A5070322730- Sugarcane Cultivation and Processing
- Plant Disease Resistance and Genetics
- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Biofuel production and bioconversion
- Plant Pathogens and Fungal Diseases
- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Legume Nitrogen Fixing Symbiosis
- Phytoplasmas and Hemiptera pathogens
- Genomics and Phylogenetic Studies
- Plant pathogens and resistance mechanisms
- Infections and bacterial resistance
- Cocoa and Sweet Potato Agronomy
- Animal Genetics and Reproduction
- Veterinary medicine and infectious diseases
- Chromosomal and Genetic Variations
- Leptospirosis research and findings
- Insect symbiosis and bacterial influences
- Plant Disease Management Techniques
- Evolution and Paleontology Studies
- Yeasts and Rust Fungi Studies
- Plant Pathogens and Resistance
- Plant Gene Expression Analysis
- CRISPR and Genetic Engineering
Universidade de São Paulo
2015-2024
Secretaria de Agricultura e Abastecimento
2002-2021
Instituto Butantan
2018
University of California, Davis
2018
Universidade Cidade de São Paulo
2014
Michigan State University
1993-2009
Universidade Federal do ABC
2008
Laboratório Nacional de Computação Científica
2005-2008
Instituto de Botânica
2002-2008
Universidade Brasil
2004
Leptospira species colonize a significant proportion of rodent populations worldwide and produce life-threatening infections in accidental hosts, including humans. Complete genome sequencing interrogans serovar Copenhageni comparative analysis with the available Lai reveal that despite overall genetic similarity there are structural differences, large chromosomal inversion extensive variation number distribution insertion sequence elements. Genome elucidates many novel aspects leptospiral...
Xylella fastidiosa is a xylem-dwelling, insect-transmitted, gamma-proteobacterium that causes diseases in many plants, including grapevine, citrus, periwinkle, almond, oleander, and coffee. X. has an unusually broad host range, extensive geographical distribution throughout the American continent, induces diverse disease phenotypes. Previous molecular analyses indicated three distinct groups of isolates were expected to be genetically divergent. Here we report genome sequence (Temecula...
We report novel features of the genome sequence Leptospira interrogans serovar Copenhageni, a highly invasive spirochete. species colonize significant proportion rodent populations worldwide and produce life-threatening infections in mammals. Genomic analysis reveals presence competent transport system with 13 families genes encoding for major transporters including three-member component efflux compatible long-term survival this organism. The leptospiral contains broad array regulatory...
Coffee is one of the most valuable agricultural commodities and ranks second on international trade exchanges. The genus Coffea belongs to Rubiaceae family which includes other important plants. contains about 100 species but commercial production based only two species, arabica canephora that represent 70 % 30 total coffee market, respectively. Brazilian Genome Project was designed with objective making modern genomics resources available scientific community, working different aspects...
Sugarcane is the source of sugar in all tropical and subtropical countries becoming increasingly important for bio-based fuels. However, its large (10 Gb), polyploid, complex genome has hindered based breeding efforts. Here we release largest most diverse set sugarcane sequences to date, as part an on-going initiative provide a genomic information resource, with ultimate goal producing gold standard genome. Three hundred seventeen chiefly euchromatic BACs were sequenced. A reference one...
Sporisorium scitamineum is a biotrophic fungus responsible for the sugarcane smut, worldwide spread disease. This study provides complete sequence of individual chromosomes S. from telomere to achieved by combination PacBio long reads and Illumina short data, as well draft second fungal strain. Comparative analysis previous available sequences another strain detected few polymorphisms among three genomes. The novel described herein allowed us identify annotate extended subtelomeric regions,...
The genome sequence of Leifsonia xyli subsp. xyli, which causes ratoon stunting disease and affects sugarcane worldwide, was determined. single circular chromosome CTCB07 2.6 Mb in length with a GC content 68% 2,044 predicted open reading frames. analysis also revealed 307 pseudogenes, is more than any bacterial plant pathogen sequenced to date. Many these if functional, would likely be involved the degradation heteropolysaccharides, uptake free sugars, synthesis amino acids. Although L. has...
Sugarcane smut disease is caused by the biotrophic fungus Sporisorium scitamineum. The characterized development of a whip-like structure from primary meristems, where billions teliospores are produced. also causes tillering and low sucrose high fiber contents, reducing cane productivity. We investigated biological events contributing to symptoms in intermediate-resistant sugarcane genotype examining transcriptional profiles (RNAseq) shortly after inoculating plants immediately whip...
Background and Aims Sugarcane smut is caused by the fungus Sporisorium scitamineum (Ustilaginales/Ustilaginomycotina/Basidiomycota), which responsible for losses in sugarcane production worldwide. Infected plants show a profound metabolic modification resulting development of whip-shaped structure (sorus) composed mixture plant tissues fungal hyphae. Within this structure, ustilospores develop disseminate disease. Despite importance disease, detailed histopathological analysis plant–pathogen...
Sugarcane smut disease, caused by the biotrophic fungus Sporisorium scitamineum, is characterized development of a whip-like structure from plant meristem. The disease causes negative effects on sucrose accumulation, fiber content and juice quality. aim this study was to exam whether transcriptomic changes already described during infection sugarcane S. scitamineum result in at metabolomic level. To address question, an analysis conducted initial stage interaction through progression...
Abstract Passiflora edulis is the major species of passionflowers grown worldwide, mainly for juice production and fresh fruit, in climates ranging from cool subtropical (purple variety) to warm tropical (yellow variety). The bacterial leaf spot, caused by Xanthomonas axonopodis pv. passiflorae ( Xap ) can be a serious disease affecting passion fruit commercial orchards, particularly under moist field conditions. In this study we describe first analysis host gene expression pathosystem. We...
Host specialization is a key evolutionary process for the diversification and emergence of new pathogens. However, molecular determinants host range are poorly understood. Smut fungi biotrophic pathogens that have distinct narrow ranges based on largely unknown genetic determinants. Hence, we aimed to expand comparative genomics analyses smut by including more species infecting different hosts define orphans positively selected genes gain further insights into genetics basis specialization....
Traditional sugarcane cultivars (Saccharum officinarum) proved highly susceptible to diseases, and this led breeders progress interspecific crosses resulting in disease resistance. A backcrossing program S. officinarum was then required boost sucrose content. Clonal selection across generations incorporation of other germplasm into cultivated backgrounds established the (narrow) genetic base modern spp.), which have a man-made genome. The genome complexity has inspired several molecular...
Abstract Background Resistance genes composing the two-layer immune system of plants are thought as important markers for breeding pathogen-resistant crops. Many have been attempts to establish relationships between genomic content Gene Analogs (RGAs) modern sugarcane cultivars its degrees resistance diseases such smut. However, due highly polyploid and heterozygous nature genome, large scale RGA predictions is challenging. Results We predicted, searched orthologs, investigated features RGAs...
To rapidly and cost-effectively generate gene expression data, we developed an annotated unigene database of common bean (Phaseolus vulgaris L.). In this study, 3 cDNA libraries were constructed from the breeding line SEL1308, 1 young leaf 2 seedlings inoculated or not with fungal pathogen Colletotrichum lindemuthianum (Sacc. & Magnus) Briosi Cavara, which causes anthracnose in bean. date, 5255 single-pass sequences have been included after selection based on sequence quality. These ESTs...
Despite of the importance ratoon stunting disease, little is known on responses sugarcane to its causal agent, vascular bacterial endophyte Leifsonia xyli subsp. xyli. The transcriptome and proteome young plants a susceptible cultivar with no symptoms but relative low high titers were compared at 30 60 days after inoculation. Increased associated alterations in expression 267 cDNAs abundance 150 proteins involved plant growth, hormone metabolism, signal transduction defense responses. Some...
Alternative splicing (AS) promotes transcriptome and proteome diversity during growth, development, stress responses in eukaryotes. Genome-wide studies of AS sugarcane (Saccharum spp.) are lacking, mainly due to the absence a high-quality sequenced reference genome, sugarcane's large, complex variable chromosome numbers polyploidy cultivars. Here, we analyzed changes isoform-level landscape infection with smut fungus (Sporisorium scitamineum) using hybrid approach involving Sorghum bicolor...