A5005124032- Ion Channels and Receptors
- Barrier Structure and Function Studies
- Ion channel regulation and function
- Connexins and lens biology
- RNA Interference and Gene Delivery
- Ion Transport and Channel Regulation
- Glycosylation and Glycoproteins Research
- Cellular transport and secretion
- Corneal Surgery and Treatments
- Nerve injury and regeneration
- RNA and protein synthesis mechanisms
- Neuroscience and Neuropharmacology Research
- Autism Spectrum Disorder Research
- Mechanical Circulatory Support Devices
- Organ Transplantation Techniques and Outcomes
- Advanced Proteomics Techniques and Applications
- RNA regulation and disease
- Nicotinic Acetylcholine Receptors Study
- Advanced Glycation End Products research
- Nuclear Receptors and Signaling
- Cardiac electrophysiology and arrhythmias
- Caveolin-1 and cellular processes
- Free Radicals and Antioxidants
- 3D Printing in Biomedical Research
- Ocular Surface and Contact Lens
Instituto Politécnico Nacional
2009-2024
Center for Research and Advanced Studies of the National Polytechnic Institute
2009-2024
Instituto de Estudios Avanzados
2007
Centro de Investigación en Materiales Avanzados
1996
Centre of Advanced Studies
1993-1995
The neurotrophin Brain-Derived Neurotrophic Factor (BDNF) influences nigral dopaminergic neurons via autocrine and paracrine mechanisms. reduction of BDNF expression in Parkinson's disease substantia nigra (SN) might contribute to the death because inhibiting SN causes parkinsonism rat. This study aimed demonstrate that increasing rats with one week 6-hydroxydopamine lesion recovers from parkinsonism. plasmids phDAT-BDNF-flag phDAT-EGFP, coding for enhanced green fluorescent protein, were...
The Transient Receptor Potential Vanilloid 4 (TRPV4) channel has been shown to function in many physiological and pathophysiological processes. Despite abundant information on its importance physiology, very few endogenous agonists for this have described, underlying mechanisms activation clarified. TRPV4 is expressed by several types of cells, such as vascular endothelial, skin lung epithelial where it plays pivotal roles their function. In the present study, we show that activated...
ABSTRACT MDCK (epithelial cells from the dog kidney) plated at confluence, establish tight junctions in 12-15 hours through a process that requires protein synthesis, formation of ring actin filaments close contact with lateral membrane cells, calmodulin, and Ca2+-dependent exocytic fusion junction (TJ)-associated components. Monolayers incubated absence Ca2+ make no TJs. Yet, if is added under these circumstances, TJs are made faster kinetics. needed mainly site located on outer side cell...
Recently we showed that the neurotensin polyplex is a nanoparticle carrier system targets reporter genes in nigral dopamine neurons vivo. Herein, report its first practical application experimental parkinsonism, which consisted of transfecting with gene coding for human glial cell line-derived neurotrophic factor (hGDNF). Hemiparkinsonism was induced rats by single dose 6-hydroxydopamine (30 microg) into ventrolateral part striatum. We transfection hGDNF substantia nigra 1 week after...
TRPV4 (transient receptor potential vanilloid 4) is a cation channel activated by hypotonicity, moderate heat, or shear stress. We describe the expression of during differentiation corneal epithelial cell model, RCE1(5T5) cells. late feature that concentrated in apical membrane outmost layer stratified epithelia. Ca2+ imaging experiments showed activation with GSK1016790A produced an influx calcium was blunted specific blocker RN-1734. analyzed involvement measuring development...
The finding that endogenous ouabain acts as a hormone prompted efforts to elucidate its physiological function. In previous studies, we have shown 10 nM (i.e., concentration within the range) modulates cell-cell contacts such tight junctions and apical/basolateral polarity. this study, examined whether affects another important feature: gap junction communication (GJC).We employed two different approaches: 1) analysis of cell-to-cell diffusion neurobiotin injected into particular MDCK cell...
Abstract Pax‐6 is a regulatory gene with major role during visual system development, but its association corneal epithelial differentiation not clearly established. Using the RCE1‐(5T5) cell line, which mimics differentiation, we analyzed biological role. Immunostaining of proliferating colonies and confluent sheets showed that Pax‐6‐positive cells were also K3 keratin‐positive, suggesting expressed in differentiating cells. mRNA was barely early cultures; after confluence, levels raised up...
// Stephan Arni 1, * , Thi Hong Nhung Le Rik de Wijn 2 Refugio Garcia-Villegas 3 Martjin Dankers Walter Weder 1 and Sven Hillinger Department of Thoracic Surgery, University Hospital Zürich, Switzerland PamGene International B.V., 's-Hertogenbosch, The Netherlands Physiology, Biophysics Neuroscience, Centro Investigación y Estudios Avanzados del IPN, Mexico City, Equal contribution as co-first authors, work was performed at the Correspondence to: Arni, email: stephan.arni@usz.ch Keywords:...
Abstract Na x is a sodium channel, thought to be descendant of the voltage‐gated channel family. Nevertheless, not activated by voltage but rather augmentation extracellular over 150 mM. In brain, it localized circumventricular organs, important regions for salt and water homeostasis in mammals, where operates as sodium‐level sensor body fluid. expressed lung, uterus, heart, also found trigeminal dorsal root ganglia nonmyelinating Schwann cells, its physiological role remains unclarified....
Abstract Polycystic kidney disease (PKD), a characterized by the enlargement of through cystic growth is fourth leading cause end-stage world-wide. Transient receptor potential Vanilloid 4 (TRPV4), calcium-permeable TRP, channel participates in cell physiology and since TRPV4 forms complexes with another whose malfunction associated to PKD, TRPP2 (or PKD2), we sought determine whether patients exhibit previously unknown mutations TRPV4. Here, report presence gene diagnosed PKD that they...
Dp71 dystrophin is the main DMD gene product expressed in central nervous system. Experiments using PC12 cells as a neuronal model have shown that isoforms are involved differentiation, adhesion, cell division, and nuclear architecture. To contribute to knowledge of domains function, we previously reported isolation partial characterization Dp71[INCREMENT]78–79 (a mutant lacks exons 71, 78, 79), which stimulates differentiation PC12-C11 clone. In this article, generated doxycycline...
Abstract TRPV4 is a nonselective cationic channel responsive to several physical and chemical stimuli. Defects in function result human diseases, such as skeletal dysplasias, arthropathies, peripheral neuropathies. Nonetheless, little known about the role of other cellular functions, nuclear Ca 2+ homeostasis or ‐regulated transcription. Here, we confirmed presence full‐length nuclei nonpolarized Madin‐Darby canine kidney cells. Confocal imaging showed that activation increases cytoplasmic...