A5033323668- Ion Transport and Channel Regulation
- Connexins and lens biology
- Barrier Structure and Function Studies
- Ion channel regulation and function
- Drug Transport and Resistance Mechanisms
- Pancreatic function and diabetes
- Ion Channels and Receptors
- Caveolin-1 and cellular processes
- Wnt/β-catenin signaling in development and cancer
- Heme Oxygenase-1 and Carbon Monoxide
- Protease and Inhibitor Mechanisms
- Genetic and Kidney Cyst Diseases
- Hedgehog Signaling Pathway Studies
- Iron Metabolism and Disorders
- Cellular transport and secretion
- Biochemical Analysis and Sensing Techniques
- S100 Proteins and Annexins
- Hearing, Cochlea, Tinnitus, Genetics
- Cancer-related molecular mechanisms research
- Renal and related cancers
- Photoreceptor and optogenetics research
- Cell Adhesion Molecules Research
- Cardiovascular and exercise physiology
- Neuroscience and Neuropharmacology Research
- Hepatitis B Virus Studies
Marshall University
2016-2023
Center for Research and Advanced Studies of the National Polytechnic Institute
2007-2016
Instituto Politécnico Nacional
2011
Center for Neurosciences
2009-2010
Centro de Investigación en Materiales Avanzados
2006
Epithelial cells treated with high concentrations of ouabain (e.g., 1 μM) retrieve molecules involved in cell contacts from the plasma membrane and detach one another their substrates. On basis this observation, we suggested that might also modulate at low, nontoxic levels (10 or 50 nM). To test possibility, analyzed its effect on a particular type cell–cell contact: tight junction (TJ). We demonstrate neither inhibit K + pumping nor disturb balance cell, modulates degree sealing TJ as...
Cell adhesion is a crucial step in proliferation, differentiation, migration, apoptosis, and metastasis. In previous works we have shown that cell modulated by ouabain, highly specific inhibitor of Na+,K+-ATPase, recently found to be hormone. the present work pursue investigation effect ouabain on special type cell-cell interaction: rescue ouabain-sensitive MDCK cells (W) ouabain-resistant (R). cultured monolayers pure W cells, triggers "P-->A mechanism" (from pump/adhesion) consisting...
The very existence of higher metazoans depends on the vectorial transport substances across epithelia. A crucial element this is membrane enzyme Na(+),K(+)-ATPase. Not only distributed in a polarized manner restricted domain plasma but also it creates ionic gradients that drive net movement glucose, amino acids, and ions entire epithelium. In previous work, we have shown Na(+),K(+)-ATPase polarity interactions between beta subunits Na(+),K(+)-ATPases located neighboring cells these anchor at...
The exchange of substances between higher organisms and the environment occurs across transporting epithelia whose basic features are tight junctions (TJs) that seal intercellular space, polarity, which enables cells to transport vectorially. In a previous study, we demonstrated 10 nM ouabain modulates TJs, now show it controls polarity as well. We gauge through development cilium at apical domain Madin-Darby canine kidney (MDCK, epithelial dog kidney). Ouabain accelerates ciliogenesis in an...
The Na/K-ATPase α1 polypeptide supports both ion-pumping and signaling functions. α3 differs from in its primary structure tissue distribution. expression of seems particularly important neurons, recent clinical evidence a unique role this isoform normal brain function. nature specific has remained elusive, because the ubiquitous presence hindered efforts to characterize α3-specific functions mammalian cell systems. Using knockdown pig kidney cells (PY-17), we generated first stable line...
Epithelia can adjust the permeability of paracellular permeation route by regulating degree sealing tight junction. This is reflected a transepithelial electrical resistance (TER) ranging from few tenths to several thousand ohms times square centimeters, depending on difference in composition between fluid lumen and interstitial fluid. Although teleologically sound, such correlation requires physiological explanation. We have previously shown that urine extracts different animal species...
The distribution of Na/K-ATPase α-isoforms in skeletal muscle is unique, with α1 as the minor (15%) isoform and α2 comprising bulk pool. acute isoform-specific role performance resistance to fatigue well known, but has not been thoroughly investigated. In vitro, we reported that a promoting cell growth supported by α2. To assess whether serves this trophic muscle, used α1-haploinsufficient (α1+/-) mice. A 30% decrease protein expression without change induced modest yet significant 10%...
The finding that endogenous ouabain acts as a hormone prompted efforts to elucidate its physiological function. In previous studies, we have shown 10 nM (i.e., concentration within the range) modulates cell-cell contacts such tight junctions and apical/basolateral polarity. this study, examined whether affects another important feature: gap junction communication (GJC).We employed two different approaches: 1) analysis of cell-to-cell diffusion neurobiotin injected into particular MDCK cell...
The fact that ouabain has been identified as an endogenous substance, led us to inquire its physiological role in epithelial cells. Based on previous observations, we hypothesized it influences processes related cell contacts. Previously have shown nanomolar concentrations of up-regulate tight junctions, accelerate ciliogenesis, and increase gap junctional intercellular communication (GJIC). Given silencing assays indicated connexin 43 (Cnx43) is involved the GJIC response, present work...
We have reported that the reduction in plasma membrane cholesterol could decrease cellular Na/K-ATPase α1-expression through a Src-dependent pathway. However, it is unclear whether regulate other α-isoforms and molecular mechanisms of this regulation are not fully understood. Here we used cells expressing different α isoforms found by U18666A decreased expression α1-isoform but α2- or α3-isoform. Imaging analyses showed redistribution α1 α3 α2. Moreover, led to late endosomes/lysosomes,...
At 10 nM, ouabain elicits changes in cell contacts, which are independent and usually opposite direction to effects occurring at µM levels, suggesting that these depend on entirely different mechanisms.1 However, this does not discard the possibility both instances would act same receptor. We demonstrate such is case by comparing response of wild ouabain-resistant MDCK cells a very special type contact, tight junction (TJ).
Ouabain, a well-known plant-derived toxin, is also hormone found in mammals at nanomolar levels that binds to site located the a-subunit of Na⁺,K⁺-ATPase. Our main goal was understand physiological roles ouabain. Previously, we ouabain increases degree tight junction sealing, GAP junction-mediated communication and ciliogenesis. Considering our previous results, investigated effect on adherens junctions.We used immunofluorescence immunoblot methods measure 10 nM cellular nuclear content...
Several signaling events have been recognized as essential for regulating cell lineage specification and organogenesis in animals. We find that the gain of an amino-terminal caveolin binding motif (CBM) α subunit Na/K-adenosine triphosphatase (ATPase) (NKA) is required early stages both mice Caenorhabditis elegans. The evolutionary CBM occurred at same time acquisition sites Na+/K+. Loss this does not affect or initiation organogenesis, but arrests further organ development. Mechanistically,...
Abstract TRPV4 is a nonselective cationic channel responsive to several physical and chemical stimuli. Defects in function result human diseases, such as skeletal dysplasias, arthropathies, peripheral neuropathies. Nonetheless, little known about the role of other cellular functions, nuclear Ca 2+ homeostasis or ‐regulated transcription. Here, we confirmed presence full‐length nuclei nonpolarized Madin‐Darby canine kidney cells. Confocal imaging showed that activation increases cytoplasmic...
Ouabain, a substance originally obtained from plants, is now classified as hormone because it produced endogenously in certain animals, including humans. However, its precise effects on the body remain largely unknown. Previous studies have shown that ouabain can influence phenotype of epithelial cells by affecting expression cell-cell molecular components and voltage-gated potassium channels. In this study, we conducted whole-cell clamp assays to determine whether affects activity and/or...
Despite advances in cancer treatment, drug resistance and metastasis continue to contribute treatment failure. Since are features that often occur toward the late stages disease after withstanding numerous selective pressures, they may rely on a shared adaptive mechanism order persist. The heat shock response is one of most well conserved responses cellular stress found nature. A major player HSP90, with some studies suggesting it can facilitate molecular evolution cancer. Non-small cell...