A5070820842- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Hypothalamic control of reproductive hormones
- Neuroendocrine regulation and behavior
- Chemokine receptors and signaling
- Stress Responses and Cortisol
- Neuroscience and Neuropharmacology Research
- Glaucoma and retinal disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Ocular Surface and Contact Lens
- Chemical Synthesis and Analysis
- Neurotransmitter Receptor Influence on Behavior
- Hormonal Regulation and Hypertension
- interferon and immune responses
- Corneal Surgery and Treatments
- Estrogen and related hormone effects
- Immune Response and Inflammation
- Regulation of Appetite and Obesity
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Monoclonal and Polyclonal Antibodies Research
- Pain Mechanisms and Treatments
- Pharmacological Effects and Assays
- Cancer, Stress, Anesthesia, and Immune Response
- Radiopharmaceutical Chemistry and Applications
- Retinoids in leukemia and cellular processes
Sorbonne Université
2012-2022
Inserm
2012-2022
Institut de la Vision
2013-2022
Centre National de la Recherche Scientifique
2011-2021
Délégation Paris 6
2013
Centre hospitalier national d'ophtalmologie des Quinze-Vingts
2013
Université de Versailles Saint-Quentin-en-Yvelines
2013
Hôpital Saint-Antoine
2003-2012
Centre de Recherche Saint-Antoine
1989-2009
Hypertension pulmonaire : physiopathologie et innovation thérapeutique
1993-2002
In this paper we determine the limits and accuracy of quantitative reverse transcription (RT)-PCR using a modification original protocol. The quantification mRNA with procedure requires preliminary estimation target molecule (TM) concentration, established from experiments an internal control (ICM). A definitive is then attained serial dilutions reaction. success latter step dependent on maintaining equivalent number TM ICM in purpose our study was to evaluate influence deviation between...
Abstract Accumulating evidence supports a role of chemokines and their receptors in brain function. Up to now scarce has been given the neuroanatomical distribution chemokine receptors. Although it is widely accepted that are present on glial cells, especially pathological conditions, remains unclear whether they constitutively normal rat neurons have potential express such CXCR4, G protein‐coupled receptor for stromal cell‐derived factor‐1 (SDF‐1/CXCL12) was reported possible implications...
We employed quantitative autoradiography to examine the distribution of thyrotropin-releasing hormone (TRH) receptors in rat CNS. The binding [3H]3-methyl-histidine-TRH [( 3H]MeTRH) TRH frozen brain sections was saturable, a high affinity (Kd = 5 nM), and specific for analogs. Autoradiograms [3H]MeTRH showed highest concentrations rhinencephalon, including accessory olfactory bulb, nuclei amygdala, ventral dentate gyrus subiculum hippocampus. Moderate receptor were found within thalamus...
A better understanding of the mechanisms linked to chemokine pronociceptive effects is essential for development new strategies prevent and treat chronic pain. Among chemokines, MCP-1/CCL2 involvement in neuropathic pain processing now established. However, by which exerts its are still poorly understood. In present study, we demonstrate that can alter neurotransmission healthy rats. Using immunohistochemical studies, first show CCL2 constitutively expressed primary afferent neurons their...
Dry eye disease (DED) is a multifactorial associated with ocular surface inflammation, pain, and nerve abnormalities. We studied the peripheral central neuroinflammatory responses that occur during persistent DED using molecular, cellular, behavioral, electrophysiological approaches.A mouse model of was obtained by unilateral excision extraorbital lachrymal gland (ELG) Harderian (HG) adult female C57BL/6 mice. In vivo tests were conducted at 7, 14, 21 days (d) after surgery. Tear production...
Recent studies demonstrated that the chemokine monocyte chemoattractant protein-1 (MCP-1)/CCL2 and its receptor, CCR2, play important roles in various brain diseases. In this study, using quantitative autoradiography, we studied pharmacological properties of [125I]MCP-1/CCL2 binding rat clearly showed distribution CCR2 receptors cerebral cortex, nucleus accumbens, striatum, amygdala, thalamus, hypothalamus, hippocampus, substantia nigra, mammillary bodies raphe nuclei. Moreover, double...
The monocyte chemoattractant protein-1 (MCP-1/CCL2) and its receptor CCR2 are key modulators of immune functions. In the nervous system, MCP-1/CCL2 is implicated in neuroinflammatory pathologies. However, cerebral functions under normal conditions still unclear. this study, using reverse transcriptase-polymerase chain reaction (RT-PCR) specific rat MCP-1 enzyme-linked immunosorbent assay (ELISA) approaches, we observed that mRNA protein were expressed different punched regions central...
Abstract The stromal cell‐derived factor‐1 (SDF‐1)/CXCL12 and its receptor CXCR4 are key modulators of immune functions. In the nervous system, SDF‐1/CXCL12 is crucial for neuronal guidance in developing brain, intercellular communication neuropathogenesis acquired immunodeficiency syndrome. However, cerebral functions adult brain poorly understood. understanding role needs a detailed neuroanatomical mapping SDF‐1/CXCL12. By dual immunohistochemistry we demonstrate that this chemokine...
Abstract In the CNS, immune‐like competent cells (microglia and astrocytes) were first described as potential sites of chemokine synthesis, but more recent evidence has indicated that neurones might also express chemokines their receptors. The aim present work was to investigate further, both in vivo vitro, CC Chemokine Family Receptor 2 (CCR2) expression functionality rat spinal cord neurones. First, we demonstrated by RT–PCR western blot analysis CCR2 mRNA protein extracts. Furthermore,...
Abstract Chemokines and their receptors are well described in the immune system, where they promote cell migration activation. In central nervous chemokine has been implicated neuroinflammatory processes. However, an increasing number of evidence suggests that have regulatory functions normal could participate communication. this work, using a semiquantitative immunohistochemistry approach, we provide first neuroanatomical mapping constitutive neuronal CCR2 localization. Neuronal expression...
In the rat brain, destruction of dopaminergic cell groups by injections 6-hydroxydopamine into ventral mesencephalic tegmentum results in large decreases number neurotensin binding sites mesencephalon and striatum. contrast, these lesions produce an increase 125I-labeled lateral part prefrontal cortex despite a decrease cortical dopamine levels. Increases this area as well entorhinal cortex, nucleus accumbens, central striatum were also obtained after chronic blockade neurotransmission...