A5023267538- Cardiac electrophysiology and arrhythmias
- Neuroscience and Neural Engineering
- Ion channel regulation and function
- Phosphodiesterase function and regulation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Receptor Mechanisms and Signaling
- Heart Failure Treatment and Management
- Sperm and Testicular Function
- Signaling Pathways in Disease
- Cardiovascular Function and Risk Factors
- Metabolism, Diabetes, and Cancer
- Cardiac Fibrosis and Remodeling
- Ion Channels and Receptors
- Adipose Tissue and Metabolism
- Cardiac pacing and defibrillation studies
- Heart Rate Variability and Autonomic Control
- Cardiomyopathy and Myosin Studies
- Digestive system and related health
- Ion Transport and Channel Regulation
- Health, Medicine and Society
- Hormonal Regulation and Hypertension
- Reproductive Biology and Fertility
- Wireless Sensor Networks for Data Analysis
- Reproductive System and Pregnancy
- Sensor Technology and Measurement Systems
Laboratory Signaling and Cardiovascular Pathophysiology
2018-2024
Inserm
2018-2024
Université Paris-Saclay
2018-2024
Hospital Universitari Joan XXIII de Tarragona
2024
Instituto de Investigación Sanitaria La Fe
2024
New York University
2024
Université Paris-Sud
2018-2020
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2020
Universidade do Porto
2020
Institut Galien Paris-Saclay
2019
The cyclic AMP (adenosine monophosphate; cAMP)-hydrolyzing protein PDE4B (phosphodiesterase 4B) is a key negative regulator of cardiac β-adrenergic receptor stimulation. deficiency leads to abnormal Ca2+ handling and decreased in pressure overload hypertrophy, suggesting that increasing the heart beneficial failure.We measured expression human tissues developed 2 transgenic mouse lines with cardiomyocyte-specific overexpression an adeno-associated virus serotype 9 encoding PDE4B. Myocardial...
Background: Orai1 is a critical ion channel subunit, best recognized as mediator of store-operated Ca 2+ entry (SOCE) in nonexcitable cells. SOCE has recently emerged key contributor cardiac hypertrophy and heart failure but the relevance still unclear. Methods: To test role these channels pathophysiology, transgenic mouse was generated with cardiomyocyte-specific expression an pore-disruptive R91W mutant (C-dnO1). Synthetic chemistry screening strategies were used to develop...
Dysfunction of the sinoatrial node (SAN), natural heart pacemaker, is common in failure (HF) patients. SAN spontaneous activity relies on various ion currents plasma membrane (voltage clock), but intracellular Ca2+ ([Ca2+]i) release via ryanodine receptor 2 (RYR2; clock) plays an important synergetic role. Whereas remodeling voltage-clock components has been revealed HF, less known about possible alterations to clock. Here, we analyzed [Ca2+]i handling from a mouse HF model after transverse...
The MR (mineralocorticoid receptor) antagonists belong to the current therapeutic armamentarium for management of cardiovascular diseases, but mechanisms conferring their beneficial effects are poorly understood. Part is because regulation L-type Cav1.2 Ca2+ channel expression, which generated by tissue-specific alternative promoters as a long cardiac or short vascular N-terminal transcripts.To analyze molecular aldosterone, through MR, modulates expression and function in manner.In primary...
Abstract We conducted a genome-wide association study in large population of infertile men due to unexplained spermatogenic failure (SPGF). More than seven million genetic variants were analysed 1,274 SPGF cases and 1,951 unaffected controls from two independent European cohorts. Two genomic regions associated with the most severe histological pattern SPGF, defined by Sertoli cell-only (SCO) phenotype, namely MHC class II gene HLA-DRB1 (rs1136759, P = 1.32E-08, OR 1.80) an upstream locus...
FKBP12.6, a binding protein to the immunosuppressant FK506, which also binds ryanodine receptor (RyR2) in heart, has been proposed regulate RyR2 function and have antiarrhythmic properties. However, level of FKBP12.6 expression normal hearts remains elusive some controversies still persist regarding its effects, both basal conditions during β-adrenergic stimulation. We quantified left ventricles (LV) WT (wild-type) mice two novel transgenic models expressing distinct levels using custom-made...
Regulation of vascular tone by 3',5'-cyclic adenosine monophosphate (cAMP) involves many effectors including the large conductance, Ca2+-activated, K+ (BKCa) channels. In arteries, cAMP is mainly hydrolyzed type 3 and 4 phosphodiesterases (PDE3, PDE4). Here, we examined specific contribution BKCa channels to regulation these PDEs in rat coronary how this altered heart failure (HF).Concomitant application PDE3 (cilostamide) PDE4 (Ro-20-1724) inhibitors increased unitary channel activity...
Abstract Background The AMP-activated protein kinase (AMPK) is a major regulator of cellular energetics which plays key role in acute metabolic response and long-term adaptation to stress. Recent works have also suggested non-metabolic effects. Methods To decipher AMPK roles the heart, we generated cardio-specific inducible model gene deletion main cardiac catalytic subunit ( Ampkα2 ) mice. This allowed us avoid eventual impact AMPK-KO peripheral organs. Results Cardio-specific deficiency...
We reported previously that the thromboxane A 2 (TxA ) mimetic U-46619 stimulates cardiac vagal afferent nerves, eliciting a reflex decrease in heart rate (HR) and arterial blood pressure (ABP). The present experiments were designed to test hypothesis TxA evokes these changes via release of serotonin [5-hydroxytryptamine (5-HT)] activation 5-HT 3 receptor. Injections antagonist tropisetron (1 mg 3-tropanyl-indole-3-carboxylate or ICS-205-930) attenuated decreases HR ABP induced by left...
Suppression of the testicular T production by effect CSF has been previously reported. Changes in content intermediates and precursors biosynthesis were determined rats fed during 28 days with diets containing 25, 35 50% compared a defated cottonseed flour diet group control Purina-chow group. Also described modifications six wk post period. The results showed diminution pregnenolone progesterone to third half values (21.5 19.3 pg/mg protein respectively). Other compounds Δ5 pathway, 17...
BACKGROUND: The heart expresses 2 main subtypes of cAMP-dependent protein kinase (PKA; type I and II) that differ in their regulatory subunits, RIα RIIα. Embryonic lethality knockout mice limits the current understanding PKA function myocardium. objective this study was to test role adult contractility pathological remodeling. METHODS: We measured subunit expression human developed a conditional mouse model with cardiomyocyte-specific (RIα-icKO). Myocardial structure were evaluated by...
Abstract Introduction and objectives Left bundle branch area pacing (LBBAP), is a novel modality of physiologic that requires an adequate assessment the endocardium as well body-surface electrocardiographic signals for successful pacing-lead implantation. We aimed to assess if LBBAP criteria(1), specifically left ventricular septal (LVSP) (LBBP) criteria, can be satisfactory measured using resulting from different cardiac analyzers (PSAs) by comparing them ‘conventional’ obtained...
Can genome-wide genotyping data be analysed using a hypothesis-driven approach to enhance the understanding of genetic basis severe spermatogenic failure (SPGF) in male infertility? Our findings revealed significant association between SPGF and SHOC1 gene identified three novel genes (PCSK4, AP3B1, DLK1) along with 32 potentially pathogenic rare variants 30 that contribute this condition. is major cause infertility, often an unknown aetiology. can due either multifactorial causes, including...
Heart failure (HF) is a complex syndrome in which death rates are >50%. The main causes of among HF patients pump and ventricular arrhythmias, but severe bradycardia also common cause sudden cardiac death, pointing to sinoatrial node (SAN) dysfunction. SAN pacemaker activity regulated by voltage-clock Ca2+-clock mechanisms and, although dysfunction has been largely proved HF, remains unraveled. Here, we used an model mice with transverse aortic constriction (TAC) using telemetry, saw slower...
Abstract Cardiac ryanodine receptors (RyR2) have a key role in excitation-contraction coupling by releasing Ca2+ from sarcoplasmic reticulum (SR). In cardiomyocytes, two FK506 binding protein (FKBP) isoforms been shown to bind and stabilize RyR2 opening: FKBP12 FKBP12.6, the later having stronger affinity for despite its lower abundance. Cardiac-specific FKBP12.6 overexpressing mice fewer arrhythmias induced β-adrenergic stimulation than wild type (WT) mice, suggesting an implication of...