A5085481030- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Legume Nitrogen Fixing Symbiosis
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant Reproductive Biology
- Biosensors and Analytical Detection
- Rice Cultivation and Yield Improvement
- Plant Virus Research Studies
- Advanced biosensing and bioanalysis techniques
- Plant Disease Resistance and Genetics
- Plant Pathogens and Fungal Diseases
- Nanopore and Nanochannel Transport Studies
- Ubiquitin and proteasome pathways
- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- Plant tissue culture and regeneration
- Bioenergy crop production and management
Fujian Agriculture and Forestry University
2024
University of Missouri
2019-2023
South China Agricultural University
2013-2022
Iowa State University
2016-2019
Abstract Bacterial blight of rice is an important disease in Asia and Africa. The pathogen, Xanthomonas oryzae pv. ( Xoo ), secretes one or more six known transcription-activator-like effectors (TALes) that bind specific promoter sequences induce, at minimum, the three host sucrose transporter genes SWEET11 , SWEET13 SWEET14 expression which required for susceptibility. We used CRISPR–Cas9-mediated genome editing to introduce mutations all SWEET gene promoters. Editing was further informed...
Abstract Plant pathogenic bacteria of the genus Xanthomonas possess transcription activator-like effectors (TALEs) that activate disease susceptibility genes in host, inducing a state disease. Here we report some isolates rice pathogen oryzae use truncated versions TALEs (which term interfering TALEs, or iTALEs) to overcome resistance. In comparison with typical iTALEs lack activation domain but retain nuclear localization motifs and are expressed from were previously considered pseudogenes....
Abstract Blight-resistant rice lines are the most effective solution for bacterial blight, caused by Xanthomonas oryzae pv. ( Xoo ). Key resistance mechanisms involve SWEET genes as susceptibility factors. Bacterial transcription activator-like (TAL) effectors bind to effector-binding elements (EBEs) in gene promoters and induce genes. EBE variants that cannot be recognized TAL abrogate induction, causing resistance. Here we describe a diagnostic kit enable analysis of blight field...
Xanthomonas oryzae pathovar (Xoo) uses transcription activator-like effectors (TALEs) to cause bacterial blight (BB) in rice. In turn, rice has evolved several mechanisms resist BB by targeting TALEs. One mechanism involves the nucleotide-binding leucine-rich repeat (NLR) resistance gene Xa1 and Reciprocally, Xoo TALE variants, C-terminally truncated versions (interfering TALEs or iTALEs), overcome resistance. However, it remains unknown what extent two co-adaptive mediate Xoo-rice...
The CRISPR/Cas9 system has become a powerful tool for targeted mutagenesis. Switchgrass (Panicum virgatum L.) is high yielding perennial grass species that been designated as model biomass crop by the U.S. Department of Energy. self-infertility and ploidy level make it difficult to study gene function or improve germplasm. To overcome these constraints, we explored feasibility using mutagenesis in tetraploid cultivar 'Alamo' switchgrass. We first developed transient assay which...
Bacterial leaf blight (BB) of rice, caused by Xanthomonas oryzae pv. (Xoo), threatens global food security and the livelihood small-scale rice producers. Analyses Xoo collections from Asia, Africa Americas demonstrated complete continental segregation, despite robust trade. Here, we report unprecedented BB outbreaks in Tanzania. The causative strains, unlike endemic African Xoo, carry Asian-type TAL effectors targeting sucrose transporter SWEET11a iTALes suppressing Xa1. Phylogenomics...
Abstract Protein activity, abundance, and stability can be regulated by post-translational modification including ubiquitination. Ubiquitination is conserved among eukaryotes plays a central role in modulating cellular function; yet, we lack comprehensive catalogs of proteins that are modified ubiquitin plants. In this study, describe an antibody-based approach to enrich ubiquitinated peptides coupled with isobaric labeling enable quantification up 18-multiplexed samples. This identified...
Summary Transcription Activator‐Like effectors (TALes) represent the largest family of type III among pathogenic bacteria and play a critical role in process infection. Strains Xanthomonas oryzae pv. (Xoo) some strains other pathogens contain large numbers TALe genes. Previous techniques to clone individual or complement genes through conventional strategies are inefficient time‐consuming due multiple (up 29 copies) given genome, technically challenging repetitive sequences 33 nearly...
Abstract TALes (Transcription Activator-Like effectors) represent the largest family of type III effectors among pathogenic bacteria and play a critical role in process infection. Strains Xanthomonas oryzae pv. (Xoo) some strains other pathogens contain large numbers TALe genes. Previous techniques to clone individual or complement genes through conventional strategies are inefficient time-consuming due multiple (up 29 copies) given genome technically challenging repetitive sequences 33...
Abstract Bacterial leaf blight (BB) of rice, caused by Xanthomonas oryzae pv. ( Xoo ), threatens global food security and the livelihood small-scale rice producers. Analyses collections from Asia, Africa Americas demonstrated surprising continental segregation, despite robust trade. Here, we report unprecedented BB outbreaks in Tanzania. The causative strains, unlike endemic , carry Asian-type TAL effectors targeting sucrose transporter SWEET11a suppressing Xa1 . Phylogenomics clustered...