A5038260780- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Mast cells and histamine
- Renin-Angiotensin System Studies
- Lysosomal Storage Disorders Research
- Blood Coagulation and Thrombosis Mechanisms
- Adenosine and Purinergic Signaling
- Receptor Mechanisms and Signaling
- Genetics and Physical Performance
- Muscle metabolism and nutrition
- Nuclear Receptors and Signaling
- Autoimmune Bullous Skin Diseases
- Complement system in diseases
- Hemophilia Treatment and Research
- Renal Diseases and Glomerulopathies
- Apelin-related biomedical research
- Glycogen Storage Diseases and Myoclonus
- Histone Deacetylase Inhibitors Research
- Cardiovascular Effects of Exercise
- Vitamin K Research Studies
- Urticaria and Related Conditions
- Regulation of Appetite and Obesity
- Sports Performance and Training
- Trypanosoma species research and implications
- Hormonal Regulation and Hypertension
- Neuropeptides and Animal Physiology
Universidade Federal de São Paulo
2016-2025
Universidade Federal do Rio de Janeiro
2007-2024
Fundação de Apoio à Universidade Federal de São Paulo
2006-2024
Universidade Nove de Julho
2023
Foro Italico University of Rome
2021
Union des Industries Ferroviaires Européennes
2019
John Wiley & Sons (United States)
2019
Faculdade de Medicina de São José do Rio Preto
2018
Escola Superior de Ciências da Santa Casa de Misericórdia de Vitória
2018
Universidade Federal de Minas Gerais
2000-2015
Kinins are important mediators in cardiovascular homeostasis, inflammation, and nociception. Two kinin receptors have been described, B1 B2. The B2 receptor is constitutively expressed, its targeted disruption leads to salt-sensitive hypertension altered a heptahelical distinct from the that it highly inducible by inflammatory such as bacterial lipopolysaccharide interleukins. To clarify physiological function, we generated mice with deletion of gene for receptor. receptor-deficient animals...
The bradykinin B1-receptor is strongly upregulated under chronic inflammatory conditions. However, the mechanism and reason are not known. Because a better understanding of upregulation will help in its potential importance inflammation, we have studied molecular cultured human lung fibroblasts (IMR 90) response to IL-1beta B1-agonist [des-Arg10]-kallidin. We show that treatment IMR 90 cells by stimulates expression both mRNA protein. latter was Western blot analysis using antipeptide...
It has been described recently that the nonpeptide AVE 0991 (AVE) mimics effects of angiotensin-(1-7) [Ang-(1-7)] in bovine endothelial cells. In this study, we tested possibility is an agonist Ang-(1-7) receptor Mas, vitro and vivo. water-loaded C57BL/6 mice, (0.58 nmol/g body weight) produced a significant reduction urinary volume (0.06+/-0.03 mL/60 min [n=9] versus 0.27+/-0.05 [n=9]; P<0.01), associated with increase osmolality. The antagonist A-779 completely blocked antidiuretic effect...
Brain edema is detrimental in ischemic stroke and its treatment options are limited. Kinins proinflammatory peptides that released during tissue injury. The effects of kinins mediated by 2 different receptors (B1 B2 receptor [B1R B2R]) comprise induction formation release mediators.Focal cerebral ischemia was induced B1R knockout, B2R wild-type mice transient middle artery occlusion. Infarct volumes were measured planimetry. Evan's blue tracer applied to determine the extent brain edema....
Abstract Collapsing glomerulopathy (CG) has a severe course typically associated with viral infections, especially HIV and parvovirus B19, systemic lupus erythematosus (SLE), among other etiologies. A 35-year-old woman recent use of JAK inhibitor due to rheumatoid arthritis presented 2-week history fever, cervical adenopathy, facial erythema. After admission, anemia, hypoalbuminemia, proteinuria, acute kidney injury were noted. SLE was diagnosed B19 DNA detected in serum samples. Kidney...
Open reading frame expressed sequences tags (ORESTES) differ from conventional ESTs by providing sequence data the central protein coding portion of transcripts. We generated a total 696,745 ORESTES 24 human tissues and used subset that correspond to set 15,095 full-length mRNAs as means assessing efficiency strategy its potential contribution definition transcriptome. estimate sampled over 80% all highly moderately expressed, between 40% 50% rarely genes. In our most thoroughly sequenced...
Renin-angiotensin system is involved in homeostasis processes linked to renal and cardiovascular recently has been metabolic syndrome. We analyzed the influence of long term angiotensin I converting enzyme (ACE) inhibitor enalapril treatment normotensive adult Wistar rats fed with standard or palatable hyperlipidic diets. Our results show that decreases absolute food intake, serum leptin concentration body weight gain. Moreover, adipose tissue, led decreased ACE activity, enhanced expression...
Abstract Post-exercise hypotension is an important event for blood pressure regulation, especially in hypertensive individuals. Although post-exercise a well-known phenomenon, the mechanism responsible still unclear. The kallikrein-kinin system involved control, but its role has not yet been investigated. Thus, purpose of this study was to investigate involvement vasodilators bradykinin and des-Arg 9 -BK kallikrein activity promoted by 35 min cycle ergometer (CE) or circuit weight-training...
To investigate the antihypertensive effects of conventional resistance exercise (RE) on blood pressure (BP) hypertensive subjects, 15 middle-aged (46 ± 3 years) volunteers, deprived medication (reaching 153 6/93 2 mm Hg systolic/diastolic BP after a 6-week washout period) were submitted to 12-week RE training program (3 sets 12 repetitions at 60% 1 repetition maximum, times week nonconsecutive days). Blood was measured in all phases study (washout, training, detraining). Additionally, plasma...
To evaluate the cardiovascular actions of kinins, we established a transgenic rat line harboring human tissue kallikrein gene, TGR(hKLK1). Under control zinc-inducible metallothionein promoter, transgene was expressed in most tissues including heart, kidney, lung, and brain, detected urine animals. Transgenic rats had lower 24-h mean arterial pressure comparison with rats, which further decreased when their diet supplemented zinc. The day/night rhythm blood significantly diminished...
Kinins play a central role in the modulation of cardiovascular function and pathophysiology inflammation. These peptides mediate their effects by binding to two specific G-protein coupled receptors named B1 B2. To evaluate full functional relevance kallikrein-kinin system, we generated mice lacking both kinin (B1B2-/-). Because close chromosomal position receptor genes, B1B2-/- could not be obtained simple breeding single knockout lines. Therefore, inactivated gene homologous recombination...
Diabetic cardiomyopathy includes fibrosis. Kallikrein (KLK) can inhibit collagen synthesis and promote breakdown. We investigated cardiac fibrosis left ventricular (LV) function in transgenic rats (TGR) expressing the human kallikrein 1 (hKLK1) gene streptozotocin (STZ) -induced diabetic conditions. Six weeks after STZ injection, LV was determined male Sprague-Dawley (SD) TGR(hKLK1) (n=10/group) by a Millar tip catheter. Total content (Sirius Red staining) expression of types I, III, VI were...
Although the concept that dendritic cells (DCs) recognize pathogens through engagement of Toll-like receptors is widely accepted, we recently suggested immature DCs might sense kinin-releasing strains Trypanosoma cruzi triggering G-protein-coupled bradykinin B2 (B2R). Here report C57BL/6.B2R−/− mice infected intraperitoneally with T. display higher parasitemia and mortality rates as compared to B2R+/+ mice. qRT-PCR revealed a 5-fold increase in DNA (14 d post-infection [p.i.]) B2R−/− heart,...
Abstract The kinin B1 receptor is an inducible not normally expressed but induced by inflammatory stimuli and plays a major role in neutrophil recruitment, particularly response to the cytokine IL-1β. However, exact mechanism involved this unclear. aim of study was dissect molecular involved, particular determine whether specific ELR-CXCL chemokines (specific chemoattractants) played role. Using intravital microscopy, we demonstrated that IL-1β-induced leukocyte rolling, adherence,...
Abstract We have previously reported that exogenous bradykinin activates immature dendritic cells (DCs) via the B2 receptor (B2R), thereby stimulating adaptive immunity. In this study, we show these premises are met in a model of s.c. infection by Trypanosoma cruzi, protozoan liberates kinins from kininogens through its major protease, cruzipain. Intensity B2R-dependent paw edema evoked trypomastigotes correlated with levels IL-12 produced CD11c+ isolated draining lymph nodes. The response...
Abstract Background Systemic therapy for cancer metastatic lesions is difficult and generally renders a poor clinical response. Structural analogs of cisplatin, the most widely used synthetic metal complexes, show toxic side-effects tumor cell resistance. Recently, palladium complexes with increased stability are being investigated to circumvent these limitations, biphosphinic cyclopalladated complex {Pd 2 [ S (-) C , N-dmpa] (μ-dppe)Cl } named C7a efficiently controls subcutaneous...
Resumo A glomerulopatia colapsante (GC) apresenta um curso grave, tipicamente associado a infecções virais, especialmente HIV e parvovírus B19, lúpus eritematoso sistêmico (LES), entre outras etiologias. Uma mulher de 35 anos, com uso recente inibidor JAK devido à artrite reumatoide, apresentou histórico duas semanas febre, adenopatia cervical eritema facial. Após admissão, observou-se anemia, hipoalbuminemia, proteinúria injúria renal aguda grave. Foi diagnosticado LES o DNA do B19 foi...