A5018520387- Cardiac electrophysiology and arrhythmias
- Cardiovascular and exercise physiology
- Cardiovascular Function and Risk Factors
- Cardiovascular Effects of Exercise
- Cardiomyopathy and Myosin Studies
- Nitric Oxide and Endothelin Effects
- Cardiac Ischemia and Reperfusion
- Cardiac Fibrosis and Remodeling
- Hormonal Regulation and Hypertension
- Receptor Mechanisms and Signaling
- Adipose Tissue and Metabolism
- Renin-Angiotensin System Studies
- Systemic Sclerosis and Related Diseases
- Extracellular vesicles in disease
- Exercise and Physiological Responses
- Muscle metabolism and nutrition
- Mitochondrial Function and Pathology
- Ion channel regulation and function
- Tissue Engineering and Regenerative Medicine
- Cardiovascular Disease and Adiposity
- Blood Pressure and Hypertension Studies
- Traditional Chinese Medicine Analysis
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Mass Spectrometry Techniques and Applications
- Apelin-related biomedical research
Cedars-Sinai Medical Center
2018-2025
Cedars-Sinai Smidt Heart Institute
2018-2025
Universidade Federal de Sergipe
2015-2023
Laboratoire d'Énergétique Moléculaire et Macroscopique, Combustion
2018
Inserm
2016-2018
Université Paris-Saclay
2016-2018
Université Paris-Sud
2016-2018
Instituto Politécnico Nacional
2018
Laboratory Signaling and Cardiovascular Pathophysiology
2017
Universidad Católica Nuestra Señora de la Asunción
2012
Arrhythmogenic cardiomyopathy (ACM) is characterized by progressive loss of cardiomyocytes, and fibrofatty tissue replacement. Extracellular vesicles (EVs) secreted cardiosphere-derived cells, immortalized, engineered to express high levels β-catenin, exert anti-inflammatory, anti-fibrotic effects. The aim the current study was assess efficacy EVs in an ACM murine model.
Identification and proteomic characterization of rare cell types within complex organ-derived mixtures is best accomplished by label-free quantitative mass spectrometry. High throughput required to rapidly survey hundreds thousands individual cells adequately represent populations. Here we present parallelized nanoflow dual-trap single-column liquid chromatography (nanoDTSC) operating at 15 min total run time per with peptides quantified over 11.5 using standard commercial components, thus...
The ability to increase heart rate during exercise and other stressors is a key homeostatic feature of the sinoatrial node (SAN). When physiological response blunted, chronotropic incompetence limits capacity, common problem in patients with failure preserved ejection fraction (HFpEF). Despite its clinical relevance, mechanisms remain unknown.
Kawasaki disease (KD) is an acute febrile illness and systemic vasculitis often associated with cardiac sequelae, including arrhythmias. Abundant evidence indicates a central role for IL (interleukin)-1 TNFα (tumor necrosis factor-alpha) signaling in the formation of arterial lesions KD. We aimed to investigate mechanisms underlying development electrophysiological abnormalities murine model KD vasculitis.
Myocardial fibrosis leads to cardiac dysfunction and arrhythmias in heart failure with preserved ejection fraction (HFpEF), but the underlying mechanisms remain poorly understood. Here, RNA sequencing identifies Forkhead Box1 (FoxO1) signaling as abnormal male HFpEF hearts. Genetic suppression of FoxO1 alters intercellular communication between cardiomyocytes fibroblasts, alleviates diastolic relaxation, reduces arrhythmias. Targeted downregulation activated fibroblasts fibrosis, blunts...
Resistance training is one of the most common kind exercise used nowadays. Long-term high-intensity resistance are associated with deleterious effects on vascular adjustments. On other hand, unclear whether low-intensity (LI-RT) able to induce systemic changes in tone. Thus, we aimed evaluate chronic LI-RT endothelial nitric oxide (NO) bioavailability mesenteric artery and cardiovascular autonomic modulation healthy rats. Wistar animals were divided into two groups: exercised (Ex) sedentary...
Myrtenol is a monoterpene with multiple pharmacological activities. However, although monoterpenes have been proposed to play beneficial roles in variety of cardiac disorders, actions myrtenol the heart are not yet reported. Hence, aim this study was evaluate whether promotes cardioprotection against myocardial ischemia-reperfusion (IR) injury, and mechanisms involved these effects. Male Wistar rats were orally treated for seven consecutive days (50 mg/kg) or N-acetyl cysteine (1.200 mg/kg,...
Ginkgo biloba is the most popular phytotherapic agent used worldwide for treatment of several human disorders. However, mechanisms involved in protective actions on cardiovascular diseases remain poorly elucidated. Taking into account recent studies showing beneficial cholinergic signaling heart and hypothesis biloba-mediated neuroprotection, we aimed to investigate whether extract (GBE) promotes cardioprotection via activation a model isoproterenol-induced cardiac hypertrophy. Here, show...
Overstimulation of the renin-angiotensin system (RAS) has been implicated in pathogenesis various cardiovascular diseases. Alamandine is a peptide newly identified as protective component RAS; however, mechanisms involved its beneficial effects remain elusive. By using well-characterized rat model hypertension, TGR (mREN2)27, we show that mREN ventricular myocytes are prone to contractile enhancement mediated by short-term alamandine (100 nmol/L) stimulation Mas-related G protein-coupled...
Kawasaki disease (KD), an acute febrile illness and systemic vasculitis, is the leading cause of acquired heart in children industrialized countries. KD leads to development coronary artery aneurysms (CAA) affected children, which may persist for months even years after phase disease. There unmet need characterize immune pathological mechanisms long-term complications KD. We examined cardiovascular Lactobacillus casei cell wall extract (LCWE) mouse model KD-like vasculitis over 4 months. The...
Heart failure with preserved ejection fraction (HFpEF) is defined by increased left ventricular (LV) stiffness, impaired vascular compliance, and fibrosis. Although systemic inflammation, driven comorbidities, has been proposed to play a key role, the precise pathogenesis remains elusive. To test hypothesis that inflammation drives endothelial dysfunction in HFpEF, we used cardiosphere-derived cells (CDCs), which reduce fibrosis, improving function, structure, survival HFpEF rats. Dahl...
Maturation involves a series of morphofunctional alterations vital to heart development. Its regulatory mechanisms are only now being unveiled. Evidence implies that adrenergic signaling regulates cardiac maturation, but the poorly understood. To address this point, we blocked β-ARs or performed sympathectomy followed by rescue experiments with isoproterenol in neonatal mice. Our study identifies β-AR stimuli as maturation signal for cardiomyocytes and highlights importance pathway...
Arrhythmogenic cardiomyopathy (ACM) is an inherited cardiac condition characterized by remodeling and life-threatening ventricular arrhythmias. In this issue of the JCI, Chelko, Penna, colleagues mechanistically addressed intricate contribution immune-mediated injury in ACM pathogenesis. Inhibition nuclear factor κ-B (NF-κB) infiltration monocyte-derived macrophages expressing C-C motif chemokine receptor-2 (CCR2) alleviated phenotypic features (i.e., fibrofatty replacement, contractile...
The MR (mineralocorticoid receptor) antagonists belong to the current therapeutic armamentarium for management of cardiovascular diseases, but mechanisms conferring their beneficial effects are poorly understood. Part is because regulation L-type Cav1.2 Ca2+ channel expression, which generated by tissue-specific alternative promoters as a long cardiac or short vascular N-terminal transcripts.To analyze molecular aldosterone, through MR, modulates expression and function in manner.In primary...
B1- and B2-kinin receptors are G protein-coupled that play an important role in the vascular function. Therefore, present study was designed to evaluate participation of kinin acetylcholine (ACh)-induced relaxation, focusing on protein-protein interaction involving with endothelial neuronal nitric oxide synthases (eNOS nNOS). Vascular reactivity, (NO·) reactive oxygen species (ROS) generation, co-immunoprecipitation were assessed thoracic aorta from male wild-type (WT), (B1R−/−), B2-...
◼ ion channels membrane potentialsThe American Heart Association celebrates its 100 th anniversary in 2024.
The cardiac circadian clock is responsible for the modulation of different myocardial processes, and its dysregulation has been linked to disease development. How this machinery regulated in heart remains an open question. Because noradrenaline (NE) can act as a zeitgeber cardiomyocytes, we tested hypothesis that adrenergic signaling resets gene expression vivo. In anti-phase with Clock Bmal1, Per1 abundance increased during dark phase, concurrent rise rate preceded by increase NE levels....