A5078504380- Nerve injury and regeneration
- RNA Interference and Gene Delivery
- Autism Spectrum Disorder Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Alzheimer's disease research and treatments
- Tryptophan and brain disorders
- Parkinson's Disease Mechanisms and Treatments
- Nuclear Receptors and Signaling
- Ultrasound and Hyperthermia Applications
- Neuropeptides and Animal Physiology
- Vagus Nerve Stimulation Research
- Fibroblast Growth Factor Research
- Virus-based gene therapy research
- Trace Elements in Health
- Peroxisome Proliferator-Activated Receptors
- Obsessive-Compulsive Spectrum Disorders
- Neurogenesis and neuroplasticity mechanisms
- Inflammasome and immune disorders
- Signaling Pathways in Disease
- Cerebral Venous Sinus Thrombosis
- Neuroscience and Neuropharmacology Research
- RNA Research and Splicing
Instituto Politécnico Nacional
2015-2023
Center for Research and Advanced Studies of the National Polytechnic Institute
2010-2023
Colciencias
2012
The neurotrophin Brain-Derived Neurotrophic Factor (BDNF) influences nigral dopaminergic neurons via autocrine and paracrine mechanisms. reduction of BDNF expression in Parkinson's disease substantia nigra (SN) might contribute to the death because inhibiting SN causes parkinsonism rat. This study aimed demonstrate that increasing rats with one week 6-hydroxydopamine lesion recovers from parkinsonism. plasmids phDAT-BDNF-flag phDAT-EGFP, coding for enhanced green fluorescent protein, were...
Models of Parkinson’s disease with neurotoxins have shown that microglial activation does not evoke a typical inflammatory response in the substantia nigra, questioning whether neuroinflammation leads to neurodegeneration. To address this issue, archetypal stimulus, lipopolysaccharide (LPS), was injected into rat nigra. LPS induced fever, sickness behavior, and (OX42 immunoreactivity), followed by astrocyte leukocyte infiltration (GFAP CD45 immunoreactivities). During acute phase...
Whether neuroinflammation leads to dopaminergic nigrostriatal system neurodegeneration is controversial. We addressed this issue by inducing acute in the substantia nigra (SN) with a single local administration (5 µg/2 µL saline solution) of lipopolysaccharide (LPS). Neuroinflammatory variables were assessed from 48 h 30 days after injury immunostaining for activated microglia (Iba-1 +), neurotoxic A1 astrocytes (C3 + and GFAP active caspase-1. also evaluated NLRP3 activation Il-1β levels...
. Sucrose consumption activates reward mechanisms in the brain by releasing dopamine. The mechanism has two components that can be dissociated: ‘wanting,’ related to consumption, and ‘liking,’ associated with preference. aim of this study was demonstrate damage dorsal striatum affects both ‘wanting’ ‘liking.’ Sixteen male Wistar rats weighing 250 ± 5 g were used, divided into groups: sham lesioned. In second group, ipsilaterally lesioned administering 6-OHDA-HCl (6.7 μg/μL) at a flow rate...
The structural effect of neurturin (NRTN) on the nigrostriatal dopaminergic system in animals remains unknown, although NRTN has been shown to be effective Parkinson's disease animal models. Herein, we aimed demonstrate that overexpression neurons stimulates both neurite outgrowths pathway and striatal dendritic spines aging rats with chronic 6-hydroxydopamine (6-OHDA) lesion. At week 12 after lesion, pTracer-mNRTN-His or pGreenLantern-1 plasmids were intranigrally transfected using...
The spreading and accumulation of α-synuclein dopaminergic neurodegeneration, two hallmarks Parkinson's disease (PD), have been faithfully reproduced in rodent brains by chronic, oral administration β-sitosterol β-D-glucoside (BSSG). We investigated whether a single injection BSSG (6 μg BSSG/μL DMSO) the left substantia nigra Wistar rats causes same effects. Mock DMSO injections untreated formed control groups. performed immunostainings against pathological α-synuclein, marker tyrosine...
Chronic consumption of β-sitosterol-β-D-glucoside (BSSG), a neurotoxin contained in cycad seeds, leads to Parkinson’s disease humans and rodents. Here, we explored whether single intranigral administration BSSG triggers neuroinflammation neurotoxic A1 reactive astrocytes besides dopaminergic neurodegeneration. We injected 6 μg BSSG/1 μL DMSO or vehicle into the left substantia nigra immunostained with antibodies against tyrosine hydroxylase (TH) together markers microglia (OX42), (GFAP,...
JOURNAL/nrgr/04.03/01300535-202409000-00039/figure1/v/2024-01-30T062302Z/r/image-tiff Parkinsonism by unilateral, intranigral β-sitosterol β-D-glucoside administration in rats is distinguished that the α-synuclein insult begins unilaterally but spreads bilaterally and increases severity over time, thus replicating several clinical features of Parkinson's disease, a typical α-synucleinopathy. As Nurr1 represses α-synuclein, we evaluated whether unilateral transfected rNurr1-V5 transgene via...
Neurotensin (NTS)-polyplex is a multicomponent nonviral vector that enables gene delivery via internalization of the neurotensin type 1 receptor (NTSR1) to dopaminergic neurons and cancer cells. An approach improving its therapeutic safety replacing viral karyophilic component (peptide KPSV40; MAPTKRKGSCPGAAPNKPK), which performs nuclear import activity, by shorter synthetic peptide (KPRa; KMAPKKRK). We explored this issue mechanism plasmid DNA translocation through expression green...
Overexpression of neurotrophic factors in nigral dopamine neurons is a promising approach to reverse neurodegeneration the nigrostriatal system, hallmark Parkinson’s disease. The human cerebral factor (hCDNF) has recently emerged as strong candidate for disease therapy. This study shows that hCDNF expression using neurotensin-polyplex nanoparticle system reverses 6-hydroxydopamine-induced morphological, biochemical, and behavioral alterations. Three independent electron microscopy techniques...
Abstract Neurotensin-polyplex nanoparticles provide efficient gene transfection of nigral dopaminergic neurons when intracerebrally injected in preclinical trials Parkinson’s disease because they do not cross the blood–brain barrier (BBB). Therefore, this study aimed to open BBB with focused ultrasound (FUS) on substantia nigra attain systemic and intranasal transfections evaluate its detrimental effect rats. Systemically Evans Blue showed that a two-pulse FUS opened BBB. Accordingly, 35 μL...