A5056102443- Phytoplasmas and Hemiptera pathogens
- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Cocoa and Sweet Potato Agronomy
- Insect symbiosis and bacterial influences
- Plant Virus Research Studies
- Legume Nitrogen Fixing Symbiosis
- Plant Physiology and Cultivation Studies
- Plant Reproductive Biology
- Banana Cultivation and Research
- Pineapple and bromelain studies
- Plant Pathogens and Fungal Diseases
- Bacterial biofilms and quorum sensing
- Plant Parasitism and Resistance
- Mycorrhizal Fungi and Plant Interactions
- Plant pathogens and resistance mechanisms
- Insect Resistance and Genetics
- Plant Disease Resistance and Genetics
- Insect-Plant Interactions and Control
- CRISPR and Genetic Engineering
- Studies on Chitinases and Chitosanases
- Transgenic Plants and Applications
- Mosquito-borne diseases and control
- Bacteriophages and microbial interactions
- Entomopathogenic Microorganisms in Pest Control
Anhanguera (Brazil)
2007-2024
Agronomical Institute of Campinas
2015-2024
Pontifícia Universidade Católica de Goiás
2024
Universidade Federal de Goiás
2024
Universidad Católica de Santa Fe
2024
Instituto Agronômico do Paraná
2016-2024
Universidade Estadual de Montes Claros
2020
IAC (United States)
1999-2019
Universidade Estadual de Campinas (UNICAMP)
2003-2018
Universidade Federal de São Carlos
2008-2018
Xylella fastidiosa is a xylem-dwelling, insect-transmitted, gamma-proteobacterium that causes diseases in many plants, including grapevine, citrus, periwinkle, almond, oleander, and coffee. X. has an unusually broad host range, extensive geographical distribution throughout the American continent, induces diverse disease phenotypes. Previous molecular analyses indicated three distinct groups of isolates were expected to be genetically divergent. Here we report genome sequence (Temecula...
Biofilms are complex microbial communities with important biological functions including enhanced resistance against external factors like antimicrobial agents. The formation of a biofilm is known to be strongly dependent on substrate properties hydrophobicity/hydrophilicity, structure, and roughness. adsorption (macro)molecules the substrate, also as conditioning film, changes physicochemical surface affects bacterial adhesion. In this study, we investigate caused by Periwinkle wilt (PW)...
Abstract Microorganism pathogenicity strongly relies on the generation of multicellular assemblies, called biofilms. Understanding their organization can unveil vulnerabilities leading to potential treatments; spatially and temporally-resolved comprehensive experimental characterization provide new details biofilm formation possibly targets for disease control. Here, economically important phytopathogen Xylella fastidiosa was analyzed at single-cell resolution using nanometer-resolution...
The genome sequence of Leifsonia xyli subsp. xyli, which causes ratoon stunting disease and affects sugarcane worldwide, was determined. single circular chromosome CTCB07 2.6 Mb in length with a GC content 68% 2,044 predicted open reading frames. analysis also revealed 307 pseudogenes, is more than any bacterial plant pathogen sequenced to date. Many these if functional, would likely be involved the degradation heteropolysaccharides, uptake free sugars, synthesis amino acids. Although L. has...
The complete nucleotide sequence of the genomic RNA 1 (8745 nt) and 2 (4986 Citrus leprosis virus cytoplasmic type (CiLV-C) was determined using cloned cDNA. contains two open reading frames (ORFs), which correspond to 286 29 kDa proteins. protein is a polyprotein putatively involved in replication, four conserved domains: methyltransferase, protease, helicase polymerase. ORFs corresponding 15, 61, 32 24 proteins, respectively. apparently cell-to-cell movement virus, but none other putative...
Citrus fruits (sweet orange, mandarin, pummelo, grapefruit, lemon, lime and assorted hybrids) are among the most widely grown economically important fruit tree crops in world. As fresh fruit, they an nutrient dense food source for human diets; as processed juice products, primarily sweet orange juice, represent a globally traded commodity. To support genetic improvement efforts this crop, international citrus genetics community has collaborated with sequencing centers development of freely...
Xylella fastidiosa is a plant pathogen bacterium that causes diseases in many different crops. In citrus, it Citrus Variegated Chlorosis (CVC). The mechanism of pathogenicity this associated with its capacity to colonize and form biofilm the xylem vessels host plants, there not yet any method directly reduce populations field. study, we investigated inhibitory effect N-Acetylcysteine (NAC), cysteine analogue used mainly treat human diseases, on X. experimental conditions. Concentrations NAC...
Abstract Background Citrus variegated chlorosis (CVC), caused by Xylella fastidiosa , is one the most important citrus diseases, and affects all varieties of sweet orange ( sinensis L. Osb). On other hand, among genus there are different sources resistance against X. . For these species identifying defense genes could be an step towards obtaining resistant through breeding or genetic engineering. To assess we made use mandarin C. reticulata Blanco) that known to CVC shares agronomical...
Surface attachment of a planktonic bacteria, mediated by adhesins and extracellular polymeric substances (EPS), is crucial step for biofilm formation. Some pathogens can modulate cell adhesiveness, impacting host colonization virulence. A framework able to quantify cell-surface interaction forces their dependence on chemical surface composition may unveil adhesiveness control mechanisms as new targets intervention disease control. Here we employed InP nanowire arrays dissect factors involved...
Citrus cropping is widely distributed as an important economic activity worldwide. Amongst the major producers are Brazil, China, United States, Mexico and some European countries. Brazil largest sweet orange producer accounting for more than three-quarters of juice exports around world, followed by China States (Foreign Agricultural Service/USDA, 2019). Although main commodity in many countries producing citrus, by-products like essential oils, molasses, dried pulp, pectin, blend syrup...
Citrus agribusiness faces major economic losses due to bacterial diseases (Caserta et al., 2020). canker (CC) and citrus variegated chlorosis (CVC) caused by Xanthomonas citri subsp. (Xcc) Xylella fastidiosa pauca (Xfp), respectively, are important threats in commercial orchards. All sweet orange (Citrus sinensis) varieties susceptible both pathogens, no natural resistance has been found so far. Plant cell-surface receptors recognize pathogen (or microbe)-associated molecular patterns...
The aims of this study were to develop simple sequence repeat markers (SSRs or microsatellite markers) in citrus and evaluate the efficiency these for characterization sweet orange. We developed SSRs from a genomic library 'Pêra IAC' orange enriched AG/TC, GT/CA, TCA/AGT AAC/TTG repeats. selected 279 sequences which 171 primer pairs designed 113 with best banding patterns selected. Characterization loci revealed that AG/TC was most abundant (69%) class isolated, followed by GT/CA (15.9%),...
A biofilm is a community of microorganisms attached to solid surface. Cells within biofilms differ from planktonic cells, showing higher resistance biocides, detergent, antibiotic treatments and host defense responses. Even though there are number gene expression studies in bacterial formation, limited information available concerning plant pathogen. It was previously demonstrated that the pathogen Xylella fastidiosa could grow as biofilm, possibly important factor for its pathogenicity. In...
The phytopathogen Xylella fastidiosa grows as a biofilm causing vascular occlusion and consequently nutrient water stress in different plant hosts by adhesion on xylem vessel surfaces composed of cellulose, hemicellulose, pectin proteins. Understanding the factors which influence bacterial development is key issue identifying mechanisms for preventing formation infected plants. In this study, we show that X. architecture correlate well with physicochemical surface properties after...
Through the formation of persister cells, bacteria exhibit tolerance to multidrug and other environmental stresses without undergoing genetic changes. The toxin-antitoxin (TA) systems are involved in cells because they able induce cell dormancy. Among TA systems, MqsRA system has been observed be highly induced Xylella fastidiosa (causal agent citrus variegated chlorosis – CVC) activated by copper stress, described Escherichia coli as related biofilms. Thus, we evaluated role this X....
Resistance inducers have been used in annual crops as an alternative for disease control. Wood perennial fruit trees, such those of the citrus species, are candidates treatment with resistance inducers, salicylic acid (SA) and chitosan (CHI). However, involved mechanisms induced by elicitors currently few known. In present manuscript, we report information regarding transcriptional changes observed sweet orange response to exogenous applications SA CHI using RNA-seq technology. More genes...
The landscape of scientific publishing is experiencing a transformative shift toward open access, paradigm that mandates the availability research outputs such as data, code, materials, and publications. Open access provides increased reproducibility allows for reuse these resources. This article guidance best practices research, associated resources, including in American Phytopathological Society journals. Key areas diagnostic assays, experimental design, data sharing, code deposition are...
The Citrus sinensis LATERAL ORGAN BOUNDERIES 1 (CsLOB1) gene, which is directly induced by the Xanthomonas citri subsp. effector PthA4, functions as a transcription factor and citrus canker susceptibility (S) gene. Genome editing of CsLOB1 has been shown to confer resistance disease. Previous studies revealed that CsLOB1-INDUCED EXPANSIN gene (CsLIEXP1) highly upregulated in citri-infected plants. Because expansins are associated with cell wall loosening, potentially facilitating bacterial...