A5062533452- Insect Resistance and Genetics
- Genetically Modified Organisms Research
- Entomopathogenic Microorganisms in Pest Control
- Insect and Pesticide Research
- Insect Pest Control Strategies
- Phytoplasmas and Hemiptera pathogens
- Cocoa and Sweet Potato Agronomy
- Plant Pathogenic Bacteria Studies
- Genomics and Phylogenetic Studies
- CRISPR and Genetic Engineering
- Bacillus and Francisella bacterial research
- Salmonella and Campylobacter epidemiology
- Legume Nitrogen Fixing Symbiosis
- Insect symbiosis and bacterial influences
- Microbial Community Ecology and Physiology
- Bacteriophages and microbial interactions
- Banana Cultivation and Research
- Plant-Microbe Interactions and Immunity
- Genetic diversity and population structure
- Plant nutrient uptake and metabolism
- Viral Infectious Diseases and Gene Expression in Insects
- Infections and bacterial resistance
- Bacterial biofilms and quorum sensing
- Microbial Metabolism and Applications
- Insect Utilization and Effects
Universidade Estadual Paulista (Unesp)
2013-2024
Instituto de Tecnologia e Pesquisa
2024
Núcleo de Pesquisas Aplicadas (Brazil)
2000-2019
Fundação de Apoio à Universidade Federal de São Paulo
2015
University of Nevada, Reno
2008
Universidade Estadual de Londrina
2005
Universidade Federal da Bahia
2005
Universidade de São Paulo
2005
Universidade Estadual de Campinas (UNICAMP)
2004
Universidade Federal do Rio de Janeiro
2004
Leptospira species colonize a significant proportion of rodent populations worldwide and produce life-threatening infections in accidental hosts, including humans. Complete genome sequencing interrogans serovar Copenhageni comparative analysis with the available Lai reveal that despite overall genetic similarity there are structural differences, large chromosomal inversion extensive variation number distribution insertion sequence elements. Genome elucidates many novel aspects leptospiral...
Xylella fastidiosa is a xylem-dwelling, insect-transmitted, gamma-proteobacterium that causes diseases in many plants, including grapevine, citrus, periwinkle, almond, oleander, and coffee. X. has an unusually broad host range, extensive geographical distribution throughout the American continent, induces diverse disease phenotypes. Previous molecular analyses indicated three distinct groups of isolates were expected to be genetically divergent. Here we report genome sequence (Temecula...
Coffee is one of the most valuable agricultural commodities and ranks second on international trade exchanges. The genus Coffea belongs to Rubiaceae family which includes other important plants. contains about 100 species but commercial production based only two species, arabica canephora that represent 70 % 30 total coffee market, respectively. Brazilian Genome Project was designed with objective making modern genomics resources available scientific community, working different aspects...
The genome sequence of Leifsonia xyli subsp. xyli, which causes ratoon stunting disease and affects sugarcane worldwide, was determined. single circular chromosome CTCB07 2.6 Mb in length with a GC content 68% 2,044 predicted open reading frames. analysis also revealed 307 pseudogenes, is more than any bacterial plant pathogen sequenced to date. Many these if functional, would likely be involved the degradation heteropolysaccharides, uptake free sugars, synthesis amino acids. Although L. has...
The polyphagous pests belonging to the genus Spodoptera are considered be among most important causes of damage and widely distributed throughout Americas'. Due extensive use genetically modified plants containing Bacillus thuringiensis genes that code for insecticidal proteins, resistant insects may arise. To prevent development resistance, pyramided plants, which express multiple proteins act through distinct mode actions, can used. This study analyzed mechanisms action Cry1Ia10 Vip3Aa on...
Rhizobium tropici, a member of the Rhizobiaceae family, has ability to synthesize and secrete extracellular polysaccharides (EPS). Rhizobial EPS have attracted much attention from scientific industrial communities. isolates R. tropici mutants that produced higher levels than wild-type strain SEMIA4080 were used in present study. The results suggested heteropolymer structure for these composed by glucose galactose as prevailing monomer unit. All samples exhibited typical non-Newtonian...
ABSTRACT Little is known about genetic exchanges in natural populations of bacteria the spore-forming Bacillus cereus group, because no population genetics studies have been performed with local sympatric populations. We isolated strains thuringiensis and B. from small samples soil collected at same time two separate geographical sites, one within forest other edge forest. A total 100 98 were characterized by electrophoresis to determine allelic composition nine enzymatic loci. observed...
Second generation Bt crops (insect resistant carrying Bacillus thuringiensis genes) combine more than one gene that codes for insecticidal proteins in the same plant to provide better control of agricultural pests. Some new combinations involve co-expression cry and vip genes. Because Cry Vip have different midgut targets possibly mechanisms toxicity, it is important evaluate possible synergistic or antagonistic interactions between these two classes toxins. Three members Cry1 class three...
Exopolysaccharide (EPS) are produced by a diverse of rhizobia species and has been demonstrated to be bioemulsifier with potential applications in the degradation hydrocarbons. In present study, attempts were made obtain new exopolysaccharide production Rhizobium tropici (SEMIA 4080 MUTZC3) strains during growth on hydrocarbon substrate. Under different cultivation conditions, high molecular weight exopolysaccharides from cultivated for 96h mainly consisted carbohydrates (79-85%) low...
Two hundred and eighteen Bacillus thuringiensis isolates from Brazil were characterized by the presence of crystal protein genes PCR with primers specific to different cry cyt genes. Among these isolates, 95 selected according their geographic origin for genetic characterization 16S rRNA gene, RAPD, plasmid profile. Isolates containing cry1 most abundant (48%) followed cry11 (7%) cry8 (2%). Finally, 40.3% did not produce any product. The profile RAPD analysis showed a remarkable diversity...
A novel combination of structurally simple, high-rate horizontal anaerobic reactors installed in series was used to treat swine wastewater. The maintained stable pH, alkalinity, and volatile acid levels. Removed chemical oxygen demand (COD) represented 68% the total, average specific methane production 0.30 L CH4 (g removed CODtot)−1. In addition, next-generation sequencing quantitative real-time PCR analyses were explore methane-producing Archaea microbial diversity. At least 94% sludge...
Abstract Over 40,000 sugarcane (Saccharum officinarum) consensus sequences assembled from 237,954 expressed sequence tags were compared with the protein and DNA other angiosperms, including genomes of Arabidopsis rice (Oryza sativa). Approximately two-thirds transcriptome have similar in Arabidopsis. These may represent a core set proteins or domains that are conserved among monocots eudicots probably encode for essential angiosperm functions. The remaining putative monocot-specific genetic...
Assimilation of nitrate and ammonium are vital procedures for plant development growth. From these primary paths inorganic nitrogen assimilation, this metabolism integrates diverse biosynthesis macromolecules, such as amino acids nucleotides, the central intermediate metabolism, like carbon photorespiration. This paper reports research performed in CitEST (Citrus Expressed Sequence Tag) database main genes involved those previously described other organisms. The results show that a complete...
Aedes aegypti (L.), the main vector of dengue fever in Brazil, has been controlled with use massive chemical products, contributing to development resistance and decreasing insect control efficiency. The dipterans bioinsecticides based on Bacillus thuringiensis satisfactory, due production insecticidal proteins denominated Cry (crystal), Cyt (cytolytic) toxins Chi (chitinase), synergistic effects among them. present work aimed select B. isolates efficient against A. larvae. A bacterial...
Thirty nine isolates of Flavobacterium columnare from Brazilian fish farms had their carbohydrate composition EPS evaluated by high efficiency liquid chromatography, using the phenol-sulfuric acid method EPS. The occurrence capsules on F. cells was not directly related to biofilm formation, and predominant monosaccharide is glucose.
The biological potential of Vip and Cry proteins from Bacillus is well known widely established. Thus, it important to look for new genes showing different modes action, selecting those with differentiated entomotoxic activity against Diatraea flavipennella Elasmopalpus lignosellus , which are secondary pests sugarcane. Therefore, Cry1 Vip3 were expressed in Escherichia coli their toxicities evaluated based on bioassays using neonate larvae. Of those, the most toxic Cry1Ac Vip3Aa considering...
O objetivo deste trabalho foi avaliar a suscetibilidade das lagartas Anticarsia gemmatalis (Lepidoptera: Erebidae) e Chrysodeixis includens Noctuidae) às proteínas Cry1 Vip3A, bem como determinar se há interação entre essas no controle duas espécies. Bioensaios com as isoladas em combinações foram realizados, concentrações letais CL50 CL90 estimadas para cada condição. As Cry1Aa, Cry1Ac Vip3Af mais efetivas de A. gemmatalis, enquanto Cry1Ac, Vip3Aa C. includens. Cry1Ca causaram maior...
The giant anteater (Myrmecophaga tridactyla) is found from Belize and Guatemala to Paraguay Argentina. Its conservation status considered vulnerable by IUCN. Here we report the isolation characterization of six microsatellite loci. Positive loci for (GT)n were isolated using a magnetic bead hybridization selection protocol. number alleles per locus as well heterozygosity PCR conditions are described. These will be useful studying population structure, genetic diversity, paternity in M....
S. Pullorum (SP) and Gallinarum (SG) are very similar. They the agents of pullorum disease fowl typhoid, respectively, two diseases responsible for economic losses in poultry production. Although SP SG difficult to be differentiated routine laboratory procedures, ability metabolize ornithine is a biochemical test that may used achieve this aim. While able decarboxylate amino acid, not. However, isolation strains showing atypical behavior has made differentiation difficult. One genes...
ABSTRACT: Cockroach control is performed by the application of chemical insecticides which exert high selective pressure on populations and introduces synthetic substances in environment, motivating search for other methods such as entomopathogenic fungi. The objectives this study were to investigate pathogenicity JAB 42 Aspergillus westerdijkiae females oothecae Periplaneta americana demonstrate its mechanism action oothecae. Suspensions containing 106 108 conidia/ml used infect Mortality...
O objetivo deste trabalho foi caracterizar o gene vip3A de Bacillus thuringiensis e verificar a toxicidade da proteína Vip3Aa50 larvas lagarta-do-cartucho (Spodoptera frugiperda) lagarta-da-soja (Anticarsia gemmatalis). amplificado por PCR, com iniciadores específicos, gerou um fragmento 2.370 pb. Esse clonado em vetor pGEM-T Easy e, seguida, sequenciado, subclonado expressão pET-28a (+) inserido células Escherichia coli BL21 (DE3). A induzida isopropil-β-D-1-tiogalactopiranosídeo (IPTG),...