A5046682974- Mesenchymal stem cell research
- Diabetic Foot Ulcer Assessment and Management
- Tissue Engineering and Regenerative Medicine
- RNA Interference and Gene Delivery
- Wound Healing and Treatments
- Angiogenesis and VEGF in Cancer
- Antimicrobial Peptides and Activities
- Virus-based gene therapy research
- Electrospun Nanofibers in Biomedical Applications
- Peripheral Artery Disease Management
- Periodontal Regeneration and Treatments
- Retinal Diseases and Treatments
- Cancer Cells and Metastasis
- Corneal Surgery and Treatments
- Oral health in cancer treatment
- Transgenic Plants and Applications
- Monoclonal and Polyclonal Antibodies Research
- Porphyrin Metabolism and Disorders
- Nanoplatforms for cancer theranostics
- Intraocular Surgery and Lenses
- Hydrogels: synthesis, properties, applications
- Nanoparticle-Based Drug Delivery
- Retinal and Macular Surgery
- Advanced Drug Delivery Systems
- Folate and B Vitamins Research
Universidad Autónoma de Bucaramanga
2016-2024
Foscal Hospital
2016-2022
Universidad del Desarrollo
2012-2016
Clínica Alemana
2012-2016
Center for Research and Advanced Studies of the National Polytechnic Institute
2006-2009
Universidad Nacional Autónoma de México
2006
Mesenchymal stromal cells (MSCs) have shown to be a promising tool in cell therapies treat different conditions. Several pre-clinical and clinical studies proved that the transplantation of MSCs improves wound healing. Here, we compare beneficial effects mouse bone marrow-derived allogeneic (allo-mBM-MSCs) their acelullar derivatives (allo-acd-mMSCs) on skin healing Non-Obese Diabetic (NOD) mice. One dose allo-mBM-MSCs (1×106 cells) or one allo-acd-mMSCs (1X) were intradermally injected...
The final product of adipogenesis is a functional adipocyte. This mature cell acquires the necessary machinery for lipid metabolism, loses its proliferation potential, increases insulin sensitivity, and secretes adipokines. Multipotent mesechymal stromal cells have been recognized as source adipocytes both in vivo vitro. vitro adipogenic differentiation human MSC (hMSC) has induced up to now by using complex stimulus which includes dexamethasone, 3-isobutyl-1-methylxanthine, indomethacin, (a...
Preclinical and clinical studies have shown that a therapeutic effect results from mesenchymal stromal cells (MSCs) transplant. No systematic information is currently available regarding whether donor age modifies MSC regenerative potential on cutaneous wound healing. Here, we evaluate influences this potential. Two different doses of bone marrow MSCs (BM‐MSCs) young, adult, or old mouse donors two their acellular derivatives (acd‐MSCs) were intradermally injected around wounds in the...
Chronic limb-threatening ischemia (CLTI) represents the final stage of peripheral arterial disease. Approximately one-third patients with CLTI are not eligible for conventional surgical treatments. Furthermore, advanced prone to amputation and death. Thus, an effective therapeutic strategy is urgently needed. In this context, autologous bone marrow mononuclear cell (auto-BM-MNC) allogeneic mesenchymal stem cells represent a promising approach treating CLTI. study, we compared safety...
Several short linear peptides derived from cyclic bovine lactoferricin were synthesized and tested for their cytotoxic effect against the oral cavity squamous-cell carcinoma (OSCC) cell lines CAL27 SCC15. As a control, an immortalized nontumorigenic line, Het-1A, was used. Linear based on RRWQWR core sequence showed moderate specificity towards tumorigenic cells. A tetrameric peptide, LfcinB(20–25) 4 , containing motif, exhibited greater activity (>90%) in both OSCC compared to peptide or...
Background Vitamin B12 is indispensable for proper brain functioning and cytosolic synthesis of S-adenosylmethionine. Whether its deficiency produces effects on viability apoptosis neurons remains unknown. There a particular interest in investigating these Parkinson disease where Levodopa treatment known to increase the consumption To cause deprivation vitamin B12, we have recently developed cell model that decreased S-adenosylmethionine by anchoring transcobalamin (TCII) reticulum through...
The human breast adenocarcinoma cell line MDA-MB-231 has the triple-negative cancer (TNBC) phenotype, which is an aggressive subtype with no specific treatment. cells express neurotensin receptor type 1 (NTSR1), makes these attractive target of therapeutic genes that are delivered by (NTS)-polyplex nanocarrier via bloodstream. We addressed relevance this strategy for TNBC treatment using NTS-polyplex nanoparticles harboring herpes simplex virus thymidine kinase (HSVtk) suicide gene and its...
Multipotent stromal cells (MSCs) are envisioned as a powerful therapeutic tool. As they home into tumors, secrete trophic and vasculogenic factors, suppress immune response their role in carcinogenesis is matter of controversy. Worldwide oral squamous cell carcinoma (OSCC) the fifth most common epithelial cancer. Our aim was to determine whether MSC administration at precancerous stage modifies natural progression OSCC. OSCC induced Syrian hamsters by topical application DMBA buccal pouch....
Oral squamous cell carcinoma is the fifth most common epithelial cancer in world, and its current clinical treatment has both low efficiency poor selectivity. Cationic amphipathic peptides have been proposed as new drugs for of different types cancer. The main goal present work was to determine potential LfcinB(20-25)4, a tetrameric peptide based on core sequence RRWQWR bovine lactoferricin LfcinB(20-25), OSCC. In brief, OSCC induced buccal pouch hamsters by applying...
Summary Diabetic foot ulcer morbidity and mortality are dramatically increasing worldwide, reinforcing the urgency to propose more effective interventions treat such a devastating condition. Previously, using diabetic mouse model, we demonstrated that administration of bone marrow mesenchymal stem cells derivatives is than use alone. Here, used aforementioned treatments on three patients with grade 2 ulcers assessed their beneficial effects, relative conventional approach. In present study,...
Background Oleosin is a plant protein localized to lipid droplets and endoplasmic reticulum of cells. Our idea was use it target functional secretory proteins interest the cytosolic side mammalian cells, through expressing oleosin-containing chimeras. We have designed this approach create cellular models deficient in vitamin B12 (cobalamin) because known problematics associated obtainment effective cell models. This achieved by overexpression transcobalamin inside cells anchoring oleosin....