A5076243476- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Phytoplasmas and Hemiptera pathogens
- Cocoa and Sweet Potato Agronomy
- Plant Virus Research Studies
- Legume Nitrogen Fixing Symbiosis
- Bacteriophages and microbial interactions
- Plant Pathogens and Fungal Diseases
- Genomics and Phylogenetic Studies
- Photosynthetic Processes and Mechanisms
- Plant Reproductive Biology
- Identification and Quantification in Food
- Insect symbiosis and bacterial influences
- Plant Disease Resistance and Genetics
- Plant Molecular Biology Research
- Aquatic Ecosystems and Phytoplankton Dynamics
- Genetic diversity and population structure
- Plant tissue culture and regeneration
- Fish Ecology and Management Studies
- Bacterial biofilms and quorum sensing
- Fish Biology and Ecology Studies
- Microbial infections and disease research
- Fish biology, ecology, and behavior
- Plant Stress Responses and Tolerance
- Environmental DNA in Biodiversity Studies
U.S. National Arboretum
2014-2025
Xuzhou Medical College
2024-2025
Agricultural Research Service
2016-2025
United States Department of Agriculture
2014-2024
Sun Yat-sen University
2023-2024
University of Toronto
2024
Jiangxi Normal University
2024
Hospital of Stomatology, Sun Yat-sen University
2023-2024
Hubei University of Medicine
2024
Jinan University
2017-2023
Abstract The cytoplasmic male sterility (CMS) phenotype in plants can be reversed by the action of nuclear-encoded fertility restorer (Rf) genes. molecular mechanism involved Rf gene–mediated processing CMS-associated transcripts is unclear, as are identities other proteins that may CMS–Rf interaction. In this study, we cloned gene Rf5 for Hong-Lian CMS rice and studied its restoration with respect to transcript atp6-orfH79. RF5, a pentatricopeptide repeat (PPR) protein, was unable bind...
An 8× draft genome was obtained and annotated for Ralstonia solanacearum race 3 biovar 2 (R3B2) strain UW551, a United States Department of Agriculture Select Agent isolated from geranium. The UW551 consisted 80,169 reads resulting in 582 contigs containing 5,925,491 base pairs, with an average 64.5% GC content. Annotation revealed predicted 4,454 protein coding open reading frames (ORFs), 43 tRNAs, 5 rRNAs; 2,793 (or 62%) the ORFs had functional assignment. compared published R. 1 tropical...
Chlorophyll-a (Chl a) is an important indicator of algal biomass frequently used to evaluate the severity cultural eutrophication. Identifying key covariates Chl a concentrations essential understand mechanisms that drive eutrophication and develop forecasting tools guide restoration process. In this study, we present novel ensemble modeling framework founded upon complementary features Random Forest (RF) Generalized Additive (GAMs). A series RF models are first developed forecast based on...
Ralstonia solanacearum, which causes bacterial wilt disease of many plant species, produces several extracellular cell wall-degrading enzymes that are suspected virulence factors. These include a previously described endopolygalacturonase (PG), PehA, and two exo-PGs. A gene encoding one the exo-PGs, pehB, was cloned from R. solanacearum K60. The DNA fragment specifying PehB contained 2,103-bp open reading frame encodes protein 74.2 kDa with typical N-terminal signal sequence. pehB product...
Odontoblastic differentiation of human stem cells from the apical papilla (hSCAPs) is crucial for continued root development and dentin formation in immature teeth with periodontitis (AP). Fat mass obesity-associated protein (FTO) has been reported to regulate bone regeneration osteogenic profoundly. However, effect FTO on hSCAPs remains unknown. This study aimed identify potential function hSCAPs’ odontoblastic under normal inflammatory conditions investigate its underlying mechanism...
Citrus yellow mosaic virus (CYMV), a member of the family Caulimoviridae, genus Badnavirus, causes citrus disease, disease that occurs commonly in India. The CYMV genome has been cloned and its complete nucleotide sequence determined. Its DNA is 7559 bp length contains six putative open reading frames (ORFs), all on plus-strand each capable encoding proteins with molecular mass greater than 10 kDa. ORF 3, largest ORF, encodes polyprotein for functions involved movement, assembly replication....
Small non-coding RNAs are essential regulators of gene expression at the transcriptional and posttranscriptional levels. High-throughput sequencing has revealed thousands predicted small RNAs; however, only a few these have been well characterized. Northern blotting is most convincing method for RNA validation.In this study, we improved blot by using biotin-labeled probes. miRNAs siRNAs derived from both Arabidopsis thaliana Oryza sativa were investigated. The results suggest that sensitive...
Petals in rapeseed (Brassica napus) serve multiple functions, including protection of reproductive organs, nutrient acquisition, and attraction pollinators. However, they also cluster densely at the top, forming a thick layer that absorbs reflects considerable amount photosynthetically active radiation. Breeding genotypes with large, small, or even petal-less varieties, requires knowledge primary genes for allelic selection manipulation. our current understanding petal-size regulation is...
Ralstonia solanacearum race 3 biovar 2 strains are considered select agents by the U.S. government because they not endemic to United States and have potential cause brown rot in our potato production fields. Simple accurate methods needed for quick identification prior more discriminating but time-consuming verification methods. We developed a multiplex PCR assay that identifies R. species complex strains, signals whether strain detected is agent, controls false negatives associated with...
ABSTRACT A Ralstonia -infecting jumbo phage was isolated from soil in Maryland, USA. The complete genome sequence determined to be 225,638 bp and has a G+C content of 51.8%.
Brucellosis, caused by Brucella, is a severe zoonotic disease. Conventional IgG antibody-based ELISA testing faces challenges such as false positives and cross-reactivity. In this study, three specific chicken yolk antibodies (IgY) targeting Brucella were isolated from the eggs of immunized hens, method for detecting utilizing these was developed subsequently compared to traditional antibodies. These IgY generated against fusion protein, LPS, whole-cell antigen, their potency evaluated...
The first Ralstonia-infecting bacteriophage from soil of the United States, designated RsoM1USA, was isolated a tomato field in Florida. Electron microscopy revealed that phage RsoM1USA is member genus P2virus family Myoviridae with an icosahedral head about 66 nm diameter, contractile tail 152 length, and long "neck." Phage infected 12 30 tested R. solanacearum species complex strains collected worldwide each three Ralstonia species: solanacearum, pseudosolanacearum, syzygii. completed its...
In this study, microcosms were established to determine the effect of nitrogen (N) and phosphorus (P) on multidrug resistance biofilm-forming abilities Escherichia coli. The expression biofilm-formation-related genes was detected establish correlations between genotype phenotype. Different concentrations N P added make one control group four treatment groups. glass tube method used capabilities. Real-time PCR detect mRNA abundance six in E. No resistant strains isolated from group;...
Summary The restoration fertility complex ( RFC ) was previously identified in Honglian HL )‐cytoplasmic male sterility CMS rice Oryza sativa ), and glycine‐rich protein 162 GRP 162) is responsible for binding to the ‐associated transcript atp6‐orfH79 . Here, we engineered a recombinant containing mitochondrial transit peptide, termed Mt‐ 162, as an artificial restorer of Rf gene. confirmed bind RNA localize mitochondria. transgenic plants showed restored with partially functional pollen. We...
A filamentous bacteriophage, designated ϕRs551, was isolated and purified from the quarantine select agent phytopathogen Ralstonia solanacearum race 3 biovar 2 strain UW551 (phylotype IIB sequevar 1) grown under normal culture conditions. Electron microscopy suggested that ϕRs551 is a member of family Inoviridae, about 1200 nm long 7 wide. has genome 7929 nucleotides containing 14 open reading frames, first virion contains resolvase (ORF13) putative type-2 phage repressor (ORF14). Unlike...
We previously characterized a filamentous lysogenic bacteriophage, ϕRs551, isolated directly from the race 3 biovar 2 phylotype IIB sequevar 1 strain UW551 of R. solanacearum grown under normal culture conditions. The genome ϕRs551 was identified with 100% identity in deposited genomes 11 strains solanacearum, indicating evolutionary and biological importance, ORF14 annotated as putative type-2 repressor. In this study, we determined effect prophage its on virulence competitive fitness...