is highest), ratio circulating precursor thyroxine (T 4 ) active form 3,5,3′-triiodothyronine 3 blood many times higher than it tissues. This difference because conversion T target cells tadpole catalyzed enzyme type II...
Diabetes was an increasing public health problem nowadays. Accumulating evidences had shed a light on the involvement of endothelial cell dysfunction in pathogenesis diabetes-associated vascular diseases. MiR-21, multiple-functional miRNA, evidenced to be involved dysfunction, however, underlying molecular mechanisms were still unknown. In current study, we investigated intrinsic link between miR-21 and high glucose-induced dysfunction. We demonstrated that expression elevated circulating...
A close relationship between cancer progression and microRNAs (miRNAs) regulation has been demonstrated. Abnormal microRNA-206 (miR-206) expression shown to be related the development of malignancies. However, role miR-206 in hepatocellular carcinoma (HCC) remains unclear. Here, we evaluated function HCC. Results showed that was decreased 27 human HCC tissues compared with adjacent normal tissues. Conversely, cMET up-regulated tissues, levels were negatively correlated expression. Abnormally...
Thyroid hormone (TH) is required for limb development in Xenopus laevis . Specific cell types the growing were targeted expression of a dominant negative form TH receptor by sperm-mediated transgenesis. Limb muscle development, innervation from spinal cord, and cartilage growth can be inhibited without affecting patterning or differentiation other types. Remodeling skin occurs late metamorphosis after has formed. The coordination these independent programs affected part control that exerts...
Pelvic bone tumors represent a harmful orthopedic condition, encompassing both benign and malignant forms. Addressing the issue of limited accuracy in current machine learning algorithms for tumor image segmentation, we have developed an enhanced segmentation algorithm. This algorithm is built upon improved full convolutional neural network, incorporating fully network (FCNN-4s) conditional random field (CRF) to achieve more precise segmentation. The was employed conduct initial on...
During premetamorphic stages, Xenopus laevis tadpoles expressing either a dominant-negative thyroid hormone (TH) receptor or type-III iodothyronine deiodinase transgene in the nervous system have reduced TH-induced proliferation spinal cord and produce fewer hindlimb-innervating motorneurons. prometamorphic innervation of hindlimbs is reduced, few functional neuromuscular connections are formed. By metamorphic climax, limb movement impaired, ranging from uncoordinated leg swimming to...
BACKGROUND ELL-associated factor 2 (EAF2) is an androgen-regulated tumor suppressor in the prostate. However, mechanisms underlying suppressive function of EAF2 are still largely unknown. Identification factors capable modulating will help elucidate function. METHODS Using eaf-1(the ortholog EAF2) mutant C. elegans model, RNAi screen was used to identify on basis their knockdown synergistically enhance reduced fertility phenotype eaf-1 elegans. In human cells, interaction with FOXA1 and...
Abstract Background Spinal cord injury (SCI) triggers the primary mechanical and secondary inflammation-mediated injury. Neuroinflammation-mediated insult causes extensive neurological damage after SCI. Microglia play a pivotal role in initiation progression of post-SCI neuroinflammation. Methods To elucidate significance LRCH1 to microglial functions, we applied lentivirus-induced knockdown microglia culture tested microglia-mediated inflammatory reaction both vitro rat SCI model. Results...
Abstract Aim Highly prevalent diseases such as insulin resistance and heart failure are characterized by reduced metabolic flexibility reserve. We tested whether Na/K‐ATPase (NKA)‐mediated regulation of Src kinase, which requires two NKA sequences specific to the α1 isoform, is a regulator capacity that can be targeted pharmacologically. Methods Metabolic was challenged functionally Seahorse flux analyses glucose deprivation in LLC‐PK1‐derived cells expressing binding rat α1, non‐Src‐binding...
Long non-coding RNA (lncRNA) plasmacytoma variant translocation 1 (PVT1) and growth arrest specific 5 (GAS5) have opposite functions in the apoptosis of chondrocytes, which are involved pathogenesis osteoarthritis (OA). The roles PVT1 GAS5 OA may indicate existence crosstalk between them OA. This study aimed to explore possible interaction Accumulation control synovial fluid samples was measured by RT-qPCR. chondrocytes explored overexpression experiments. Dual-luciferase reporter assay...
Abstract Background： Single-cell RNA sequencing (scRNA-seq) was recently adopted for exploring molecular programmes and lineage progression patterns of pathogenesis important diseases. In this study, we use scRNA-seq to identify potential markers chondrocytes in osteoarthritis (OA) explore the function different types OA. Methods： Here aimed identifies biomarkers differentiation chondrocyte by seq analysis. GO KEGG analysis were used prove candidate marker genes . Protein–protein interaction...