A5083020535- Parathyroid Disorders and Treatments
- Blood Pressure and Hypertension Studies
- Hormonal Regulation and Hypertension
- Cardiovascular Function and Risk Factors
- Protease and Inhibitor Mechanisms
- Dialysis and Renal Disease Management
- Advanced Proteomics Techniques and Applications
- Chronic Kidney Disease and Diabetes
- Magnesium in Health and Disease
- Cardiac Imaging and Diagnostics
- Pulmonary Hypertension Research and Treatments
- Cardiac electrophysiology and arrhythmias
- Lipoproteins and Cardiovascular Health
- Muscle and Compartmental Disorders
- Acute Kidney Injury Research
- Cardiovascular Health and Disease Prevention
- Health, Environment, Cognitive Aging
- Cardiovascular Health and Risk Factors
- Metabolomics and Mass Spectrometry Studies
- Connective tissue disorders research
- Neurological and metabolic disorders
- Renin-Angiotensin System Studies
- Sodium Intake and Health
- Blood Coagulation and Thrombosis Mechanisms
- Diabetes, Cardiovascular Risks, and Lipoproteins
Hospital Universitario 12 De Octubre
2017-2025
Research Institute Hospital 12 de Octubre
2017-2025
Magna Graecia University
2020
Multidisciplinary Digital Publishing Institute (Switzerland)
2020
Hospital Universitario Fundación Jiménez Díaz
2018
Abstract Ischaemic heart disease (IHD) remains a major cause of death and morbidity. Klotho is well‐known anti‐ageing factor with relevant cardioprotective actions, at least when renal dysfunction present, but its actions are much less known function preserved. This study investigated as biomarker potential novel treatment IHD‐associated complications after myocardial infarction (MI) under preserved function. Association between circulating levels cardiac injury was in patients ST‐elevation...
Renal ischemia and reperfusion injury (IRI) is the main cause of acute kidney (AKI). AKI induces development cardiac hypertrophy (CH) during cardiorenal syndrome (CRS), cardiomyocyte calcium mishandling though systemic inflammation after 8 days renal IRI. Klotho has recently been described as an anti-inflammatory component. Given this, treatment could prevent or attenuate inflammation, thereby also preventing electrical outcomes incurred by CRS. The aim this study was to investigate...
Cardiac dysfunction and arrhythmia are common onerous cardiovascular events in end-stage renal disease (ESRD) patients, especially those on dialysis. Fibroblast growth factor (FGF)-23 is a phosphate-regulating hormone whose levels dramatically increase as function declines. Beyond its role phosphorus homeostasis, FGF-23 may elicit direct effect the heart. Whether modulates ventricular cardiac rhythm unknown, prompting us to study excitation-contraction (EC) coupling.We examined vitro actions...
Objective To compare the risk of cardiovascular disease and occurrence events in mid-long term after delivery, between women with without a history early-onset preeclampsia.
Klotho is a membrane-bound or soluble protein, originally identified as an age-suppressing factor and regulator of mineral metabolism. deficiency associated with the development renal disease, but its role in cardiac function context uraemic cardiomyopathy unknown.We explored effects on Ca2+ cycling. We analysed handling adult cardiomyocytes from Klotho-deficient (kl/kl) mice murine model 5/6 nephrectomy (Nfx). also studied effect exogenous supplementation, by chronic recombinant treatment,...
Abstract Background Chronic kidney disease (CKD) is associated with increased propensity for arrhythmias. In this context, ventricular repolarization alterations have been shown to predispose fatal arrhythmias and sudden cardiac death. Between mineral bone disturbances in CKD patients, fibroblast growth factor (FGF) 23 decreased Klotho are emerging as important effectors of cardiovascular disease. However, the relationship between imbalanced FGF23-Klotho axis development remains unknown....
Hemodialysis patients experience high oxidative stress because of systemic inflammation and depletion antioxidants. Little is known about the global status during dialysis or whether it linked to type dialysis. We investigated before (pre-) after (post-) one session in subjected high-flux (HFD) on-line hemodiafiltration (OL-HDF). analyzed carbonyls, oxidized LDL (oxLDL), 8-hydroxy-2'-deoxyguanosine, xanthine oxidase (XOD) activity as markers, total antioxidant capacity (TAC), catalase,...
Acute renal failure (ARF) following ischemia-reperfusion (I/R) injury is considered a relevant risk factor for cardiac damage, but the underlying mechanisms, particularly those triggered at cardiomyocyte level, are unknown.We examined intracellular Ca2+ dynamics in adult ventricular cardiomyocytes isolated from C57BL/6 mice 7 or 15 days unilateral I/R.After of I/R, cell contraction was significantly lower compared to sham-treated mice. It accompanied by significant decrease both systolic...
Calcific aortic valve disease (CAVD) and coronary artery (CAD) are related cardiovascular diseases in which common mechanisms lead to tissue calcification. Oxidative stress plays a key role these there is also evidence that the redox state of serum albumin exerts significant influence on conditions. To further explore this issue, we used multimarker scores (OxyScore AntioxyScore) assess global oxidative status patients with CAVD, without CAD, evaluating their plasma thiol levels. In...
Aims: Current cardiovascular (CV) risk prediction algorithms are able to quantify the individual of CV disease. However, in young adults is underestimated due high dependency age biomarker-based algorithms. Because oxidative stress associated with disease, we sought examine stratification based on approach discovery new markers for early detection pathology. Results: Young were stratified into (i) healthy controls, (ii) subjects factors, and (iii) patients a reported event. Plasma samples...
The protocol describes a novel, rapid, and no-wash one-step immunoassay for highly sensitive direct detection of the complexes between matrix metalloproteinases (MMPs) their tissue inhibitor (TIMPs) based on AlphaLISA® technology. We describe two procedures: (i) one approach is used to analyze MMP-9-TIMP-1 interactions using recombinant human MMP-9 with its corresponding TIMP-1 (ii) second native or endogenous protein in samples plasma. Evaluating this avoids use indirect calculations...
Las enzimas metaloproteinasas de matriz (MMP) están involucradas en el remodelado tisular deletéreo asociado al daño órganos diana la enfermedad renal. El objetivo este estudio fue explorar asociación entre caída función renal y actividad sistémica metaloproteinasa inflamatoria MMP-9 paciente hipertenso con crónica (ERC) leve-moderada. Se analizaron los niveles plasmáticos activa, total, su inhibidor (TIMP-1), cociente MMP-9/TIMP-1 interacción TIMP-1 37 pacientes hipertensos distribuidos...
Background. Myeloid-derived suppressor cells (MDSCs) increase in patients with cancer and are associated poor prognosis; however, their role transplantation is not yet understood. Here we aimed to study the MDSC effects on evolution of kidney transplant recipients (KTRs). Methods. A cohort 229 KTRs was prospectively analyzed. Two myeloid subsets. CD11b + CD33 CD14 CD15 - HLA-DR –/low (monocytic [M-MDSC]) – (monocytes), were defined by flow cytometry. The suppressive capacity tested...
Abstract The evaluation of cardiovascular (CV) risk is based on equations derived from epidemiological data in individuals beyond the limits middle age such as Framingham and SCORE assessments. Lifetime Risk calculator (QRisk ® ), estimates CV throughout a subjects’ lifetime, allowing those. A more aggressive earlier intervention to be identified offered protection consequences renal disease. search for molecular profiles young people that allow correct stratification would great interest...
Calcific aortic stenosis (CAS) and type 2 diabetes mellitus (T2DM) are related often concomitant pathologies, accompanied by common comorbidities such as hypertension or dyslipidemia. Oxidative stress is one of the mechanisms that trigger CAS, it can drive vascular complications in T2DM. Metformin inhibit oxidative stress, yet its effects have not been studied context CAS. Here, we assessed global status plasma from patients with both alone T2DM (and under treatment metformin), using...
Oxidized low-density lipoprotein (oxLDL) is associated with cardiac damage and causes injury to multiple cell types. We aimed investigate the role of oxLDL in ventricular stress. first examined association between circulating N-terminal pro-brain natriuretic peptide (NT-proBNP), a marker myocardial stress, young subjects (30–50 years) or without stable coronary artery disease (SCAD). NT-proBNP were significantly higher at high cardiovascular risk (CVR) than low CVR independently traditional...
Matrix metalloproteinases (MMPs) are involved in deleterious tissue remodelling associated with target organ damage renal disease. The aim of this study was to the association between dysfunction and activity inflammatory metalloproteinase MMP-9 hypertensive patients mild-moderate chronic kidney disease (CKD). Plasmatic active MMP-9, total inhibitor (TIMP-1), MMP-9/TIMP-1 ratio MMP-9-TIMP-1 interaction were analysed 37 distributed by estimated glomerular filtration rate (eGFR) 3 groups: >90,...
Renal replacement therapy (RRT) is complicated by a chronic state of inflammation and high mortality risk. However, different RRT modalities can have selective impact on markers oxidative stress. We evaluated the levels active matrix metalloproteinase (MMP)-9 in patients undergoing two types dialysis (high-flux (HFD) on-line hemodiafiltration (OL-HDF)) kidney transplantation (KT) recipients. Active MMP-9 was measured zymography ELISA before (pre-) after (post-) one session, at baseline...