A5077461434- Barrier Structure and Function Studies
- Connexins and lens biology
- Cancer-related molecular mechanisms research
- S100 Proteins and Annexins
- Neurological diseases and metabolism
- Wnt/β-catenin signaling in development and cancer
- Hippo pathway signaling and YAP/TAZ
- Neurological Disease Mechanisms and Treatments
- Ion Transport and Channel Regulation
- Cell Adhesion Molecules Research
- Hedgehog Signaling Pathway Studies
- Cellular transport and secretion
- Ion channel regulation and function
- Pancreatic function and diabetes
- Plant-based Medicinal Research
- Amoebic Infections and Treatments
- Cancer-related gene regulation
- Glycosylation and Glycoproteins Research
- Trace Elements in Health
- Lipid Membrane Structure and Behavior
- Parasitic Infections and Diagnostics
- Pregnancy and preeclampsia studies
- Drug Transport and Resistance Mechanisms
- Microfluidic and Bio-sensing Technologies
- Neonatal and fetal brain pathology
Center for Research and Advanced Studies of the National Polytechnic Institute
2014-2024
Instituto Politécnico Nacional
2002-2022
Institute of Molecular Biology and Biophysics
2008-2013
Tecnológico Nacional de México
2013
Laboratoire de physiologie cellulaire et moléculaire
2002
Université Côte d'Azur
2002
The University of Texas Medical Branch at Galveston
1996
Centro de Investigación en Materiales Avanzados
1983-1995
Mexican Social Security Institute
1995
Centre of Advanced Studies
1989-1995
Extracellular Ca2+ triggers assembly and sealing of tight junctions (TJs) in MDCK cells. These events are modulated by G-proteins, phospholipase C, protein kinase C (PKC), calmodulin. In the present work we observed that 1,2-dioctanoylglycerol (diC8) promotes TJ low extracellular Ca2+, as evidenced translocation TJ-associated ZO-1 to plasma membrane, formation junctional fibrils freeze-fracture replicas, decreased permeability intercellular space [3H]mannitol, reorganization actin filaments...
Upon transferring confluent monolayers of Madin-Darby canine kidney (MDCK) cells from a low-Ca2+ medium (1-5 microM) to one with 1.8 mM Ca2+ (Ca switch), tight junctions (TJs) assemble and seal, transepithelial electrical resistance (TER) develops in 4-5 h, presumably through exocytotic fusion that incorporates junctional components the surface membrane. In present work we test this possibility observe 1) Ca switch raises cytosolic concentration ion; 2) it also increases membrane area by...
Rotaviruses constitute a major cause of diarrhea in young mammals. utilize different integrins as cell receptors, therefore upon their arrival to the intestinal lumen integrin receptors will be hidden below tight junction (TJ), on basolateral membrane. Here we have studied whether rotavirus outer capsid proteins are capable opening paracellular space sealed by TJ. From outermost layer rotavirus, 60 spikes formed protein VP4 projected. is essential for virus-cell interactions and cleaved...
Clinical manifestations of coronavirus disease 2019 (COVID-19) in pregnant women are diverse, and little is known the impact on placental physiology. Severe acute respiratory syndrome (SARS-CoV-2) has been detected human placenta, its binding receptor ACE2 present a variety cells, including endothelium. Here, we analyze COVID-19 endothelium, studying by immunofluorescence expression von Willebrand factor (vWf), claudin-5, vascular endothelial (VE) cadherin decidua chorionic villi placentas...
Recent reports have indicated the participation of tight junction (TJ) proteins in regulation gene expression and cell proliferation. Here, we studied role zona occludens (ZO)-2, a TJ peripheral protein, cyclin D1 transcription. We found that ZO-2 down-regulates transcription dose-dependent manner. To understand how represses promoter activity, used deletion analyses negatively regulates via an E box it diminishes Because does not associate directly with DNA, electrophoretic mobility shift...
Here, we have studied the effect of tight junction protein zona occludens (ZO)-2 on cyclin D1 (CD1) expression. CD1 is essential for cell progression through G1 phase cycle. We found that in cultures synchronized Madin-Darby canine kidney cells, ZO-2 inhibits proliferation at G0/G1 and decreases level. These effects occur response to a diminished translation an augmented degradation proteosome triggered by ZO-2. overexpression amount Glycogen synthase kinase-3beta phosphorylated Ser9...
Transit of human neural stem cells, ReNcell CX, through the blood brain barrier (BBB) was evaluated in an vitro model BBB and nude mice. The based on rat microvascular endothelial cells (RBMECs) cultured Millicell inserts bathed from basolateral side with conditioned media (CM) astrocytes or glioma C6 cells. Glioma CM induced a significant transendothelial migration ReNcells CX comparison to astrocyte CM. presence high amounts HGF, VEGF, zonulin PGE2, together low abundance EGF, promoted...
Renal compensatory hypertrophy (RCH) restores normal kidney function after disease or loss of tissue and is characterized by an increase in organ size due to cell enlargement not proliferation. In MDCK renal epithelial cells, silencing the tight junction protein zona occludens 2 (ZO-2 KD) induces two mechanisms: prolonging time that cells spend at G1 phase cycle cyclin D1 level, augmenting rate synthesis. The latter triggered nuclear accumulation increased transcriptional activity...
The cytotrophoblast of human placenta transitions into an outer multinucleated syncytiotrophoblast (STB) layer that covers chorionic villi which are in contact with maternal blood the intervillous space. During pregnancy, Zika virus (ZIKV) poses a serious prenatal threat. STB cells resistant to ZIKV infections, yet placental within mesenchyme targets infection. We seek determine whether can open paracellular pathway cells. This route is regulated by tight junctions (TJs) present uppermost...
ABSTRACT MDCK (epithelial cells from the dog kidney) plated at confluence, establish tight junctions in 12-15 hours through a process that requires protein synthesis, formation of ring actin filaments close contact with lateral membrane cells, calmodulin, and Ca2+-dependent exocytic fusion junction (TJ)-associated components. Monolayers incubated absence Ca2+ make no TJs. Yet, if is added under these circumstances, TJs are made faster kinetics. needed mainly site located on outer side cell...