A5028039856- Plant Pathogenic Bacteria Studies
- Legume Nitrogen Fixing Symbiosis
- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Fungal Diseases
- Banana Cultivation and Research
- Plant Disease Resistance and Genetics
- Genomics and Phylogenetic Studies
- Vibrio bacteria research studies
- Infections and bacterial resistance
- Biofuel production and bioconversion
- Plant Physiology and Cultivation Studies
- Plant Virus Research Studies
- Microbial Metabolism and Applications
- Polyamine Metabolism and Applications
- Phytoplasmas and Hemiptera pathogens
- Photosynthetic Processes and Mechanisms
- Insect symbiosis and bacterial influences
- Research Data Management Practices
- Irrigation Practices and Water Management
- Salmonella and Campylobacter epidemiology
- Education Systems and Policy
- Insect and Pesticide Research
- SARS-CoV-2 detection and testing
- Insect and Arachnid Ecology and Behavior
- Irish and British Studies
University of California, Davis
2020-2025
Plant (United States)
2015-2024
University of Wisconsin–Madison
2014-2023
University of California System
2022
University of California, Berkeley
2018-2021
Baylor University
2021
Georgia Institute of Technology
2015
Ralstonia solanacearum thrives in plant xylem vessels and causes bacterial wilt disease despite the low nutrient content of sap. We found that R. manipulates its host to increase nutrients tomato sap, enabling it grow better sap from infected plants than healthy plants. Untargeted GC/MS metabolomics identified 22 metabolites enriched solanacearum-infected Eight these could serve as sole carbon or nitrogen sources for solanacearum. Putrescine, a polyamine is not source solanacearum, was...
Ralstonia solanacearum is a globally distributed soil-borne plant pathogenic bacterium, which shares broad ecological range with many plant- and soil-associated fungi. We sought to determine if R. chemical communication directs symbiotic development of polymicrobial consortia. produced diffusible metabolite that induced conserved morphological differentiation in 34 species fungi across three diverse taxa (Ascomycetes, Basidiomycetes Zygomycetes). Fungi exposed this formed chlamydospores,...
ABSTRACT The PhcA virulence regulator in the vascular wilt pathogen Ralstonia solanacearum responds to cell density via quorum sensing. To understand timing of traits that enable R. establish itself inside host plants, we created a Δ phcA mutant is genetically locked low-cell-density condition. Comparing levels gene expression wild-type and during tomato colonization revealed transcriptome includes an impressive 620 genes (>2-fold differentially expressed; false-discovery rate [FDR],...
Ralstonia solanacearum is a vascular soil-borne plant pathogen with an unusually broad host range. This economically destructive and globally distributed bacterium has thousands of distinct lineages within heterogeneous taxonomically disputed species complex. Some include highly host-adapted strains (ecotypes), such as the banana Moko disease-causing strains, cold-tolerant potato brown rot (also known R3bv2) recently emerged Not Pathogenic to Banana (NPB) strains. These ecotypes offer robust...
ABSTRACT Plants use the signaling molecule salicylic acid (SA) to trigger defenses against diverse pathogens, including bacterial wilt pathogen Ralstonia solanacearum . SA can also inhibit microbial growth. Most sequenced strains of heterogeneous R. species complex degrade via gentisic pyruvate and fumarate. strain GMI1000 expresses this degradation pathway during tomato pathogenesis. Transcriptional analysis revealed that subinhibitory levels induced expression pathway, toxin efflux pumps,...
Plants produce hydroxycinnamic acid (HCA) defense compounds to combat pathogens, such as the bacterium Ralstonia solanacearum. We showed that an HCA degradation pathway is genetically and functionally conserved across diverse R. solanacearum strains. Further, a feruloyl-CoA synthetase (Δfcs) mutant cannot degrade was less virulent on tomato plants. To understand role of in bacterial wilt disease, we tested following hypotheses: helps pathogen i) grow, carbon source; ii) spread, by reducing...
Ralstonia causes wilt diseases by colonizing xylem vessels and disrupting water transport. The dogma is that bacterial biomass clogs reduces the flow of sap due to abundance. However, physiological mechanism disruption during untested. Using a tomato pseudosolanacearum GMI1000 model, we visualized quantified spatiotemporal dynamics wilt. First, measured stomatal conductance leaflets on mock-inoculated wilt-symptomatic plants. Wilted had reduced conductance, as did turgid same petiole wilted...
The landscape of scientific publishing is experiencing a transformative shift toward open access, paradigm that mandates the availability research outputs such as data, code, materials, and publications. Open access provides increased reproducibility allows for reuse these resources. This article guidance best practices research, associated resources, including in American Phytopathological Society journals. Key areas diagnostic assays, experimental design, data sharing, code deposition are...
Ginger (Zingiber officinale) is an herbaceous perennial in the Zingiberaceae family grown primarily tropical to subtropical biomes as a culinary spice, traditional medicine, and landscaping plant. While ginger grows at soil temperatures above 20°C, several farmers upper Midwestern US short-season high tunnels. In 2023 2024, growers southeastern Minnesota reported new disease of ginger. USDA traced origin rhizomes imported by these Peru (Soto-Heredia et al., 2024). Plants exhibited wilting,...
'The integration of mathematical modeling and experimental data allows researchers to investigate infection conditions that are difficult achieve in reality.' How does Ralstonia acquire sufficient carbon for its massive planta reproduction? To answer questions such as this, bacteriologists typically turn our favorite approaches, bioassays with metabolic mutants, comparative genomics, gene expression analysis, occasionally, chemistry, if we must. Molecular genetics provide evidence sugars...
The Ralstonia solanacearum species complex (RSSC) is a globally distributed group of gram-negative, soil-borne bacteria that cause wilt disease on broad range host plant species. Tomato widely cultivated and economically important crop in Tanzania, but bacterial caused by the RSSC has emerged to be one most devastating diseases tomato producing regions Tanzania. To investigate genomic diversity strains isolated from we generated draft genomes 19 isolates 5 villages 4 regions. Our...
Ralstonia solanacearum species complex pathogens cause bacterial wilt disease in diverse plant families. These use chemotaxis and motility to discover host roots. The specificity of this process is conferred by methyl-accepting proteins (MCPs). We explored pangenomic variation MCPs other machinery across pathogens. classified 19 as broadly conserved, core MCPs, we identified several accessory MCPs. Within their periplasmic sensing domains, two the contain a motif that known bind amino acid...
Virulence assays are powerful tools to study microbial pathogenesis in vivo. Good track disease development and, coupled with targeted mutagenesis, can identify pathogen virulence factors. Disease plants is extremely sensitive environmental factors such as temperature, atmospheric humidity, and soil water level, so it be challenging standardize conditions achieve consistent results. Here, we present optimized validated experimental analysis methods for nine that measure specific aspects of...
Background. Ralstonia solanacearum is an economically important plant pathogen with unusually large host range. The Moko (banana) and NPB (not pathogenic to banana) strain groups are closely related but adapted distinct hosts. Previous comparative genomics studies uncovered very few differences that could account for the range difference between these pathotypes. To better understand basis of this specificity, we used RNAseq profile transcriptomes R. under in vitro planta conditions....
Vibrio parahaemolyticus is an aquatic halophilic bacterium that occupies estuarine and coastal marine environments, a leading cause of seafood-borne food poisoning cases. To investigate the environmental reservoir potential gene flow occurs among V. isolates, virulence-associated content genome diversity collection 133 isolates were analyzed. Phylogenetic analysis housekeeping genes, pulsed-field gel electrophoresis, demonstrated there genetic similarity clinical isolates. Whole-genome...
Expansin proteins, which loosen plant cell walls, play critical roles in normal growth and development. The horizontal acquisition of functional plant-like expansin genes numerous xylem-colonizing phytopathogenic bacteria suggests that bacterial expansins may also contribute to virulence. To investigate the role diseases, we mutated non-chimeric (CmEXLX2 RsEXLX) two xylem-inhabiting pathogens, Actinobacterium Clavibacter michiganensis ssp. (Cmm) β-proteobacterium Ralstonia solanacearum (Rs),...
The small polyamine putrescine (1,4-diaminobutane) is ubiquitously and abundantly found in all three domains of life. It a precursor, through N-aminopropylation or N-aminobutylation, for biosynthesis the longer polyamines spermidine, sym-homospermidine, spermine, thermospermine branched chain polyamines. Putrescine also biochemically modified purposes metabolic regulation catabolism, e.g. N-acetylation N-glutamylation, incorporation into specialized metabolites, N-methylation, N-citrylation,...
Bacterial pathogens in the Ralstonia solanacearum species complex (RSSC) infect water-transporting xylem vessels of plants, causing bacterial wilt disease. Strains RSSC phylotypes I and III can reduce nitrate to dinitrogen via complete denitrification. The four-step denitrification pathway enables bacteria use inorganic nitrogen as terminal electron acceptors, supporting their growth oxygen-limited environments such biofilms or plant xylem. Reduction nitrate, nitrite, nitric oxide all...
Abstract The Ralstonia species complex is a group of genetically diverse plant wilt pathogens. Our goal to create database that contains the reported global distribution and host range clades (e.g. phylotypes sequevars). In this fifth release, we have cataloged information from 304 sources report one or more strains isolated 107 geographic regions. Metadata for nearly 10,000 are available as supplemental table. aggregated data suggest pandemic brown rot lineage (IIB-1) most widely dispersed...
Plant-pathogenic Ralstonia spp. colonize plant xylem and cause wilt diseases on a broad range of host plants. To identify genes that promote growth diverse strains in sap from tomato plants, we performed genome-scale genetic screens (random barcoded transposon mutant sequencing [RB-TnSeq]) three spanning the genetic, geographical, physiological plant-pathogenic Ralstonia: solanacearum IBSBF1503, pseudosolanacearum GMI1000, syzygii PSI07. Contrasting fitness phenotypes culture media versus...
Abstract Colleges and universities around the world engaged diverse strategies during COVID-19 pandemic. Baylor University, a community of ˜22,700 individuals, was 1 institutions which resumed sustained operations. The key strategy establishment multidisciplinary teams to develop mitigation priority areas for action. This population-based team approach along with implementation “Swiss Cheese” risk model allowed small clusters be rapidly addressed through testing, surveillance, tracing,...