A5011805055- Ion channel regulation and function
- Cardiac electrophysiology and arrhythmias
- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Hormonal Regulation and Hypertension
- Ion Transport and Channel Regulation
- Ion Channels and Receptors
- Neuroscience and Neural Engineering
- Cardiovascular Function and Risk Factors
- Metabolism, Diabetes, and Cancer
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Calcium signaling and nucleotide metabolism
- Cardiomyopathy and Myosin Studies
- Cardiac Fibrosis and Remodeling
- Molecular Biology Techniques and Applications
- Nitric Oxide and Endothelin Effects
- Protein Kinase Regulation and GTPase Signaling
- Environmental Education and Sustainability
- Neurological Disorders and Treatments
- Sodium Intake and Health
- Signaling Pathways in Disease
- Pain Mechanisms and Treatments
- 14-3-3 protein interactions
- Animal testing and alternatives
- Mast cells and histamine
Universidad De Santander
2022-2023
Instituto Politécnico Nacional
2013-2022
Center for Research and Advanced Studies of the National Polytechnic Institute
2013-2022
Inserm
2009-2018
Université Paris-Saclay
2018
Université Paris-Sud
2018
University of Michigan–Ann Arbor
2017
Université de Montpellier
2011-2013
University of Wisconsin–Madison
2003-2009
Cardiff University
2009
Cardiac ryanodine receptor (RyR2) mutations are associated with autosomal dominant catecholaminergic polymorphic ventricular tachycardia, suggesting that alterations in Ca(2+) handling underlie this disease. Here we analyze the underlying release defect leads to arrhythmia cardiomyocytes isolated from heterozygous knock-in mice carrying RyR2(R4496C) mutation. RyR2(R4496C-/-) littermates (wild type) were used as controls. [Ca(2+)](i) transients obtained by field stimulation fluo-3-loaded and...
The mineralocorticoid pathway is involved in cardiac arrhythmias associated with heart failure through mechanisms that are incompletely understood. Defective regulation of the ryanodine receptor (RyR) an important cause initiation arrhythmias. Here, we examined whether aldosterone might modulate RyR function.Using whole-cell patch clamp method, observed increase occurrence delayed afterdepolarizations during action potential recordings isolated adult rat ventricular myocytes exposed for 48...
Activation of Ca2+ release channels/ryanodine receptors (RyR) by the inward current (ICa) gives rise to Ca2+-induced (CICR), amplifying signaling mechanism that triggers contraction heart. CICR, in theory, is a high-gain, self-regenerating process, but an unidentified stabilizes it vivo. We reported previously (Lokuta, A. J., Meyers, M. B., Sander, P. R., Fishman, G. I., and Valdivia, H. (1997) J. Biol. Chem. 272, 25333-25338) sorcin, 22-kDa Ca2+-binding protein, binds cardiac RyRs with high...
TRPV4 (transient receptor potential vanilloid 4) is a cation channel activated by hypotonicity, moderate heat, or shear stress. We describe the expression of during differentiation corneal epithelial cell model, RCE1(5T5) cells. late feature that concentrated in apical membrane outmost layer stratified epithelia. Ca2+ imaging experiments showed activation with GSK1016790A produced an influx calcium was blunted specific blocker RN-1734. analyzed involvement measuring development...
Klotho is a membrane-bound or soluble protein, originally identified as an age-suppressing factor and regulator of mineral metabolism. deficiency associated with the development renal disease, but its role in cardiac function context uraemic cardiomyopathy unknown.We explored effects on Ca2+ cycling. We analysed handling adult cardiomyocytes from Klotho-deficient (kl/kl) mice murine model 5/6 nephrectomy (Nfx). also studied effect exogenous supplementation, by chronic recombinant treatment,...
Diabetes is a major risk factor for stroke. However, the molecular mechanisms involved in cerebral artery dysfunction found diabetic patients are not completely elucidated. In smooth muscle cells (CASMCs), spontaneous and local increases of intracellular Ca2+ due to opening ryanodine receptors (Ca2+ sparks) activate large conductance Ca2+-activated K+ (BK) channels that generate transient outward currents (STOCs). STOCs have key participation control vascular myogenic tone blood pressure....
The penta-EF hand protein sorcin participates in the modulation of Ca2+-induced calcium-release heart through interaction with several Ca2+ channels such as ryanodine receptor. modulating activity is impaired recently described natural F112L mutant. F112 residue located at end D helix next to Asp113, one calcium ligands EF3 endowed highest affinity for metal. F112L-sorcin X-ray crystal structure 2.5 Å resolution displays marked alterations hand, where hydrogen bonding network established by...
Abnormalities in cardiomyocyte Ca2+ handling contribute to impaired contractile function heart failure (HF). Experiments on single ryanodine receptors (RyRs) incorporated into lipid bilayers have indicated that RyRs from failing hearts are more active than those healthy hearts. Here, we analyzed spontaneous sparks (brief, localized increased [Ca2+]i) evaluate RyR cluster activity situ a mouse post-myocardial infarction (PMI) model of HF. The cardiac ejection fraction PMI mice was reduced...
Inflammation is a significant contributor to cardiovascular disease and its complications; however, whether the myocardial inflammatory response harmonized after cardiac injury remains be determined. Some receptors of innate immune system, including nucleotide-binding oligomerization domain-like (NLRs), play key roles in host damage. Nucleotide-binding domain containing 1 (NOD1), member NLR family, expressed heart, but functional role has not been elucidated. We determine selective NOD1...
Metabolic syndrome (MetS) has become a global epidemic. MetS is serious health problem because of its related cardiovascular complications, which include hypertension and delayed heart rate recovery after exercise. The molecular bases cardiac dysfunction in are still under scrutiny may be to anomalies the activity expression key proteins involved excitation-contraction coupling (ECC). Ca2+ channel/ryanodine receptor (RyR2) participates releasing from internal stores plays role modulation...
Metabolic syndrome (MetS) is a cluster of cardiometabolic risk factors, with insulin resistance as critical component for its development. Insulin signaling in the heart leads to Akt (also known PKB) activation, serine/threonine protein kinase, which regulates cardiac glucose metabolism and growth. Cardiac metabolic inflexibility, characterized by impaired insulin-induced uptake oxidation, has been reported an early consistent change different models MetS diabetes; however, evaluation...
The MR (mineralocorticoid receptor) antagonists belong to the current therapeutic armamentarium for management of cardiovascular diseases, but mechanisms conferring their beneficial effects are poorly understood. Part is because regulation L-type Cav1.2 Ca2+ channel expression, which generated by tissue-specific alternative promoters as a long cardiac or short vascular N-terminal transcripts.To analyze molecular aldosterone, through MR, modulates expression and function in manner.In primary...
Spontaneous, local Ca(2+) release events or sparks by ryanodine receptors (RyRs) are important determinants of vascular tone and arteriolar resistance, but the mechanisms that modulate their properties in smooth muscle poorly understood. Sorcin, a Ca(2+)-binding protein associates with cardiac RyRs quickly stops heart, provides potential mechanism to muscle, little is known about functional role sorcin this tissue. In work, we characterized expression intracellular location aorta cerebral...
Ca2+ mediates the functional coupling between L-type channel (LTCC) and sarcoplasmic reticulum (SR) release (ryanodine receptor, RyR), participating in key pathophysiological processes. This crosstalk manifests as orthograde Ca2+-induced Ca2+-release (CICR) mechanism triggered by influx, but also retrograde Ca2+-dependent inactivation (CDI) of LTCC, which depends on both permeating through LTCC itself SR RyR. latter effect has been suggested to rely local rather than global signaling, might...
Activation of Ca2+ release channels/ryanodine receptors (RyR) by the inward current (I(Ca)) gives rise to Ca(2+)-induced (CICR), amplifying signaling mechanism that triggers contraction heart. CICR, in theory, is a high-gain, self-regenerating process, but an unidentified stabilizes it vivo. Sorcin, 21.6 kDa Ca(2+)-binding protein, binds cardiac RyRs with high affinity and completely inhibits channel activity. Sorcin significantly both spontaneous activity quiescent cells (visualized as...
Abstract TRPV4 is a nonselective cationic channel responsive to several physical and chemical stimuli. Defects in function result human diseases, such as skeletal dysplasias, arthropathies, peripheral neuropathies. Nonetheless, little known about the role of other cellular functions, nuclear Ca 2+ homeostasis or ‐regulated transcription. Here, we confirmed presence full‐length nuclei nonpolarized Madin‐Darby canine kidney cells. Confocal imaging showed that activation increases cytoplasmic...