A5077823769- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Plant Virus Research Studies
- Genomics and Phylogenetic Studies
- Plant Molecular Biology Research
- Chromosomal and Genetic Variations
- Cassava research and cyanide
- Plant Disease Resistance and Genetics
- Plant pathogens and resistance mechanisms
- Photosynthetic Processes and Mechanisms
- Plant responses to water stress
- Banana Cultivation and Research
- Genetic and Environmental Crop Studies
- Calcium signaling and nucleotide metabolism
- Vibrio bacteria research studies
- Plant Genetic and Mutation Studies
- Peanut Plant Research Studies
- Plant tissue culture and regeneration
- Cellular transport and secretion
- Silk-based biomaterials and applications
- Rice Cultivation and Yield Improvement
- Genetic Mapping and Diversity in Plants and Animals
- Error Correcting Code Techniques
- Advanced Data Storage Technologies
Centre de Coopération Internationale en Recherche Agronomique pour le Développement
2015-2024
Centre Occitanie-Montpellier
2021-2024
Plant Health Institute de Montpellier
2021-2024
Université de Montpellier
2015-2023
Institut de Recherche pour le Développement
2013-2023
Institut Agro Montpellier
2021-2023
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2021-2023
Colorado State University
2018-2021
Inserm
2017-2019
Institut de Biologie de l'École Normale Supérieure
2017-2019
Abstract Bacterial blight of rice is an important disease in Asia and Africa. The pathogen, Xanthomonas oryzae pv. ( Xoo ), secretes one or more six known transcription-activator-like effectors (TALes) that bind specific promoter sequences induce, at minimum, the three host sucrose transporter genes SWEET11 , SWEET13 SWEET14 expression which required for susceptibility. We used CRISPR–Cas9-mediated genome editing to introduce mutations all SWEET gene promoters. Editing was further informed...
Transcription Activators-Like Effectors (TALEs) belong to a family of virulence proteins from the Xanthomonas genus bacterial plant pathogens that are translocated into cell. In nucleus, TALEs act as transcription factors inducing expression susceptibility genes. A code for TALE-DNA binding specificity and high-resolution three-dimensional structures complexes were recently reported. Accurate prediction TAL Effector Binding Elements (EBEs) is essential elucidate biological functions many...
A single gene with a dynamic evolutionary history allows plant pathogenic bacteria to spread systemically through their hosts.
Abstract Background microRNAs (miRNAs) are non-coding short RNAs that regulate gene expression in eukaryotes by translational inhibition or cleavage of complementary mRNAs. In plants, miRNAs known to target mostly transcription factors and implicated diverse aspects plant growth development. A role has been suggested for the miRNA pathway antiviral defense plants. this work, a bioinformatics approach was taken test whether from six species could have activity targeting genomes infecting...
Abstract Background microRNAs (miRNAs) are short RNA molecules that control gene expression by silencing complementary mRNA. They play a crucial role in stress response plants, including biotic stress. Some miRNAs known to respond bacterial infection Arabidopsis thaliana but it is currently unknown whether these responses conserved other plants and novel species-specific could have defense. Results This work addresses the of Manihot esculenta (cassava)- Xanthomonas axonopodis pv. manihotis...
Understanding the processes that shaped contemporary pathogen populations in agricultural landscapes is quite important to define appropriate management strategies and support crop improvement efforts. Here, we took advantage of an historical record examine adaptation pathway rice Xanthomonas oryzae pv. (Xoo) a semi-isolated environment represented Philippine archipelago. By comparing genomes key Xoo groups showed modern derived from three Asian lineages. We also diversification virulence...
Transcription Activator-Like (TAL) effectors from Xanthomonas plant pathogenic bacteria can bind to the promoter region of genes and induce their expression. DNA-binding specificity is governed by a central domain made nearly identical repeats, each determining recognition one base pair via two amino acid residues (a.k.a. Repeat Variable Di-residue, or RVD). Knowing how TAL differ other within between strains would be useful infer functional evolutionary relationships, but repetitive nature...
Abstract AvrE family type III effector proteins share the ability to suppress host defenses, induce disease-associated cell death, and promote bacterial growth. However, despite widespread contributions numerous diseases in agriculturally important plants, mode of action these effectors remains largely unknown. WtsE is an member required for Pantoea stewartii ssp. (Pnss) proliferate efficiently cause wilt leaf blight symptoms maize (Zea mays) plants. Notably, when delivered by a heterologous...
Xanthomonas axonopodis pv. manihotis (Xam) is the causal agent of bacterial blight cassava, which among main components human diet in Africa and South America. Current information about molecular pathogenicity factors involved infection process this organism limited. Previous studies other bacteria genus suggest that advanced draft genome sequences are valuable resources for on their interaction with plants could provide tools diagnostics detection. Here we have generated first manually...
Many plant-pathogenic bacteria suppress pathogen-associated molecular pattern (PAMP)-triggered immunity by injecting effector proteins into the host cytoplasm during infection through type III secretion system (TTSS). This secretome plays an important role in bacterial pathogenicity susceptible hosts. Xanthomonas axonopodis pv. manihotis (Xam), causal agent of cassava blight, injects several cell, including TALE1(Xam) . protein is a member Transcriptional Activator-Like (TALE) family,...
Xanthomonas oryzae (Xo) are globally important rice pathogens. Virulent lineages from Africa and Asia less virulent strains the United States have been well characterized. campestris pv. leersiae (Xcl), first described in 1957, causes bacterial streak on perennial grass, Leersia hexandra, is a close relative of Xo. L. member Poaceae, highly similar to phylogenetically, ubiquitous around paddies, reservoir pathogenic We used long read, single molecule real time (SMRT) genome sequences five...
Abstract Trans-acting small interfering RNAs (ta-siRNAs) and natural cis-antisense siRNAs (cis-nat-siRNAs) are recently discovered (sRNAs) involved in post-transcriptional gene silencing. ta-siRNAs transcribed from genomic loci require processing by microRNAs (miRNAs). cis-nat-siRNAs derived antisense produced the simultaneous transcription of overlapping genes. Their roles many plant processes, including pathogen response, mostly unknown. In this work, we employed a bioinformatic approach...
The gammaproteobacterial genus Xanthomonas contains several plant-pathogenic species. Many economically important crops serve as hosts for different spp., making this a considerable threat to global agriculture and food security. Rice-pathogenic X. oryzae pathovars (Xoo) oryzicola (Xoc) cause bacterial blight (BB) leaf streak (BLS), respectively, with major losses farmers in the tropics. Other pathogens include euvesicatoria, gardneri perforans species that infect solanaceous plants, various...
Xanthomonas vasicola pv. vasculorum is an emerging bacterial plant pathogen that causes leaf streak on corn. First described in South Africa 1949, reports of this have greatly increased the past years America and United States. The rapid spread disease North may be due to more favorable environmental conditions, susceptible hosts and/or genomic changes favored spread. To understand whether genetic mechanisms exist behind recent X. vasculorum, we used comparative genomics identify gene...
Phenotypic responses to biotic stresses are often studied as the interactions between two species; however, in phytobiome, these frequently result from complex involving several organisms. Here, we show that variation chlorosis caused by Russian wheat aphid (Diuraphis noxia) feeding is determined, part, aphid-associated bacteria. Proteomic analysis of fluids injected into a sterile medium during indicate 99% proteins bacterial origin. Of these, greatest proportion produced bacteria order...
Transcription activator-like effectors (TALEs) play a significant role for pathogenesis in several xanthomonad pathosystems. Xanthomonas phaseoli pv. manihotis (Xpm), the causal agent of Cassava Bacterial Blight (CBB), uses TALEs to manipulate host metabolism. Information about Xpm and their target genes cassava is scarce, but has been growing last few years. We aimed characterize TALE diversity Colombian strains screen TALE-targeted gene candidates. selected eighteen based on neutral...
Abstract Extracellular plant small RNAs (sRNAs) and/or double-stranded RNA (dsRNA) precursors act as triggers of RNAi in interacting filamentous pathogens 1–7 . However, whether any these extracellular species direct gene silencing plant-interacting bacteria remains unknown. Here, we show that Arabidopsis transgenic plants expressing sRNAs directed against virulence factors a Pseudomonas syringae strain, reduce its pathogenesis. This Antibacterial Gene Silencing (AGS) phenomenon is by...