A5079193460- Plant Pathogenic Bacteria Studies
- Legume Nitrogen Fixing Symbiosis
- Plant-Microbe Interactions and Immunity
- Cardiomyopathy and Myosin Studies
- Vibrio bacteria research studies
- Genomics and Phylogenetic Studies
- Bacterial biofilms and quorum sensing
- Photosynthetic Processes and Mechanisms
- RNA and protein synthesis mechanisms
- Muscle Physiology and Disorders
- Leptospirosis research and findings
- Bacterial Genetics and Biotechnology
- Enzyme Production and Characterization
- Bacteriophages and microbial interactions
- RNA Research and Splicing
- SARS-CoV-2 and COVID-19 Research
- Enzyme Structure and Function
- Microbial Metabolic Engineering and Bioproduction
- Escherichia coli research studies
- Plant Parasitism and Resistance
- Infections and bacterial resistance
- Advanced biosensing and bioanalysis techniques
- Antibiotic Resistance in Bacteria
- Insect Resistance and Genetics
- COVID-19 Clinical Research Studies
Institute of Chemistry
2025
Universidade de São Paulo
2015-2024
Instituto Federal Goiano
2021
Universidade Federal de Minas Gerais
2021
La Trobe University
2013
Instituto de Botânica
2007
SUPERA Park of Innovation and Technology of Ribeirão Preto
2007
Universidade Brasil
2006
Universidade Federal de São Paulo
2000
University of Alberta
1997
Type IV secretion systems (T4SSs) are multiprotein complexes that transport effector proteins and protein–DNA through bacterial membranes to the extracellular milieu or directly into cytoplasm of other cells. Many bacteria family Xanthomonadaceae, which occupy diverse environmental niches, carry a T4SS with unknown function but several characteristics distinguishes it from T4SSs. Here we show Xanthomonas citri provides these cells capacity kill Gram-negative species in contact-dependent...
Calcium binding to regulatory sites located in the NH2-terminal domain of troponin C (TnC) induces a conformational change that blocks inhibitory action I (TnI) and triggers muscle contraction. We used deletion mutants TnI conjunction with series TnC understand structural functional relationship between different regions domains. Our results indicate is organized into which interact an antiparallel fashion corresponding TnC. Functional studies show COOH-terminal region TnI, when linked...
RpfG is a paradigm for class of widespread bacterial two-component regulators with CheY-like receiver domain attached to histidine-aspartic acid-glycine-tyrosine-proline (HD-GYP) cyclic di-GMP phosphodiesterase domain. In the plant pathogen Xanthomonas campestris pv. ( Xcc ), system comprising and complex sensor kinase RpfC implicated in sensing responding diffusible signaling factor (DSF), which essential cell–cell signaling. RpfF involved synthesizing DSF, mutations rpfF , rpfG or rpfC...
Bacterial type IV secretion systems (T4SS) are a highly diversified but evolutionarily related family of macromolecule transporters that can secrete proteins and DNA into the extracellular medium or target cells. It was recently shown subtype T4SS harboured by plant pathogen Xanthomonas citri transfers toxins Here, we show similar from multi-drug-resistant opportunistic Stenotrophomonas maltophilia is proficient in killing competitor bacterial species. T4SS-dependent duelling between S. X....
Bacteria use extracellular levels of small diffusible autoinducers to estimate local cell-density (quorum-sensing) and regulate complex physiological processes. The quorum-sensing signal transduction pathway Xanthomonas spp. phytopathogens has special features that distinguish it from other pathogens. This consists RpfF, necessary for the production unique autoinducer 'diffusible signalling factor' (DSF), RpfC RpfG, a two-component system DSF-dependent pathogenicity factors cellular...
The PilZ protein was originally identified as necessary for type IV pilus (T4P) biogenesis. Since then, a large and diverse family of bacterial homology domains have been identified, some which implicated in signaling pathways that control important processes, including motility, virulence biofilm formation. Furthermore, many domains, though not itself, shown to bind the second messenger bis(3′→5′)cyclic diGMP (c-diGMP). crystal structures orthologs from Xanthomonas axonopodis pv citri...
The RsmA/CsrA family of the post-transcriptional regulators bacteria is involved in regulation many cellular processes, including pathogenesis. In this study, we demonstrated that rsmA not only required for full virulence phytopathogenic bacterium Xanthomonas citri subsp. (XCC) but also contributes to triggering hypersensitive response (HR) non-host plants. Deletion resulted significantly reduced host plant sweet orange and a delayed weakened HR Nicotiana benthamiana. Microarray,...
The contraction of skeletal muscle is regulated by Ca2+ binding to troponin C, which results in an internal reorganization the interactions within troponin-tropomyosin complex. Troponin T necessary for Ca2+-dependent inhibition and activation actomyosin. consists extended NH2-terminal domain that interacts with tropomyosin a globular COOH-terminal tropomyosin, I, C. In this study we used recombinant I fragments delimit further structural regulatory thin filament. Our show following: (i)...
The recently sequenced genome of the bacterial plant pathogen Xanthomonas axonopodis pv. citri contains two virB gene clusters, one on chromosome and a 64-kb plasmid, each which codes for previously uncharacterized type IV secretion system (T4SS). Here we used yeast two-hybrid assay to identify protein-protein interactions in these systems. Our results revealed between known T4SS components as well involving hypothetical proteins coded by open reading frames X. loci. indicate that both loci...
Type IV secretion systems (T4SS) are used by Gram-negative bacteria to translocate protein and DNA substrates across the cell envelope into target cells. Translocation outer membrane is achieved via a ringed tetradecameric complex made up of small VirB7 lipoprotein (normally 30 45 residues in mature form) C-terminal domains VirB9 VirB10 subunits. Several species from genera Xanthomonas phytopathogens possess an uncharacterized type system with some distinguishing features, one which...
Signal transduction pathways mediated by cyclic-bis(3'→5')-dimeric GMP (c-di-GMP) control many important and complex behaviors in bacteria. C-di-GMP is synthesized through the action of GGDEF domains that possess diguanylate cyclase activity degraded EAL or HD-GYP with phosphodiesterase activity. There mounting evidence some c-di-GMP-mediated require protein-protein interactions between members GGDEF, EAL, PilZ protein domain families. For example, have been observed from FimX Xanthomonas...
Bacterial type IV pili (T4P) are long, flexible surface filaments that consist of helical polymers mostly pilin subunits. Cycles polymerization, attachment, and depolymerization mediate several pilus-dependent bacterial behaviors, including twitching motility, adhesion, pathogenicity, natural transformation, escape from immune system defense mechanisms, biofilm formation. The Xanthomonas citri subsp. strain 306 genome codes for a large set genes involved in T4P biogenesis regulation includes...
Summary Plant‐associated bacteria of the genus Xanthomonas cause disease in a wide range economically important crops. However, their ability to persist environment is still poorly understood. Predation by amoebas represents major selective pressure bacterial populations environment. In this study, we show that X. citri type 6 secretion system (T6SS) promotes resistance predation soil amoeba Dictyostelium discoideum . We found an extracytoplasmic function (ECF) sigma factor (EcfK) required...
The type VI secretion system (T6SS) secretes antibacterial effectors into target competitors. Salmonella spp. encode five phylogenetically distinct T6SSs. Here, we characterize the function of SPI-22 T6SS bongori showing that it has activity and identify a group (TseV1–4) containing an N-terminal PAAR-like domain C-terminal VRR-Nuc encoded next to cognate immunity proteins with DUF3396 (TsiV1–4). TseV2 TseV3 are toxic when expressed in Escherichia coli bacterial competition assays confirm...
Xanthomonas axonopodis pv. citri utilizes the type III effector protein PthA to modulate host transcription promote citrus canker. proteins belong AvrBs3/PthA family and carry a domain comprising tandem repeats of 34 amino acids that mediates protein-protein protein-DNA interactions. We show here variants PthAs from single bacterial strain localize nucleus plant cells form homo- heterodimers through association their repeat regions. hypothesize might also interact with distinct targets....
Herein, we present a novel esterase enzyme, Ade1, isolated from metagenomic library of Amazonian dark earths soils, demonstrating its broad substrate promiscuity by hydrolyzing ester bonds linked to aliphatic groups. The three-dimensional structure the enzyme was solved in presence and absence (tributyrin), revealing classification within α/β-hydrolase superfamily. Despite being monomeric enzymatic assays reveal cooperative behavior with sigmoidal profile (initial velocities vs...