A5010577496- Thyroid Cancer Diagnosis and Treatment
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Thyroid Disorders and Treatments
- S100 Proteins and Annexins
- Cancer-related Molecular Pathways
- TGF-β signaling in diseases
- Growth Hormone and Insulin-like Growth Factors
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Circular RNAs in diseases
- Science and Science Education
- Inflammatory mediators and NSAID effects
- Connective Tissue Growth Factor Research
- Galectins and Cancer Biology
- Estrogen and related hormone effects
- Biomarkers in Disease Mechanisms
- Business and Management Studies
- Molecular Biology Techniques and Applications
- Epigenetics and DNA Methylation
- BRCA gene mutations in cancer
- Glutathione Transferases and Polymorphisms
- Hedgehog Signaling Pathway Studies
- Education and Public Policy
Universidade de São Paulo
2016-2025
Instituto Butantan
2014-2020
Hospital Universitário da Universidade de São Paulo
2013
Instituto Nacional de Cardiología
2012
Brazilian Medical Association
2010
Ordem dos Médicos
2010
University of Cincinnati Medical Center
2003-2009
Universidade Brasil
2007
Universidad Nacional de Córdoba
2006
University of Cincinnati
2003
Abstract Activating point mutations of the BRAF gene have been recently reported in papillary thyroid carcinomas. In this study, we analyzed 320 tumors and six anaplastic carcinoma cell lines detected 45 (38%) carcinomas, two (13%) poorly-differentiated three (10%) five (83%) but not follicular, Hürthle cell, medullary follicular adenomas, or benign hyperplastic nodules. All involved a T→A transversion at nucleotide 1796. were associated with older age, classic tall variant histology,...
Leptospira species colonize a significant proportion of rodent populations worldwide and produce life-threatening infections in accidental hosts, including humans. Complete genome sequencing interrogans serovar Copenhageni comparative analysis with the available Lai reveal that despite overall genetic similarity there are structural differences, large chromosomal inversion extensive variation number distribution insertion sequence elements. Genome elucidates many novel aspects leptospiral...
Xylella fastidiosa is a xylem-dwelling, insect-transmitted, gamma-proteobacterium that causes diseases in many plants, including grapevine, citrus, periwinkle, almond, oleander, and coffee. X. has an unusually broad host range, extensive geographical distribution throughout the American continent, induces diverse disease phenotypes. Previous molecular analyses indicated three distinct groups of isolates were expected to be genetically divergent. Here we report genome sequence (Temecula...
The Dio2 gene encodes the type 2 deiodinase (D2) that activates thyroxine (T4) to 3,3′,5-triiodothyronine (T3), disruption of which (Dio2−/−) results in brown adipose tissue (BAT)-specific hypothyroidism an otherwise euthyroid animal. In present studies, cold exposure increased Dio2−/− BAT sympathetic stimulation ∼10-fold (normal ∼4-fold); as a result, lipolysis, well mRNA levels uncoupling protein 1, guanosine monophosphate reductase, and peroxisome proliferator–activated receptor γ...
Coffee is one of the most valuable agricultural commodities and ranks second on international trade exchanges. The genus Coffea belongs to Rubiaceae family which includes other important plants. contains about 100 species but commercial production based only two species, arabica canephora that represent 70 % 30 total coffee market, respectively. Brazilian Genome Project was designed with objective making modern genomics resources available scientific community, working different aspects...
We developed mice with germline endogenous expression of oncogenic Hras to study effects on development and mechanisms tumor initiation. They had high perinatal mortality, abnormal cranial dimensions, defective dental ameloblasts, nasal septal deviation, consistent some the features human Costello syndrome. These papillomas angiosarcomas, which were associated G12V allelic imbalance augmented signaling. Endogenous was also a higher mutation rate in vivo. Tumor initiation by likely requires...
Open reading frame expressed sequences tags (ORESTES) differ from conventional ESTs by providing sequence data the central protein coding portion of transcripts. We generated a total 696,745 ORESTES 24 human tissues and used subset that correspond to set 15,095 full-length mRNAs as means assessing efficiency strategy its potential contribution definition transcriptome. estimate sampled over 80% all highly moderately expressed, between 40% 50% rarely genes. In our most thoroughly sequenced...
The genome sequence of Leifsonia xyli subsp. xyli, which causes ratoon stunting disease and affects sugarcane worldwide, was determined. single circular chromosome CTCB07 2.6 Mb in length with a GC content 68% 2,044 predicted open reading frames. analysis also revealed 307 pseudogenes, is more than any bacterial plant pathogen sequenced to date. Many these if functional, would likely be involved the degradation heteropolysaccharides, uptake free sugars, synthesis amino acids. Although L. has...
Papillary thyroid carcinoma (PTC) is the most common endocrine malignancy and RET/PTC rearrangements represent key genetic events frequently associated to this cancer, enhancing proliferation dedifferentiation by activation of RET/PTC-RAS-BRAF-mitogen-activated protein kinase (MAPK) pathway. Recently, let-7 microRNA was found reduce RAS levels in lung acting as a tumor suppressor gene. Here, we report that RET/PTC3 oncogenic PCCL3 rat cells markedly reduces let-7f expression. Moreover,...
Tumor invasiveness is directly related to the ability of tumor cells migrate and invade surrounding tissues, usually degrading extracellular matrix. Despite significant progress in knowledge about migration invasion, there much more elucidate their regulatory mechanisms, especially cancer cells. MicroRNAs (miRs) were recently described as important regulators migration. Differential expression miRs frequently associated with progression, invasion metastasis. In papillary thyroid carcinoma...
Mutually exclusive genetic alterations in the RET, RAS, or BRAF genes, which result constitutively active mitogen-activated protein kinase (MAPK) signaling, are present about 70% of papillary thyroid carcinomas (PTCs). However, effect MAPK activation on other signaling pathways involved oncogenic transformation, such as Notch, remains unclear. In this study, we tested hypothesis that pathway regulates Notch and plays a role PTC cell proliferation. Conditional induction oncogenes RET/PTC3...
Although somatic mutations have been identified in a subset of thyroid nodules, the pathogenesis nodules multinodular goiters remains unclear. Clonal analysis indicates whether nodule arises from polyclonal proliferation group cells or forms clone genetically altered cell. Individual shown to be monoclonal origin. In this study we examined clonality several different patients with goiters. Clonality was established using X-chromosomal probe M27 beta, which detects multiallelic polymorphism...
Galectin-3 is a protein of the lectin family that has been associated with neoplastic processes in various tissues. In thyroid, expression this described differentiated follicular cancer, suggesting immunohistochemical study galectin-3 may be potential marker malignancy thyroid neoplasms. The confirmation these results represent an extremely useful tool for presurgical diagnosis and medical conduct. study, mRNA were analyzed tissues from 87 patients histomorphological multinodular goiter...
As judged by the response of uncoupling protein and key enzymes, brown adipose tissue (BAT) is highly dependent upon local generation T3 catalyzed type II T4 5′-deiodinase (5′D-II). In hypothyroid rats treated with or T4, capacity to withstand cold seems better correlated normalization BAT responses than liver thyroid status. 5′D-II activated via sympathetic nervous system (SNS) stimulation, activation generates enough nearly saturate nuclear receptors (NTR) in euthyroid rats....
The phenomenon that supraphysiological doses of iodide (I(-)) temporarily inhibit thyroid hormone synthesis is known as autoregulation. Recovery function has been attributed to sodium-iodide symporter (NIS) inhibition, but the diversity available data makes it difficult reach definitive conclusions. Iodide excess induces reactive oxygen species production and cell toxicity. However, roles oxidative state antioxidant selenoproteins in I(-) autoregulation have never explored. Here we analyze...
Excess iodine inhibits thyroid follicular cell proliferation associated with TGFβ pathway activation, although cancers are frequently refractory to signaling. The is predicted be regulated by miR-17-92 cluster microRNAs. MicroRNAs small noncoding RNAs that inhibit target mRNA translation and have emerged as potent modulators of tumorigenesis. Although the BRAF(V600E) mutation most prevalent alteration in cancer, impact intake on BRAF-mediated oncogenesis remains unclear. Therefore, aim this...
// Murilo Vieira Geraldo 1, 3 , Helder Imoto Nakaya 2 Edna Teruko Kimura 1 Department of Cell and Developmental Biology, Institute Biomedical Sciences, University Sao Paulo, Brazil Clinical Analyses Toxicology, School Pharmaceutical Structural Functional State Campinas, Correspondence to: Geraldo, email: murilovg@unicamp.br Kimura, etkimura@usp.br Keywords: microRNA, papillary thyroid carcinoma, 14q32 region, microTranscriptome, metastasis Received: September 18, 2016 Accepted: November 26,...