A5083892884- Wnt/β-catenin signaling in development and cancer
- Cancer-related gene regulation
- Barrier Structure and Function Studies
- RNA Research and Splicing
- Kruppel-like factors research
- Cancer-related molecular mechanisms research
- Connexins and lens biology
- Ubiquitin and proteasome pathways
- 3D Printing in Biomedical Research
- Skin and Cellular Biology Research
- Genetics and Neurodevelopmental Disorders
- S100 Proteins and Annexins
- Peptidase Inhibition and Analysis
- Cancer-related Molecular Pathways
- Helicobacter pylori-related gastroenterology studies
- Epigenetics and DNA Methylation
- Monoclonal and Polyclonal Antibodies Research
- Cell Adhesion Molecules Research
- Immune Response and Inflammation
- Microtubule and mitosis dynamics
- RNA modifications and cancer
- Immune cells in cancer
- Galectins and Cancer Biology
- Signaling Pathways in Disease
- DNA Repair Mechanisms
Friedrich Schiller University Jena
2015-2024
Jena University Hospital
2015-2024
Charité - Universitätsmedizin Berlin
2004-2022
Schiller International University
2012
Humboldt-Universität zu Berlin
2009
Franklin University
2001-2003
Freie Universität Berlin
1999-2002
Max Planck Institute of Immunobiology and Epigenetics
1996-1999
Max Planck Society
1995-1999
Max Planck Institute for Biophysical Chemistry
1997
ABSTRACT β-catenin is a multifunctional protein found in three cell compartments: the plasma membrane, cytoplasm and nucleus. The has developed elaborate ways of regulating level localization to assure its specific function each compartment. One aspect this regulation inherent structural organization itself; most protein-interacting motifs overlap so that interaction with one partner can block binding another at same time. Using recombinant proteins, we E-cadherin lymphocyte-enhancer...
Tricellulin is a tight junction protein localized in tricellular junctions (tTJs), the meeting points of three cells, but also bicellular (bTJs). To investigate its specific barrier functions bTJs and tTJs, TRIC-a was expressed low-level tricellulin-expressing MDCK II, either all TJs or only tTJs. When TJs, tricellulin increased paracellular electrical resistance decreased permeability to ions larger solutes, which are associated with enhanced ultrastructural integrity toward strand...
ABSTRACT We have produced null mutant mouse embryonic stem cells for the cell adhesion molecule E-cadherin. Such E-cadherin−/− ES are defective in aggregation; this defect can be corrected by transfection with cDNA either E-cadherin or N-cadherin driven a constitutive promoter. The presence (or absence) of regulates expression transcription factor T-brachyury, indicating that cadherins play role linking surface receptors and gene expression. Comparative analysis parental genetically altered...
Apoptotic cell death induces dramatic molecular changes in cells, becoming apparent on the structural level as membrane blebbing, condensation of cytoplasm and nucleus, loss cell-cell contacts. The activation caspases is one fundamental steps during programmed death. Here we report a detailed analysis fate Ca(2+)-dependent adhesion molecule E-cadherin apoptotic epithelial cells show that apoptosis fragments with masses 24, 29, 84 kDa are generated by two distinct proteolytic activities. In...
During postnatal development, the hair follicle (HF) shows cyclic activity with periods of relative resting, active growth (anagen), and regression. We demonstrate that similar to HF induction in embryonic skin, initiation a new phase skin requires neutralization inhibitory bone morphogenetic protein 4 (BMP4) by BMP antagonist noggin. In resting HF, BMP4 mRNA predominates over noggin epithelium mesenchyme, receptor IA is prominently expressed follicular germ. Anagen development accompanied...
Catenins are proteins associated with the cytoplasmic domain of cadherins, a family transmembrane cell adhesion molecules. The cadherin-catenin system is involved in morphogenesis during development and maintenance integrity different tissue types. Using gene trap strategy, we have isolated mouse mutation for encoding alpha-E-catenin. This form alpha-catenin appears frequently coexpressed E-cadherin epithelial obtained eliminates carboxyl-terminal third protein but nevertheless provokes...
Summary Tight junctions seal the paracellular cleft of epithelia and endothelia, form vital barriers between tissue compartments consist tight-junction-associated marvel proteins (TAMPs) claudins. The function TAMPs interaction with claudins are not understood. We therefore investigated binding occludin, tricellulin, marvelD3 their in living tight-junction-free human embryonic kidney-293 cells. In contrast to tricellulin showed no enrichment at cell–cell contacts indicating lack homophilic...
Desmosomes are intercellular junctions that confer strong cell-cell adhesion, thus conferring resistance against mechanical stress on epithelial tissues. A body of evidence indicates decreased expression desmosomal proteins is associated with poor prognosis in various cancers. As a key component plaque proteins, the functional role desmoplakin (DSP) cancer not yet elucidated. Here, we reported anti-tumorigenic activity DSP non-small cell lung (NSCLC). We found by DNA methylation was...
β-catenin, the vertebrate homolog of Drosophila Armadillo protein, has been shown to have dual cellular functions, as a component both cadherin-catenin cell adhesion complex and Wnt signaling pathway. At signaling, β-catenin becomes stabilized in cytoplasm subsequently available for interaction with transcription factors lymphocyte enhancer factor-1/T-cell factor family, resulting nuclear localization β-catenin. Although does not bind DNA directly, its carboxyl- amino-terminal regions...
β-Catenin, a member of the family Armadillo repeat proteins, plays dual role in cadherin-mediated cell adhesion and signaling by Wnt growth factors. Upon stimulation β-catenin undergoes nuclear translocation serves as transcriptional coactivator T factor DNA-binding proteins. Previously transactivation potential different portions has been demonstrated, but precise location transactivating elements not established. Also, mechanism molecular basis for functional differences between closely...
The polarized morphology of epithelial cells depends on the establishment and maintenance characteristic intercellular junctions. dramatic morphological changes observed in apoptotic were ascribed at least part to specific fragmentation components adherens junctions desmosomes. Little, however, is known about tight during apoptosis. We have found that after induction apoptosis cells, junction proteins undergo proteolytic cleavage a distinctive manner correlated with disruption transmembrane...
The exact sites, structures, and molecular mechanisms of interaction between junction organizing zona occludence protein 1 (ZO-1) the tight occludin or adherens alpha-catenin are unknown. Binding studies by surface plasmon resonance spectroscopy peptide mapping combined with comparative modeling utilizing crystal structures led for first time to a model revealing binding both same site in ZO-1. Our data support concept that ZO-1 successively associates at junction. Strong spatial evidence...
Clinical and animal studies suggest that estrogen receptors are involved in the development of myocardial hypertrophy heart failure. In this study, we investigated whether human receptor alpha (ERalpha) expression, localization, association with structural proteins was altered end stage-failing hearts. We found a 1.8-fold increase ERalpha mRNA protein end-stage dilated cardiomyopathy (DCM, n=41), as compared controls (n=25). visualized by confocal immunofluorescence microscopy localized to...