A5078268612- Ion Transport and Channel Regulation
- Magnesium in Health and Disease
- Caveolin-1 and cellular processes
- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Respiratory viral infections research
- Monoclonal and Polyclonal Antibodies Research
- Pancreatic function and diabetes
- Herpesvirus Infections and Treatments
- Influenza Virus Research Studies
- Virology and Viral Diseases
- Ion channel regulation and function
- Marine and coastal ecosystems
- Animal Virus Infections Studies
- Diet and metabolism studies
- Marine Biology and Environmental Chemistry
- Renal and related cancers
- Effects and risks of endocrine disrupting chemicals
- Immunodeficiency and Autoimmune Disorders
- Invertebrate Immune Response Mechanisms
- Plant nutrient uptake and metabolism
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Hydrogen's biological and therapeutic effects
- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
Shanghai Ocean University
2020-2025
Ministry of Agriculture and Rural Affairs
2020-2024
Tianjin University
2024
Dalian Ocean University
2022-2024
Institute of Psychology, Chinese Academy of Sciences
2020-2024
University of Chinese Academy of Sciences
2024
Shanghai Zhangjiang Laboratory
2022-2023
Huazhong Agricultural University
2022-2023
Key Laboratory of Guangdong Province
2022-2023
Agricultural Genomics Institute at Shenzhen
2022-2023
Abstract We report here the identification of α1 Na/K-ATPase as a major regulator proto-oncogene Src kinase and role this regulation in control Warburg effect tumor growth. Specifically, we discovered Y260 Src-specific phosphorylation binding site that is required for Src-mediated signal transduction response to number stimuli including EGF. As such, it enables dynamic aerobic glycolysis. However, such appears be lost or attenuated human cancers expression was significantly decreased...
Abstract Emerging evidence indicates that modulating glutathione peroxidase 4 (GPX4) to induce ferroptosis is a promising strategy for tumor treatment. However, most of the GPX4 small molecule inhibitors face limitations due their poor delivery efficacy and low specificity activation. Herein, ferroptosis‐inducing nanomedicine developed integrates nutlin‐3 with iridium oxide nanoclusters (NUT‐IrO x NCs) enhanced ferroptosis‐driven multimodal therapeutic in colorectal cancer (CRC). This...
Na-K-ATPase is a fundamental component of ion transport. Four α isoforms the catalytic subunit are expressed in human cells. The ubiquitous α1 was recently discovered to also mediate signal transduction through Src kinase. In contrast, α2 expression limited few cell types including myocytes, where it coupled Na + /Ca 2+ exchanger. To test whether rat capable cellular signaling like its counterpart recipient mammalian system, we used an knockdown pig renal epithelial (PY-17) create...
DNA double-strand breaks (DSBs) are the most toxic form of damage in cells. Homologous recombination (HR) is an error-free repair mechanism for DSBs as well a basis gene targeting using genome-editing techniques. Despite importance HR, HR plants poorly understood. Through genetic screens response mutants (DDRMs), we find that Arabidopsis ddrm2 mutant hypersensitive to DSB-inducing reagents. DDRM2 encodes protein with four BRCA1 C-terminal (BRCT) domains and highly conserved including...
Of the four Na-K-ATPase α-isoforms, ubiquitous α1 possesses both ion transport and Src-dependent signaling functions. Mechanistically, we have identified two putative pairs of domain interactions between Src that are critical for function. Our subsequent report α2 lacks these Src-binding sites fails to carry on further supported our proposed model direct interaction but fell short providing evidence a causative role. This hypothesis was specifically tested here by introducing key residues...
Mice may share similar mechanism with human underlying reproductive toxicity induced by di(2-ethylhexyl) phthalate (DEHP), which is not supposed to be associated decreased testicular testosterone. Pregnant mice were exposed DEHP gavage, the dosage regime beginning at relevant exposure level. After in utero exposure, loss of Sertoli cells and germ observed male pups postnatal days 21. And SRY-related HMG box 9 (SOX9), Fibroblast growth factor-9 (FGF9), Double-sex Mab-3 related transcripttion...
Abstract Aim Highly prevalent diseases such as insulin resistance and heart failure are characterized by reduced metabolic flexibility reserve. We tested whether Na/K‐ATPase (NKA)‐mediated regulation of Src kinase, which requires two NKA sequences specific to the α1 isoform, is a regulator capacity that can be targeted pharmacologically. Methods Metabolic was challenged functionally Seahorse flux analyses glucose deprivation in LLC‐PK1‐derived cells expressing binding rat α1, non‐Src‐binding...
Background: Mumps, a communicable, acute and previously well-controlled disease, has had recent occasional resurgences in some areas.Methods: A randomized, double-blind, controlled multistep phase I study of an F-genotype attenuated mumps vaccine produced human diploid cells was conducted. total 300 subjects were enrolled divided into 4 age groups: 16–60 years, 5–16 2–5 years 8–24 months. The groups immunized with one injection per subject. Three different doses the vaccine, (3.5 ± 0.25...
DNA replication stress threatens genome stability and affects plant growth development. How plants resolve is poorly understood. The protein kinase WEE1-mediated cell cycle arrest required for responses. E3 ubiquitin ligases anaphase-promoting complex/cyclosome (APC/C) Skp1/Cullin 1/F-box (SCF) are essential regulators of the cycle. Here, we show that APC/CCDC20 mediates degradation SCFFBL17 during responses in Arabidopsis thaliana. Biochemically, WEE1 interacts with phosphorylates APC/C...
Two full-length cDNA (Pt-CHH1 and Pt-CHH2) sequences encoding crustacean hyperglycemic hormone (CHH) were cloned from tissues of the swimming crab (Portunus trituberculatus) using RACE. Pt-CHH1 was eyestalk, whereas Pt-CHH2 thoracic ganglia. Sequence structure analyses suggest that they may be generated alternative splicing. Tissue distribution showed transcript only detected in while observed several extra-eyestalk tissues. The levels during molting ovarian development determined qPCR. In...
Recent studies have highlighted a critical role for CD40 in the pathogenesis of renal injury and fibrosis. However, little is currently understood about regulation this setting.We use novel Na/K-ATPase cell lines inhibitors order to demonstrate regulatory function with regards expression function. We utilize 5/6 partial nephrectomy as well direct infusion ligand mechanism exists vivo.We that knockdown α1 isoform causes reduction while rescue but not α2 restores epithelial cells. Second,...
Tris (1,3-dichloro-2-propyl) phosphate (TDCPP) is a growing concern and may be potential risk to marine environmental health due its widespread usage distribution. However, the toxic effects of TDCPP on cardiac development in fish have not been reported. In this study, Oryzias melastigma embryos were exposed at doses 0, 0.04, 0.4, 4 40 μg/L from early embryogenesis 10 days postfertilization (dpf). Then, heart rate sinus venosus–bulbus arteriosus (SV–BA) distance measured 5, 6, 8 dpf....
Several signaling events have been recognized as essential for regulating cell lineage specification and organogenesis in animals. We find that the gain of an amino-terminal caveolin binding motif (CBM) α subunit Na/K-adenosine triphosphatase (ATPase) (NKA) is required early stages both mice Caenorhabditis elegans. The evolutionary CBM occurred at same time acquisition sites Na+/K+. Loss this does not affect or initiation organogenesis, but arrests further organ development. Mechanistically,...
Abstract The multilocular trait is generally considered as a desirable agricultural for the high‐yield breeding of Brassica crops and has attracted considerable attention. siliques plants contain more chambers seeds than those bilocular rapeseed varieties and, thus, have higher yield. Furthermore, are precious germplasm resources crops. In this article, we provide summary researches regarding in rapeseed, including its natural germplasms, silique morphology, genetic models, regulatory...
Mumps vaccine immunizations have reduced the incidence of this disease. With variation mumps circulating strain, novel strains are always important.A 2-center parallel, randomized, double-blind noninferiority trial was performed to compare an F-genotype attenuated (SP strain) A-genotype (S-79, Jeryl-Lynn in 1080 healthy children aged 8-24 months Hubei, China.Participants were randomly assigned receive a high or low dose SP S79 and then assessed clinically at 30 minutes 1-28 days...