A5072052720- Phytoplasmas and Hemiptera pathogens
- Cocoa and Sweet Potato Agronomy
- Plant Pathogenic Bacteria Studies
- Banana Cultivation and Research
- Insect symbiosis and bacterial influences
- Insect-Plant Interactions and Control
- Plant Pathogens and Fungal Diseases
- Plant Virus Research Studies
- Entomopathogenic Microorganisms in Pest Control
- Research on scale insects
- Plant-Microbe Interactions and Immunity
- Mosquito-borne diseases and control
- Sugarcane Cultivation and Processing
- Pineapple and bromelain studies
- Plant and animal studies
- Mycorrhizal Fungi and Plant Interactions
- Plant Physiology and Cultivation Studies
- Infections and bacterial resistance
- Horticultural and Viticultural Research
- Plant pathogens and resistance mechanisms
- Dental Implant Techniques and Outcomes
- Dental Research and COVID-19
- Plant tissue culture and regeneration
- Helminth infection and control
- Insect Resistance and Genetics
Fundo de Defesa da Citricultura
2012-2024
Universidade Estadual Paulista (Unesp)
2000-2022
Chongqing University
2014
Universidade de Ribeirão Preto
2000-2003
Universidade de Mogi das Cruzes
2003
Universidade Estadual de Campinas (UNICAMP)
2003
Agronomical Institute of Campinas
2003
Brazilian Agricultural Research Corporation
2003
Louisiana State University Agricultural Center
1996-2001
The biology and behaviour of the psyllid Diaphorina citri Kuwayama (Hemiptera: Sternorrhyncha: Liviidae), major insect vector bacteria associated with huanglongbing, have been extensively studied respect to host preferences, thermal requirements, responses visual chemical volatile stimuli. However, development in relation ontogeny immature citrus flush growth has not clearly defined or illustrated. Such information is important for determining timing frequency measures used minimize...
Huanglongbing (HLB) is the most destructive, yet incurable disease of citrus. Finding sources genetic resistance to HLB-associated ‘ Candidatus Liberibacter asiaticus’ (Las) becomes strategic warrant crop sustainability, but no resistant Citrus genotypes exist. Some relatives family Rutaceae, subfamily Aurantioideae, were described as full-resistant Las, they are phylogenetically far, thus incompatible with . Partial was indicated for certain cross-compatible types. Moreover, other from...
In São Paulo State, Brazil, 'Candidatus Liberibacter americanus' and asiaticus' are associated with huanglongbing (HLB). Affected municipalities occur mainly in the central southern regions, where annual number of hours above 30°C is two to five times lower than that extreme northern western regions. The influence temperature on sweet orange trees infected 'Ca. L. or was studied temperature-controlled growth chambers. Symptom progression new shoots naturally experimentally graft-inoculated...
Xylella fastidiosa is a vector-borne, plant-pathogenic bacterium that causes disease in citrus (citrus variegated chlorosis [CVC]) and coffee (coffee leaf scorch [CLS]) plants Brazil. CVC CLS occur sympatrically share leafhopper vectors; thus, determining whether X. isolates can be dispersed from one crop to another cause of epidemiological importance. We sought clarify the genetic biological relationships between CVC- CLS-causing isolates. used cross-inoculation bioassays microsatellite...
In Brazil 'Candidatus Liberibacter asiaticus' and 'Ca. L. americanus' cause huanglongbing (also known as greening), the most destructive citrus disease. A shift in pathogen prevalence was observed over time, with a disproportional increase occurrence. Graft transmission experiments were used for comparative study of both species using budsticks from symptomatic branches field-affected trees inoculum. The plants inoculated or alone, simultaneously species. Symptom manifestation conventional...
Genetically distinct strains of the plant bacterium Xylella fastidiosa ( Xf ) are responsible for a variety diseases, accounting severe economic damage throughout world. Using as reference genome 9a5c strain, associated with citrus variegated chlorosis (CVC), we developed microarray-based comparison involving 12 isolates, providing thorough assessment variation in genomic composition across group. Our results demonstrate that displays one largest flexible gene pools characterized to date,...
The antigenic components of excretory-secretory products (ESP) adult worms Fasciola hepatica were revealed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot analysis using sera from 20 patients infected with F. hepatica. Sera 184 other parasitic infections healthy volunteers also analyzed. It was found that the ESP composed more than 11 polypeptides; five detected in fascioliasis had molecular weights 12.4, 16.4, 19.4, 25, 27 kilodaltons (kD). Only 25- 27-kD...
Little is known about 'Candidatus Liberibacter americanus', a causal agent of huanglongbing or greening disease in Brazil, its interaction with citrus trees. Greenhouse experiments were conducted the objective determining conditions favorable for transmission from field affected trees to young potted plants, evaluate reaction multiple species disease, and determine efficiency pathogen propagation individual buds. Single buds bark pieces various sizes plus wood (budstick) that removed...
Instituto Agronomico do Parana - IAPAR, 86001-970, Londrina, PR, BrasilAutor para correspondencia: Jose Belasque Junior, e-mail: belasque@fundecitrus.com.br1. IntroducaoHuanglongbing (HLB), ou Greening, e a doenca mais importante destrutiva da citricultura mundial (Bove, 2006; Gottwald et al., 2007a). Presente de forma endemica nos continentes asiatico africano ha varias decadas, essa foi recentemente detectada dois principais paises produtores citros, Brasil Estados Unidos (Colleta Filho...
Two surveys (2005/2006 and 2009) were conducted in the state of São Paulo, Brazil, to investigate incidence ‘ Candidatus Liberibacter asiaticus’ Ca. L. americanus’, two liberibacters associated with citrus huanglongbing (HLB) disease both transmitted by Diaphorina citri , orange jasmine ( Murraya exotica ), a widespread ornamental tree cities villages. The graft‐transmissibility species, their DNA relatedness citrus‐associated liberibacters, also investigated. Quantitative PCR was applied...
'Candidatus Liberibacter asiaticus' is the most prevalent sp. associated with huanglongbing (HLB) in Brazil. Within São Paulo state (SP), HLB has spread more rapidly to and reached higher incidence regions relatively mild (cooler) summer temperatures. This suggests that climate can influence disease severity. 'Ca. L. titers on soft, immature leaves from infected 'Valencia' sweet orange plants exposed different temperature regimes adult Diaphorina citri fed for 48 h these acquisition were...
The objectives of this work were (i) to determine the influence temperature on infection citrus by ‘ Candidatus Liberibacter asiaticus’ and americanus’, two bacterial species associated with huanglongbing (HLB) in Brazil, (ii) colonization Ca. L. asiaticus’, which has taken over from americanus’ as predominant Brazil since 2008. Two experiments carried out graft‐inoculated Valencia oranges Rangpur lime rootstocks. Immediately after inoculation plants maintained for 423 days growth chambers...
Huanglongbing ( HLB ) disease is seriously threatening and/or damaging the citrus industry worldwide. Accurate detection of three species associated with disease, ‘ C andidatus Liberibacter asiaticus’, africanus’ and americanus’, essential for preventive control disease. Real‐time PCR a useful tool bacterial detection. However, nucleic acid purification steps limit number samples that can be processed by . Universal Liberibacter’ was achieved direct tissue‐printing spotting plant leaf...
Murraya paniculata and Swinglea glutinosa are aurantioid hosts of the Asian citrus psyllid (ACP) Diaphorina citri, principal vector ‘Candidatus Liberibacter asiaticus’ (Las). Las is pathogen associated with huanglongbing (HLB), form which most devastating disease Citrus species cultivars (Rutaceae: Aurantioideae). M. a common ornamental S. grown as an ornamental, rootstock, hedgerow fence plant. Because uncertain status these plants reservoirs Las, series cross-inoculation bioassays were...
The major citrus area of Brazil occupies near 450,000 ha between the Triângulo Mineiro (TM) region Minas Gerais State and south São Paulo (SPS). Significant climatic variation occurs regions which could affect huanglongbing (HLB) progress, is lower in TM. To investigate this possibility, young sweet orange shoots were sampled periodically over 2 years to determine 'Candidatus Liberibacter asiaticus' titers naturally infected trees orchards Analândia, central SPS, Frutal Comendador Gomes,...
‘Candidatus Liberibacter asiaticus’ (Las) is an unculturable, phloem-limited, insect-transmitted bacterium associated with the Asiatic form of huanglongbing (HLB), most destructive citrus disease. In Asia and Americas, it transmitted by Asian psyllid (Diaphorina citri Kuwavama). Despite considerable research, little known about processes involved in plant infection colonization Las. This study was conducted to determine whether basal portion (below girdling) important route for Las move...
In Brazil, Xylella fastidiosa is present in citrus (Citrus sinensis), coffee (Coffea arabica), and plum (Prunus sp.) crops, causing the variegated chlorosis (CVC), leaf scorch (CLS), scald (PLS). Also these crops infesting weeds, which ultimately could serve as sources of inoculum for cultivated trees, are diverse populations xylem-feeding leafhopper vectors. order to assess host range X. among weeds better understand their role epidemics, field surveys, mechanical inoculations, insect...
Greening disease of citrus is a serious known in South Africa since the late 1920s. In Africa, it associated with infection by ‘Candidatus Liberibacter africanus’, heat sensitive, phloem-limited, noncultured alpha-proteobacterium. Huanglongbing (HLB), similar, but more devastating that was described initially from China which now occurs several producing countries, different species, ‘Ca. L. asiaticus’. A africanus’ subspecies, africanus subsp. capensis’, has been found only infecting an...